Transcript: Mouse NM_028018.2

Mus musculus increased sodium tolerance 1 homolog (yeast) (Ist1), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ist1 (71955)
Length:
2352
CDS:
127..1215

Additional Resources:

NCBI RefSeq record:
NM_028018.2
NBCI Gene record:
Ist1 (71955)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028018.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000266805 TTCCAGACCTGTGGATAATTA pLKO_005 1053 CDS 100% 15.000 21.000 N Ist1 n/a
2 TRCN0000266804 CATCCGTGTGGAGCACATAAT pLKO_005 285 CDS 100% 13.200 18.480 N Ist1 n/a
3 TRCN0000216431 GTTTCAACCTTGACTCGTAAA pLKO.1 1585 3UTR 100% 10.800 15.120 N Ist1 n/a
4 TRCN0000216630 GAATCTGTTGATGACATTAAT pLKO.1 967 CDS 100% 15.000 10.500 N Ist1 n/a
5 TRCN0000266801 GAATCTGTTGATGACATTAAT pLKO_005 967 CDS 100% 15.000 10.500 N Ist1 n/a
6 TRCN0000266803 TTCAACCTTGACTCGTAAATA pLKO_005 1587 3UTR 100% 15.000 10.500 N Ist1 n/a
7 TRCN0000191849 GAGAGATACCTGATTGAAATT pLKO.1 613 CDS 100% 13.200 9.240 N Ist1 n/a
8 TRCN0000266802 GGGTGGAGACAGATCTTATTG pLKO_005 695 CDS 100% 13.200 9.240 N Ist1 n/a
9 TRCN0000200918 CGTCTCAAACTGTTGGAGAAA pLKO.1 187 CDS 100% 4.950 3.465 N Ist1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028018.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02245 pDONR223 100% 87.4% 76.7% None (many diffs) n/a
2 ccsbBroad304_02245 pLX_304 0% 87.4% 76.7% V5 (many diffs) n/a
3 TRCN0000471238 CTGAAGCGTGGCCCAGTTTTCTCT pLX_317 38.1% 87.4% 76.7% V5 (many diffs) n/a
4 ccsbBroadEn_11417 pDONR223 100% 81.3% 86.6% None (many diffs) n/a
5 ccsbBroad304_11417 pLX_304 0% 81.3% 86.6% V5 (many diffs) n/a
6 TRCN0000472979 CCGGTCCTGCGAGCCAACCTATTG pLX_317 32.2% 81.3% 86.6% V5 (many diffs) n/a
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