Construct: ORF ccsbBroadEn_01965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016694.1_s300c1, BRDN0000397341
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS20 (8601)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8601 | RGS20 | regulator of G protein sign... | NM_003702.4 | 100% | 100% | |
| 2 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286673.2 | 80.3% | 78.9% | (many diffs) |
| 3 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286675.2 | 71.6% | 70.9% | (many diffs) |
| 4 | human | 8601 | RGS20 | regulator of G protein sign... | NM_001286674.2 | 61.7% | 57% | (many diffs) |
| 5 | human | 8601 | RGS20 | regulator of G protein sign... | NM_170587.4 | 60.5% | 56.5% | (many diffs) |
| 6 | human | 8601 | RGS20 | regulator of G protein sign... | NR_104579.1 | 33.4% | 1_144del;213_214ins149;719_1567del | |
| 7 | mouse | 58175 | Rgs20 | regulator of G-protein sign... | XM_006495554.2 | 52.3% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCG CACGGCGGAC GGAGGCGAGC CGGCCGGGGC TTCCTCCCCG GCCGGCAGGG 121 TGGACGGTGG GCTCCAGATG GGATCAGAGC GGATGGAGAT GCGGAAGCGG CAGATGCCCG 181 CCGCCCAGGA CACACCAGGC GCCGCCCCAG GCCAGCCCGG AGCGGGGAGT CGCGGGTCCA 241 ACGCATGCTG CTTCTGCTGG TGCTGCTGTT GTAGCTGCTC GTGTCTCACT GTTAGAAACC 301 AGGAAGATCA GAGGCCCACA ATAGCTTCCC ACGAACTCAG AGCAGATCTT CCAACCTGGG 361 AAGAAAGCCC TGCTCCTACT CTGGAAGAAG TCAACGCCTG GGCTCAGTCA TTTGACAAAT 421 TAATGGTCAC TCCAGCAGGA AGGAATGCAT TCCGTGAATT CCTCCGAACA GAATTCAGTG 481 AGGAAAATAT GCTCTTCTGG ATGGCCTGTG AGGAACTGAA AAAGGAAGCT AATAAAAACA 541 TTATTGAAGA GAAAGCAAGG ATAATCTATG AAGACTACAT TTCTATACTT TCTCCTAAGG 601 AGGTGAGCTT AGACTCCCGG GTGAGAGAAG TGATCAACAG AAACATGGTG GAGCCATCCC 661 AACACATATT CGATGATGCT CAACTTCAGA TTTACACCCT GATGCACAGA GACTCATATC 721 CTCGATTCAT GAACTCTGCT GTCTATAAGG ACTTGCTTCA GTCCTTATCG GAGAAATCTA 781 TTGAAGCATA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 841 tttgttgcaa cgaac