Transcript: Mouse NM_145420.2

Mus musculus ubiquitin-conjugating enzyme E2D 1 (Ube2d1), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ube2d1 (216080)
Length:
1448
CDS:
139..582

Additional Resources:

NCBI RefSeq record:
NM_145420.2
NBCI Gene record:
Ube2d1 (216080)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000040894 CCCGCTTTGACTGTATCGAAA pLKO.1 421 CDS 100% 4.950 6.930 N Ube2d1 n/a
2 TRCN0000305462 CGATCCCTTAGTACCAGATAT pLKO_005 486 CDS 100% 13.200 10.560 N Ube2d1 n/a
3 TRCN0000040896 CCGACAGACTATCCCTTTAAA pLKO.1 307 CDS 100% 15.000 10.500 N Ube2d1 n/a
4 TRCN0000317031 CCGACAGACTATCCCTTTAAA pLKO_005 307 CDS 100% 15.000 10.500 N Ube2d1 n/a
5 TRCN0000337494 ATAAACAGCAACGGGAGTATT pLKO_005 370 CDS 100% 13.200 9.240 N Ube2d1 n/a
6 TRCN0000305521 TCGCTGCTCTGTGATCCTAAT pLKO_005 460 CDS 100% 10.800 7.560 N Ube2d1 n/a
7 TRCN0000040895 CGCAAGAGAATGGACTCAGAA pLKO.1 549 CDS 100% 4.950 3.465 N Ube2d1 n/a
8 TRCN0000317030 CGCAAGAGAATGGACTCAGAA pLKO_005 549 CDS 100% 4.950 3.465 N Ube2d1 n/a
9 TRCN0000040893 GCACACTGTATTTCTCAGTAT pLKO.1 1185 3UTR 100% 4.950 3.465 N Ube2d1 n/a
10 TRCN0000316952 GCACACTGTATTTCTCAGTAT pLKO_005 1185 3UTR 100% 4.950 3.465 N Ube2d1 n/a
11 TRCN0000040897 CCACCCAAATATAAACAGCAA pLKO.1 360 CDS 100% 2.640 1.848 N Ube2d1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01735 pDONR223 100% 89.3% 100% None (many diffs) n/a
2 ccsbBroad304_01735 pLX_304 0% 89.3% 100% V5 (many diffs) n/a
3 TRCN0000475410 TCCCCCCACGCGCCCCCGCCATCG pLX_317 71.5% 89.3% 100% V5 (many diffs) n/a
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