Construct: ORF ccsbBroadEn_02245

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013352.1_s300c1, BRDN0000385218
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IST1 (9798)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9798 IST1 IST1 factor associated with... NM_014761.4 100% 100%
2 human 9798 IST1 IST1 factor associated with... NM_001270975.2 98.3% 79% 853_856delGTAG;1085_1098del
3 human 9798 IST1 IST1 factor associated with... NM_001270976.1 94.9% 76.4% 1_39del;892_895delGTAG;1124_1137del
4 human 9798 IST1 IST1 factor associated with... NM_001270977.2 90.4% 74.3% 760_761delGTinsTC;763_764ins89;992_1005del
5 human 9798 IST1 IST1 factor associated with... NM_001270978.2 57.9% 39.7% 0_1ins444;409_412delGTAG;641_654del
6 human 9798 IST1 IST1 factor associated with... NM_001270979.1 57.9% 39.7% 0_1ins444;409_412delGTAG;641_654del
7 mouse 71955 Ist1 increased sodium tolerance ... NM_028018.2 87.4% 76.7% (many diffs)
8 mouse 71955 Ist1 increased sodium tolerance ... XM_017312970.1 84.7% 76.4% (many diffs)
9 mouse 71955 Ist1 increased sodium tolerance ... XM_017312971.1 70.7% 58.5% (many diffs)
10 mouse 71955 Ist1 increased sodium tolerance ... XR_001778470.1 38.8% (many diffs)
11 mouse 71955 Ist1 increased sodium tolerance ... XR_001778469.1 38.2% (many diffs)
12 mouse 71955 Ist1 increased sodium tolerance ... XR_001778468.1 35.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1146
ORF length:
1080
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT GGGCTCTGGA TTTAAAGCTG AGCGCTTAAG AGTGAATTTG AGATTAGTCA
121TAAATCGCCT TAAACTATTG GAGAAAAAGA AAACGGAACT GGCCCAGAAA GCAAGGAAGG
181AGATTGCTGA CTATCTGGCT GCTGGGAAAG ATGAACGAGC TCGGATCCGT GTGGAGCACA
241TTATCCGGGA AGACTACCTC GTGGAGGCCA TGGAGATCCT GGAGCTGTAC TGTGACCTGC
301TGCTGGCTCG GTTTGGCCTT ATCCAGTCTA TGAAGGAACT AGATTCTGGT CTGGCTGAAT
361CTGTGTCTAC ATTGATCTGG GCTGCTCCTC GACTCCAGTC AGAAGTGGCT GAGTTGAAAA
421TAGTTGCTGA TCAGCTCTGT GCCAAGTATA GCAAGGAATA TGGCAAGCTA TGTAGGACCA
481ACCAGATTGG AACTGTGAAT GACAGGCTAA TGCACAAGCT GAGTGTGGAA GCCCCACCCA
541AAATCCTGGT GGAGAGATAC CTGATTGAAA TTGCAAAGAA TTACAACGTA CCCTATGAAC
601CTGACTCTGT GGTCATGGCA GAAGCTCCTC CTGGGGTAGA GACAGATCTT ATTGATGTTG
661GATTCACAGA TGATGTGAAG AAAGGAGGCC CTGGAAGAGG AGGGAGTGGT GGCTTCACAG
721CACCAGTTGG TGGACCTGAT GGAACGGTGC CAATGCCCAT GCCCATGCCC ATGCCTATGC
781CATCTGCAAA TACGCCTTTC TCATATCCAC TGCCAAAGGG ACCATCAGAT TTCAATGGAC
841TGCCAATGGG GACTTATCAG GCCTTTCCCA ATATTCATCC ACCTCAGATA CCAGCAACTC
901CCCCATCGTA TGAATCTATG ACATTAATGC TGATAAGAAT ATCTCTTCTG CACAGATTGT
961TGGTCCTGGA CCCAAGCCAG AAGCCTCTGC AAAGCTTCCT TCCAGACCTG CAGATAACTA
1021TGACAACTTT GTCCTACCAG AGTTGCCATC TGTGCCAGAC ACACTACCAA CTGCATCTGC
1081TGGTGCCAGC ACCTCAGCAT CTGAAGACAT TGACTTTGAT GATCTTTCCC GGAGGTTTGA
1141AGAGCTGCCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1201gttgcaacga ac

Download FASTA (ORF) (Full)