Transcript: Human NM_001270975.2

Homo sapiens IST1 factor associated with ESCRT-III (IST1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
IST1 (9798)
Length:
4561
CDS:
75..1175

Additional Resources:

NCBI RefSeq record:
NM_001270975.2
NBCI Gene record:
IST1 (9798)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001270975.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127653 CAACCAGATTGGAACTGTGAA pLKO.1 488 CDS 100% 4.950 6.930 N IST1 n/a
2 TRCN0000323198 ATCAGGCCTTTCCCAATATTC pLKO_005 865 CDS 100% 13.200 10.560 N IST1 n/a
3 TRCN0000191849 GAGAGATACCTGATTGAAATT pLKO.1 561 CDS 100% 13.200 10.560 N Ist1 n/a
4 TRCN0000129488 GATGATCTTTCCCGGAGGTTT pLKO.1 1131 CDS 100% 4.950 3.960 N IST1 n/a
5 TRCN0000323123 GCCAAAGGGACCATCAGATTT pLKO_005 821 CDS 100% 13.200 9.240 N IST1 n/a
6 TRCN0000323122 GCTGAATCTGTGTCTACATTG pLKO_005 363 CDS 100% 10.800 7.560 N IST1 n/a
7 TRCN0000130150 CTGAGCAATTTCTCCTTGTAA pLKO.1 1217 3UTR 100% 5.625 3.938 N IST1 n/a
8 TRCN0000129426 GCTGCTTTCCAGTTCTCTGTT pLKO.1 1328 3UTR 100% 4.950 3.465 N IST1 n/a
9 TRCN0000323121 GCTGCTTTCCAGTTCTCTGTT pLKO_005 1328 3UTR 100% 4.950 3.465 N IST1 n/a
10 TRCN0000130999 CAGACACACTACCAACTGCAT pLKO.1 1069 CDS 100% 2.640 1.848 N IST1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001270975.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02245 pDONR223 100% 98.3% 79% None 853_856delGTAG;1085_1098del n/a
2 ccsbBroad304_02245 pLX_304 0% 98.3% 79% V5 853_856delGTAG;1085_1098del n/a
3 TRCN0000471238 CTGAAGCGTGGCCCAGTTTTCTCT pLX_317 38.1% 98.3% 79% V5 853_856delGTAG;1085_1098del n/a
4 ccsbBroadEn_11417 pDONR223 100% 91.5% 91.5% None 760_852del n/a
5 ccsbBroad304_11417 pLX_304 0% 91.5% 91.5% V5 760_852del n/a
6 TRCN0000472979 CCGGTCCTGCGAGCCAACCTATTG pLX_317 32.2% 91.5% 91.5% V5 760_852del n/a
Download CSV