Transcript: Mouse NM_145384.2

Mus musculus PQ loop repeat containing 2 (Pqlc2), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Pqlc2 (212555)
Length:
2047
CDS:
279..1160

Additional Resources:

NCBI RefSeq record:
NM_145384.2
NBCI Gene record:
Pqlc2 (212555)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145384.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196084 GCCTCAGATTCGCACCAATTT pLKO.1 878 CDS 100% 13.200 18.480 N Pqlc2 n/a
2 TRCN0000184598 GCTGACGCTGTACTTCCATTA pLKO.1 614 CDS 100% 10.800 15.120 N Pqlc2 n/a
3 TRCN0000340973 GCTGACGCTGTACTTCCATTA pLKO_005 614 CDS 100% 10.800 15.120 N Pqlc2 n/a
4 TRCN0000179752 CCAATTTCATACGGCAGTCAA pLKO.1 892 CDS 100% 4.950 6.930 N Pqlc2 n/a
5 TRCN0000352468 CCTCAGATTCGCACCAATTTC pLKO_005 879 CDS 100% 13.200 10.560 N Pqlc2 n/a
6 TRCN0000340898 GACTGGATACAACCTAGTAAA pLKO_005 1490 3UTR 100% 13.200 9.240 N Pqlc2 n/a
7 TRCN0000340897 GTCTGTGGAGCCAGGCAATAA pLKO_005 782 CDS 100% 13.200 9.240 N Pqlc2 n/a
8 TRCN0000340974 AGAAGGAGGTCATCGGCTTTG pLKO_005 814 CDS 100% 6.000 4.200 N Pqlc2 n/a
9 TRCN0000122797 GCAGACCTACACGGCTGTGTA pLKO.1 569 CDS 100% 0.165 0.099 N SLC66A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145384.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03476 pDONR223 100% 66.2% 67.2% None (many diffs) n/a
2 ccsbBroad304_03476 pLX_304 0% 66.2% 67.2% V5 (many diffs) n/a
3 TRCN0000466893 TCCCGTTGTTACCCCTGCTCGTAG pLX_317 47.9% 66.2% 67.2% V5 (many diffs) n/a
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