Construct: ORF ccsbBroadEn_02332

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003954.1_s300c1, BRDN0000387862
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MBNL2 (10150)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10150 MBNL2 muscleblind like splicing r... NM_144778.4 100% 100%
2 human 10150 MBNL2 muscleblind like splicing r... XM_005254022.3 96.7% 96.7% 957_958ins36
3 human 10150 MBNL2 muscleblind like splicing r... XM_005254018.3 95.3% 95.3% 803_856del
4 human 10150 MBNL2 muscleblind like splicing r... NM_001306070.1 92.4% 88.9% (many diffs)
5 human 10150 MBNL2 muscleblind like splicing r... XM_005254021.3 92.2% 92.2% 803_856del;1011_1012ins36
6 human 10150 MBNL2 muscleblind like splicing r... NM_207304.2 91.9% 86.6% (many diffs)
7 human 10150 MBNL2 muscleblind like splicing r... XM_006719912.3 88.3% 84.9% (many diffs)
8 human 10150 MBNL2 muscleblind like splicing r... XM_005254019.3 87.7% 82.6% (many diffs)
9 human 10150 MBNL2 muscleblind like splicing r... XM_024449301.1 78.4% 70.7% (many diffs)
10 human 10150 MBNL2 muscleblind like splicing r... XM_024449300.1 75.7% 69.9% (many diffs)
11 mouse 105559 Mbnl2 muscleblind like splicing f... XM_006518375.3 93.2% 97.8% (many diffs)
12 mouse 105559 Mbnl2 muscleblind like splicing f... NM_207515.2 90% 94.5% (many diffs)
13 mouse 105559 Mbnl2 muscleblind like splicing f... XM_006518371.3 88.9% 93.2% (many diffs)
14 mouse 105559 Mbnl2 muscleblind like splicing f... NM_175341.4 85.8% 90.1% (many diffs)
15 mouse 105559 Mbnl2 muscleblind like splicing f... XM_006518374.3 85.8% 90.1% (many diffs)
16 mouse 105559 Mbnl2 muscleblind like splicing f... XM_006518376.3 84.3% 84.5% (many diffs)
17 mouse 105559 Mbnl2 muscleblind like splicing f... XM_006518373.1 80.4% 80.6% (many diffs)
18 mouse 105559 Mbnl2 muscleblind like splicing f... XR_001781083.1 16.8% (many diffs)
19 mouse 105559 Mbnl2 muscleblind like splicing f... XR_001781084.1 16.5% (many diffs)
20 mouse 105559 Mbnl2 muscleblind like splicing f... XR_001781082.1 16.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1170
ORF length:
1101
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCTTTGAAC GTTGCCCCAG TCAGAGATAC AAAATGGCTG ACATTAGAAG
121TCTGCAGACA GTTTCAAAGA GGAACATGCT CACGCTCTGA TGAAGAATGC AAATTTGCTC
181ATCCCCCCAA AAGTTGTCAG GTTGAAAATG GAAGAGTAAT TGCCTGCTTT GATTCCCTAA
241AGGGCCGTTG TTCGAGAGAG AACTGCAAGT ATCTTCACCC TCCGACACAC TTAAAAACTC
301AACTAGAAAT TAATGGAAGG AACAATTTGA TTCAGCAAAA AACTGCAGCA GCAATGCTTG
361CCCAGCAGAT GCAATTTATG TTTCCAGGAA CACCACTTCA TCCAGTGCCC ACTTTCCCTG
421TAGGTCCCGC GATAGGGACA AATACGGCTA TTAGCTTTGC TCCTTACCTA GCACCTGTAA
481CCCCTGGAGT TGGGTTGGTC CCAACGGAAA TTCTGCCCAC CACGCCTGTT ATTGTTCCCG
541GAAGTCCACC GGTCACTGTC CCGGGCTCAA CTGCAACTCA GAAACTTCTC AGGACTGACA
601AACTGGAGGT ATGCAGGGAG TTCCAGCGAG GAAACTGTGC CCGGGGAGAG ACCGACTGCC
661GCTTTGCACA CCCCGCAGAC AGCACCATGA TCGACACAAG TGACAACACC GTAACCGTTT
721GTATGGATTA CATAAAGGGG CGTTGCATGA GGGAGAAATG CAAATATTTT CACCCTCCTG
781CACACTTGCA GGCCAAAATC AAAGCTGCGC AGCACCAAGC CAACCAAGCT GCGGTGGCCG
841CCCAGGCAGC CGCGGCCGCG GCCACAGTCA TGGCCTTTCC CCCTGGTGCT CTTCATCCTT
901TACCAAAGAG ACAAGCACTT GAAAAAAGCA ATGGTACCAG CGCGGTCTTT AACCCCAGCG
961TCTTGCACTA CCAGCAGGCT CTCACCAGCG CACAGTTGCA GCAACACGCC GCGTTCATTC
1021CAACAGGGTC AGTTTTGTGC ATGACACCCG CTACCAGTAT TGTACCCATG ATGCACAGCG
1081CTACGTCCGC CACTGTCTCT GCAGCAACAA CTCCTGCAAC AAGTGTCCCC TTCGCAGCAA
1141CAGCCACAGC CAATCAGATA ATTCTGAAAT TGCCAACTTT CTTGTACAAA GTtggcatta
1201taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)