Construct: ORF ccsbBroadEn_02077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001772.1_s300c1, BRDN0000396161
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1AY (9086)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_004681.4 | 100% | 100% | |
| 2 | human | 1964 | EIF1AX | eukaryotic translation init... | NM_001412.4 | 92.1% | 99.3% | (many diffs) |
| 3 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_001278612.1 | 88.1% | 88.1% | 202_203ins51 |
| 4 | mouse | 13664 | Eif1a | eukaryotic translation init... | NM_010120.5 | 90.9% | 97.9% | (many diffs) |
| 5 | mouse | 666806 | Gm8300 | predicted gene 8300 | NM_001177565.1 | 89.8% | 93% | (many diffs) |
| 6 | mouse | 100039042 | Gm2016 | predicted gene 2016 | NM_001122662.1 | 89.8% | 91.6% | (many diffs) |
| 7 | mouse | 100039052 | Gm2022 | predicted pseudogene 2022 | NM_001177574.1 | 89.5% | 92.3% | (many diffs) |
| 8 | mouse | 100861908 | Gm21319 | predicted gene, 21319 | NM_001270722.1 | 89.5% | 92.3% | (many diffs) |
| 9 | mouse | 435337 | Gm5662 | predicted gene 5662 | NM_001013824.3 | 89.5% | 91.6% | (many diffs) |
| 10 | mouse | 100861898 | Gm21936 | predicted gene, 21936 | XM_006516436.2 | 89.3% | 90.9% | (many diffs) |
| 11 | mouse | 66235 | Eif1ax | eukaryotic translation init... | NM_025437.5 | 89.1% | 99.3% | (many diffs) |
| 12 | mouse | 100042776 | Gm4027 | predicted gene 4027 | NM_001177564.1 | 88.4% | 89.5% | (many diffs) |
| 13 | mouse | 544881 | BB287469 | expressed sequence BB287469 | NM_001177573.1 | 88.4% | 89.5% | (many diffs) |
| 14 | mouse | 100042776 | Gm4027 | predicted gene 4027 | XM_011243969.1 | 88.4% | 89.5% | (many diffs) |
| 15 | mouse | 100503047 | LOC100503047 | eukaryotic translation init... | XM_003085585.1 | 87.3% | 86.2% | (many diffs) |
| 16 | mouse | 100039100 | Gm2046 | predicted gene 2046 | XM_017315327.1 | 77.3% | 78.4% | (many diffs) |
| 17 | mouse | 13664 | Eif1a | eukaryotic translation init... | XM_006525626.3 | 51.5% | 55.5% | (many diffs) |
| 18 | mouse | 266459 | Gm5039 | predicted gene 5039; eukary... | NR_003647.2 | 23.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC CAAGAATAAA GGTAAAGGAG GTAAAAACAG GCGCAGGGGT AAAAATGAGA 121 ATGAATCTGA AAAAAGAGAG TTGGTGTTTA AAGAGGATGG ACAAGAGTAT GCTCAGGTAA 181 TCAAAATGTT GGGAAATGGA CGATTGGAAG CATTGTGTTT TGATGGTGTA AAGAGGTTAT 241 GCCATATCAG AGGGAAATTG AGAAAAAAGG TTTGGATAAA TACATCAGAC ATTATATTGG 301 TTGGTCTACG GGACTATCAG GATAACAAAG CTGATGTAAT TTTAAAGTAC AATGCAGATG 361 AAGCTAGAAG CCTGAAGGCA TATGGCGAGC TTCCAGAACA TGCTAAAATC AATGAAACAG 421 ACACATTTGG TCCTGGAGAT GATGATGAAA TCCAGTTTGA CGATATTGGA GATGATGATG 481 AAGACATTGA TGATATCTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt 541 gcttatcaat ttgttgcaac gaac