Construct: ORF ccsbBroadEn_03476

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018235.1_s300c1, BRDN0000396251
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC66A1 (54896)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54896 SLC66A1 solute carrier family 66 me... NM_001040126.2 100% 100%
2 human 54896 SLC66A1 solute carrier family 66 me... NM_001287531.2 79.6% 79.6% 0_1ins138
3 human 54896 SLC66A1 solute carrier family 66 me... XM_006710710.3 79.6% 79.6% 0_1ins138
4 human 54896 SLC66A1 solute carrier family 66 me... XM_006710711.2 79.6% 79.6% 0_1ins138
5 human 54896 SLC66A1 solute carrier family 66 me... NM_001040125.2 77.6% 77.6% 1_195del
6 human 54896 SLC66A1 solute carrier family 66 me... NM_017765.3 77.6% 77.6% 1_195del
7 human 54896 SLC66A1 solute carrier family 66 me... XM_005245916.2 77.6% 77.6% 1_195del
8 human 54896 SLC66A1 solute carrier family 66 me... XM_011541634.3 51.9% 49.3% (many diffs)
9 human 54896 SLC66A1 solute carrier family 66 me... XR_001737253.1 37.8% 1_815del;1494_1792del
10 human 54896 SLC66A1 solute carrier family 66 me... NR_109848.2 25.6% 1_467del;1146_2639del
11 human 54896 SLC66A1 solute carrier family 66 me... XR_001737254.1 10.6% (many diffs)
12 mouse 212555 Pqlc2 PQ loop repeat containing 2 NM_145384.2 66.2% 67.2% (many diffs)
13 mouse 212555 Pqlc2 PQ loop repeat containing 2 XM_011250220.2 66.2% 67.2% (many diffs)
14 mouse 212555 Pqlc2 PQ loop repeat containing 2 XM_011250221.2 66.2% 67.2% (many diffs)
15 mouse 212555 Pqlc2 PQ loop repeat containing 2 XM_011250222.2 66.2% 67.2% (many diffs)
16 mouse 212555 Pqlc2 PQ loop repeat containing 2 XM_017320095.1 66.2% 67.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
744
ORF length:
678
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CCAGGCGCTG TCCCTGTGGT TCCTCCTGGG CTGGATTGGC GGAGACTCCT
121GCAACCTCAT CGGCTCCTTC CTTGCTGACC AGCTGCCCCT GCAGACCTAC ACGGCTGTGT
181ATTATGTCTT GGCAGACCTG GTGATGCTGA CGCTGTACTT TTACTACAAG TTCAGGACGC
241GCCCCTCTCT GTTGTCTGCC CCCATCAACT CCGTGCTGTT GTTCCTCATG GGGATGGCGT
301GCGCCACACC GCTGCTGAGT GCTGCTGGGC CCGTGGCTGC CCCTAGGGAA GCCTTCCGGG
361GGCGGGCGCT CCTGTCCGTG GAGTCGGGCA GCAAGCCCTT CACCCGGCAG GAAGTCATTG
421GCTTCGTCAT CGGCTCCATC TCCAGCGTGT TGTACCTGCT TTCCCGGCTG CCTCAGATCC
481GCACCAACTT CCTCCGGAAG TCCACCCAGG GGATCTCCTA CTCTCTGTTC GCGCTGGTGA
541TGCTGGGGAA CACGCTGTAT GGGCTGAGCG TGCTGCTCAA AAACCCCGAG GAGGGCCAGA
601GCGAGGGCAG CTACCTGCTG CACCACCTGC CCTGGCTTGT GGGCAGCCTG GGCGTGCTGC
661TGCTCGACAC CATCATCTCC ATCCAGTTCC TGGTGTACAG GCGCAGCACC GCCGCCTCGG
721AGCTTGAGCC CCTCCTCCCC AGCTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa
781agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)