Construct: ORF ccsbBroadEn_01735

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003146.1_s300c1, BRDN0000396174
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UBE2D1 (7321)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7321 UBE2D1 ubiquitin conjugating enzym... NM_003338.5 100% 100%
2 human 51619 UBE2D4 ubiquitin conjugating enzym... NM_015983.4 77.5% 91.8% (many diffs)
3 human 7321 UBE2D1 ubiquitin conjugating enzym... NM_001204880.1 73.9% 72.7% 0_1ins111;2_3insGGG;4A>C
4 human 7323 UBE2D3 ubiquitin conjugating enzym... NM_001300795.1 64.7% 70.7% (many diffs)
5 human 7323 UBE2D3 ubiquitin conjugating enzym... XM_017008584.2 64.7% 70.7% (many diffs)
6 human 51619 UBE2D4 ubiquitin conjugating enzym... XM_024446796.1 57.3% 68% (many diffs)
7 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... NM_145420.2 89.3% 100% (many diffs)
8 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... XM_006513478.2 81.4% 89.3% (many diffs)
9 mouse 216080 Ube2d1 ubiquitin-conjugating enzym... XM_006513479.2 66.6% 73.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
507
ORF length:
441
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GCTGAAGAGG ATTCAGAAAG AATTGAGTGA TCTACAGCGC GATCCACCTG
121CTCACTGTTC AGCTGGACCT GTGGGAGATG ACTTGTTCCA CTGGCAAGCC ACTATTATGG
181GGCCTCCTGA TAGCGCATAT CAAGGTGGAG TCTTCTTTCT CACTGTACAT TTTCCGACAG
241ATTATCCTTT TAAACCACCA AAGATTGCTT TCACAACAAA AATTTACCAT CCAAACATAA
301ACAGTAATGG AAGTATTTGT CTCGATATTC TGAGGTCACA ATGGTCACCA GCTCTGACTG
361TATCAAAAGT TTTATTGTCC ATATGTTCTC TACTTTGTGA TCCTAATCCA GATGACCCCT
421TAGTACCAGA TATTGCACAA ATCTATAAAT CAGACAAAGA AAAATACAAC AGACATGCAA
481GAGAATGGAC TCAGAAATAT GCAATGTACC CAACTTTCTT GTACAAAGTt ggcattataa
541gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)