Construct: ORF ccsbBroadEn_02759

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016769.1_s300c1, BRDN0000457474
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DICER1 (23405)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23405 DICER1 dicer 1, ribonuclease III NM_001271282.3 100% 100%
2 human 23405 DICER1 dicer 1, ribonuclease III NM_001291628.1 100% 100%
3 human 23405 DICER1 dicer 1, ribonuclease III NM_030621.4 100% 100%
4 human 23405 DICER1 dicer 1, ribonuclease III NM_177438.2 100% 100%
5 human 23405 DICER1 dicer 1, ribonuclease III XM_011536599.2 100% 100%
6 human 23405 DICER1 dicer 1, ribonuclease III XM_011536600.3 100% 100%
7 human 23405 DICER1 dicer 1, ribonuclease III XM_011536601.3 100% 100%
8 human 23405 DICER1 dicer 1, ribonuclease III XM_011536602.3 100% 100%
9 human 23405 DICER1 dicer 1, ribonuclease III XM_017021120.2 100% 100%
10 human 23405 DICER1 dicer 1, ribonuclease III XM_017021121.2 100% 100%
11 human 23405 DICER1 dicer 1, ribonuclease III NM_001195573.1 95.1% 93.2% 5362_5363ins163;5487_5488ins116
12 human 23405 DICER1 dicer 1, ribonuclease III XM_011536604.2 92.9% 92.5% 0_1ins274;32_33ins131
13 human 23405 DICER1 dicer 1, ribonuclease III XM_017021122.2 92.9% 92.5% 0_1ins274;32_33ins131
14 human 23405 DICER1 dicer 1, ribonuclease III XM_017021123.2 92.9% 92.5% 0_1ins274;32_33ins131
15 human 23405 DICER1 dicer 1, ribonuclease III XM_011536605.2 74.3% 74.3% 0_1ins1479
16 mouse 192119 Dicer1 dicer 1, ribonuclease type III NM_148948.2 88.4% 92.4% (many diffs)
17 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_006515608.3 88.4% 92.4% (many diffs)
18 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_006515609.3 88.4% 92.4% (many diffs)
19 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_006515611.3 88.4% 92.4% (many diffs)
20 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_017314997.1 82.7% 85.1% (many diffs)
21 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_017314998.1 81.8% 85.6% (many diffs)
22 mouse 192119 Dicer1 dicer 1, ribonuclease type III XM_017314999.1 65.6% 68.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
5835
ORF length:
5766
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GAAAAGCCCT GCTTTGCAAC CCCTCAGCAT GGCAGGCCTG CAGCTCATGA
121CCCCTGCTTC CTCACCAATG GGTCCTTTCT TTGGACTGCC ATGGCAACAA GAAGCAATTC
181ATGATAACAT TTATACGCCA AGAAAATATC AGGTTGAACT GCTTGAAGCA GCTCTGGATC
241ATAATACCAT CGTCTGTTTA AACACTGGCT CAGGGAAGAC ATTTATTGCA GTACTACTCA
301CTAAAGAGCT GTCCTATCAG ATCAGGGGAG ACTTCAGCAG AAATGGAAAA AGGACGGTGT
361TCTTGGTCAA CTCTGCAAAC CAGGTTGCTC AACAAGTGTC AGCTGTCAGA ACTCATTCAG
421ATCTCAAGGT TGGGGAATAC TCAAACCTAG AAGTAAATGC ATCTTGGACA AAAGAGAGAT
481GGAACCAAGA GTTTACTAAG CACCAGGTTC TCATTATGAC TTGCTATGTC GCCTTGAATG
541TTTTGAAAAA TGGTTACTTA TCACTGTCAG ACATTAACCT TTTGGTGTTT GATGAGTGTC
601ATCTTGCAAT CCTAGACCAC CCCTATCGAG AAATTATGAA GCTCTGTGAA AATTGTCCAT
661CATGTCCTCG CATTTTGGGA CTAACTGCTT CCATTTTAAA TGGGAAATGT GATCCAGAGG
721AATTGGAAGA AAAGATTCAG AAACTAGAGA AAATTCTTAA GAGTAATGCT GAAACTGCAA
781CTGACCTGGT GGTCTTAGAC AGGTATACTT CTCAGCCATG TGAGATTGTG GTGGATTGTG
841GACCATTTAC TGACAGAAGT GGGCTTTATG AAAGACTGCT GATGGAATTA GAAGAAGCAC
901TTAATTTTAT CAATGATTGT AATATATCTG TACATTCAAA AGAAAGAGAT TCTACTTTAA
961TTTCGAAACA GATACTATCA GACTGTCGTG CCGTATTGGT AGTTCTGGGA CCCTGGTGTG
1021CAGATAAAGT AGCTGGAATG ATGGTAAGAG AACTACAGAA ATACATCAAA CATGAGCAAG
1081AGGAGCTGCA CAGGAAATTT TTATTGTTTA CAGACACTTT CCTAAGGAAA ATACATGCAC
1141TATGTGAAGA GCACTTCTCA CCTGCCTCAC TTGACCTGAA ATTTGTAACT CCTAAAGTAA
1201TCAAACTGCT CGAAATCTTA CGCAAATATA AACCATATGA GCGACAGCAG TTTGAAAGCG
1261TTGAGTGGTA TAATAATAGA AATCAGGATA ATTATGTGTC ATGGAGTGAT TCTGAGGATG
1321ATGATGAGGA TGAAGAAATT GAAGAAAAAG AGAAGCCAGA GACAAATTTT CCTTCTCCTT
1381TTACCAACAT TTTGTGCGGA ATTATTTTTG TGGAAAGAAG ATACACAGCA GTTGTCTTAA
1441ACAGATTGAT AAAGGAAGCT GGCAAACAAG ATCCAGAGCT GGCTTATATC AGTAGCAATT
1501TCATAACTGG ACATGGCATT GGGAAGAATC AGCCTCGCAA CAAACAGATG GAAGCAGAAT
1561TCAGAAAACA GGAAGAGGTA CTTAGGAAAT TTCGAGCACA TGAGACCAAC CTGCTTATTG
1621CAACAAGTAT TGTAGAAGAG GGTGTTGATA TACCAAAATG CAACTTGGTG GTTCGTTTTG
1681ATTTGCCCAC AGAATATCGA TCCTATGTTC AATCTAAAGG AAGAGCAAGG GCACCCATCT
1741CTAATTATAT AATGTTAGCG GATACAGACA AAATAAAAAG TTTTGAAGAA GACCTTAAAA
1801CCTACAAAGC TATTGAAAAG ATCTTGAGAA ACAAGTGTTC CAAGTCGGTT GATACTGGTG
1861AGACTGACAT TGATCCTGTC ATGGATGATG ATGACGTTTT CCCACCATAT GTGTTGAGGC
1921CTGACGATGG TGGTCCACGA GTCACAATCA ACACGGCCAT TGGACACATC AATAGATACT
1981GTGCTAGATT ACCAAGTGAT CCGTTTACTC ATCTAGCTCC TAAATGCAGA ACCCGAGAGT
2041TGCCTGATGG TACATTTTAT TCAACTCTTT ATCTGCCAAT TAACTCACCT CTTCGAGCCT
2101CCATTGTTGG TCCACCAATG AGCTGTGTAC GATTGGCTGA AAGAGTTGTA GCTCTCATTT
2161GCTGTGAGAA ACTGCACAAA ATTGGCGAAC TGGATGACCA TTTGATGCCA GTTGGGAAAG
2221AGACTGTTAA ATATGAAGAG GAGCTTGATT TGCATGATGA AGAAGAGACC AGTGTTCCAG
2281GAAGACCAGG TTCCACGAAA CGAAGGCAGT GCTACCCAAA AGCAATTCCA GAGTGTTTGA
2341GGGATAGTTA TCCCAGACCT GATCAGCCCT GTTACCTGTA TGTGATAGGA ATGGTTTTAA
2401CTACACCTTT ACCTGATGAA CTCAACTTTA GAAGGCGGAA GCTCTATCCT CCTGAAGATA
2461CCACAAGATG CTTTGGAATA CTGACGGCCA AACCCATACC TCAGATTCCA CACTTTCCTG
2521TGTACACACG CTCTGGAGAG GTTACCATAT CCATTGAGTT GAAGAAGTCT GGTTTCATGT
2581TGTCTCTACA AATGCTTGAG TTGATTACAA GACTTCACCA GTATATATTC TCACATATTC
2641TTCGGCTTGA AAAACCTGCA CTAGAATTTA AACCTACAGA CGCTGATTCA GCATACTGTG
2701TTCTACCTCT TAATGTTGTT AATGACTCCA GCACTTTGGA TATTGACTTT AAATTCATGG
2761AAGATATTGA GAAGTCTGAA GCTCGCATAG GCATTCCCAG TACAAAGTAT ACAAAAGAAA
2821CACCCTTTGT TTTTAAATTA GAAGATTACC AAGATGCCGT TATCATTCCA AGATATCGCA
2881ATTTTGATCA GCCTCATCGA TTTTATGTAG CTGATGTGTA CACTGATCTT ACCCCACTCA
2941GTAAATTTCC TTCCCCTGAG TATGAAACTT TTGCAGAATA TTATAAAACA AAGTACAACC
3001TTGACCTAAC CAATCTCAAC CAGCCACTGC TGGATGTGGA CCACACATCT TCAAGACTTA
3061ATCTTTTGAC ACCTCGACAT TTGAATCAGA AGGGGAAAGC GCTTCCTTTA AGCAGTGCTG
3121AGAAGAGGAA AGCCAAATGG GAAAGTCTGC AGAATAAACA GATACTGGTT CCAGAACTCT
3181GTGCTATACA TCCAATTCCA GCATCACTGT GGAGAAAAGC TGTTTGTCTC CCCAGCATAC
3241TTTATCGCCT TCACTGCCTT TTGACTGCAG AGGAGCTAAG AGCCCAGACT GCCAGCGATG
3301CTGGCGTGGG AGTCAGATCA CTTCCTGCGG ATTTTAGATA CCCTAACTTA GACTTCGGGT
3361GGAAAAAATC TATTGACAGC AAATCTTTCA TCTCAATTTC TAACTCCTCT TCAGCTGAAA
3421ATGATAATTA CTGTAAGCAC AGCACAATTG TCCCTGAAAA TGCTGCACAT CAAGGTGCTA
3481ATAGAACCTC CTCTCTAGAA AATCATGACC AAATGTCTGT GAACTGCAGA ACGTTGCTCA
3541GCGAGTCCCC TGGTAAGCTC CACGTTGAAG TTTCAGCAGA TCTTACAGCA ATTAATGGTC
3601TTTCTTACAA TCAAAATCTC GCCAATGGCA GTTATGATTT AGCTAACAGA GACTTTTGCC
3661AAGGAAATCA GCTAAATTAC TACAAGCAGG AAATACCCGT GCAACCAACT ACCTCATATT
3721CCATTCAGAA TTTATACAGT TACGAGAACC AGCCCCAGCC CAGCGATGAA TGTACTCTCC
3781TGAGTAATAA ATACCTTGAT GGAAATGCTA ACAAATCTAC CTCAGATGGA AGTCCTGTGA
3841TGGCCGTAAT GCCTGGTACG ACAGACACTA TTCAAGTGCT CAAGGGCAGG ATGGATTCTG
3901AGCAGAGCCC TTCTATTGGG TACTCCTCAA GGACTCTTGG CCCCAATCCT GGACTTATTC
3961TTCAGGCTTT GACTCTGTCA AACGCTAGTG ATGGATTTAA CCTGGAGCGG CTTGAAATGC
4021TTGGCGACTC CTTTTTAAAG CATGCCATCA CCACATATCT ATTTTGCACT TACCCTGATG
4081CGCATGAGGG CCGCCTTTCA TATATGAGAA GCAAAAAGGT CAGCAACTGT AATCTGTATC
4141GCCTTGGAAA AAAGAAGGGA CTACCCAGCC GCATGGTGGT GTCAATATTT GATCCCCCTG
4201TGAATTGGCT TCCTCCTGGT TATGTAGTAA ATCAAGACAA AAGCAACACA GATAAATGGG
4261AAAAAGATGA AATGACAAAA GACTGCATGC TGGCGAATGG CAAACTGGAT GAGGATTACG
4321AGGAGGAGGA TGAGGAGGAG GAGAGCCTGA TGTGGAGGGC TCCGAAGGAA GAGGCTGACT
4381ATGAAGATGA TTTCCTGGAG TATGATCAGG AACATATCAG ATTTATAGAT AATATGTTAA
4441TGGGGTCAGG AGCTTTTGTA AAGAAAATCT CTCTTTCTCC TTTTTCAACC ACTGATTCTG
4501CATATGAATG GAAAATGCCC AAAAAATCCT CCTTAGGTAG TATGCCATTT TCATCAGATT
4561TTGAGGATTT TGACTACAGC TCTTGGGATG CAATGTGCTA TCTGGATCCT AGCAAAGCTG
4621TTGAAGAAGA TGACTTTGTG GTGGGGTTCT GGAATCCATC AGAAGAAAAC TGTGGTGTTG
4681ACACGGGAAA GCAGTCCATT TCTTACGACT TGCACACTGA GCAGTGTATT GCTGACAAAA
4741GCATAGCGGA CTGTGTGGAA GCCCTGCTGG GCTGCTATTT AACCAGCTGT GGGGAGAGGG
4801CTGCTCAGCT TTTCCTCTGT TCACTGGGGC TGAAGGTGCT CCCGGTAATT AAAAGGACTG
4861ATCGGGAAAA GGCCCTGTGC CCTACTCGGG AGAATTTCAA CAGCCAACAA AAGAACCTTT
4921CAGTGAGCTG TGCTGCTGCT TCTGTGGCCA GTTCACGCTC TTCTGTATTG AAAGACTCGG
4981AATATGGTTG TTTGAAGATT CCACCAAGAT GTATGTTTGA TCATCCAGAT GCAGATAAAA
5041CACTGAATCA CCTTATATCG GGGTTTGAAA ATTTTGAAAA GAAAATCAAC TACAGATTCA
5101AGAATAAGGC TTACCTTCTC CAGGCTTTTA CACATGCCTC CTACCACTAC AATACTATCA
5161CTGATTGTTA CCAGCGCTTA GAATTCCTGG GAGATGCGAT TTTGGACTAC CTCATAACCA
5221AGCACCTTTA TGAAGACCCG CGGCAGCACT CCCCGGGGGT CCTGACAGAC CTGCGGTCTG
5281CCCTGGTCAA CAACACCATC TTTGCATCGC TGGCTGTAAA GTACGACTAC CACAAGTACT
5341TCAAAGCTGT CTCTCCTGAG CTCTTCCATG TCATTGATGA CTTTGTGCAG TTTCAGCTTG
5401AGAAGAATGA AATGCAAGGA ATGGATTCTG AGCTTAGGAG ATCTGAGGAG GATGAAGAGA
5461AAGAAGAGGA TATTGAAGTT CCAAAGGCCA TGGGGGATAT TTTTGAGTCG CTTGCTGGTG
5521CCATTTACAT GGATAGTGGG ATGTCACTGG AGACAGTCTG GCAGGTGTAC TATCCCATGA
5581TGCGGCCACT AATAGAAAAG TTTTCTGCAA ATGTACCCCG TTCCCCTGTG CGAGAATTGC
5641TTGAAATGGA ACCAGAAACT GCCAAATTTA GCCCGGCTGA GAGAACTTAC GACGGGAAGG
5701TCAGAGTCAC TGTGGAAGTA GTAGGAAAGG GGAAATTTAA AGGTGTTGGT CGAAGTTACA
5761GGATTGCCAA ATCTGCAGCA GCAAGAAGAG CCCTCCGAAG CCTCAAAGCT AATCAACCTC
5821AGGTTCCCAA TAGCTTGCCA ACTTTCTTGT ACAAAGTTGG cattataaga aagcattgct
5881tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)