Transcript: Human XM_024446796.1

PREDICTED: Homo sapiens ubiquitin conjugating enzyme E2 D4 (putative) (UBE2D4), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UBE2D4 (51619)
Length:
4126
CDS:
356..688

Additional Resources:

NCBI RefSeq record:
XM_024446796.1
NBCI Gene record:
UBE2D4 (51619)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446796.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004123 ATCACCCTAATATCAACAGCA pLKO.1 465 CDS 100% 2.640 3.696 N UBE2D4 n/a
2 TRCN0000284775 CATCCACTTTCCTACAGATTA pLKO_005 403 CDS 100% 13.200 9.240 N UBE2D4 n/a
3 TRCN0000004121 CTAGAGATTTGGGTCATGTTA pLKO.1 1392 3UTR 100% 5.625 3.938 N UBE2D4 n/a
4 TRCN0000272498 CTAGAGATTTGGGTCATGTTA pLKO_005 1392 3UTR 100% 5.625 3.938 N UBE2D4 n/a
5 TRCN0000284777 AGGACCTGTCGGTGATGACTT pLKO_005 313 5UTR 100% 4.950 3.465 N UBE2D4 n/a
6 TRCN0000004124 CACCCTAATATCAACAGCAAT pLKO.1 467 CDS 100% 4.950 3.465 N UBE2D4 n/a
7 TRCN0000272499 CTAGCAAGAGAGTGGACACAA pLKO_005 653 CDS 100% 4.950 3.465 N UBE2D4 n/a
8 TRCN0000004120 GCCGACAGAGAGAAGTACAAC pLKO.1 629 CDS 100% 4.950 3.465 N UBE2D4 n/a
9 TRCN0000284778 CTTGATATCCTGCGGTCTCAG pLKO_005 500 CDS 100% 4.050 2.835 N UBE2D4 n/a
10 TRCN0000004122 CAAGGCCGACAGAGAGAAGTA pLKO.1 625 CDS 100% 4.950 2.970 N UBE2D4 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2518 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2518 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446796.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03347 pDONR223 100% 74.8% 74.8% None 0_1ins111 n/a
2 ccsbBroad304_03347 pLX_304 0% 74.8% 74.8% V5 0_1ins111 n/a
3 TRCN0000474875 GGAGATCAGCCTGCAATCCTGTTA pLX_317 72.2% 74.8% 74.8% V5 0_1ins111 n/a
4 ccsbBroadEn_01736 pDONR223 100% 58.7% 70.7% None (many diffs) n/a
5 ccsbBroad304_01736 pLX_304 0% 58.7% 70.7% V5 (many diffs) n/a
6 TRCN0000476241 GCGGACTCAGCGCCTGTAGTGTGA pLX_317 40.3% 58.7% 70.7% V5 (many diffs) n/a
7 ccsbBroadEn_07114 pDONR223 100% 58.7% 69.3% None (many diffs) n/a
8 ccsbBroad304_07114 pLX_304 0% 58.7% 69.3% V5 (many diffs) n/a
9 TRCN0000467586 GAATGGAAAAAATCAAATTTAACA pLX_317 77% 58.7% 69.3% V5 (many diffs) n/a
10 ccsbBroadEn_01735 pDONR223 100% 57.3% 68% None (many diffs) n/a
11 ccsbBroad304_01735 pLX_304 0% 57.3% 68% V5 (many diffs) n/a
12 TRCN0000475410 TCCCCCCACGCGCCCCCGCCATCG pLX_317 71.5% 57.3% 68% V5 (many diffs) n/a
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