Transcript: Human XM_005245916.2

PREDICTED: Homo sapiens solute carrier family 66 member 1 (SLC66A1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SLC66A1 (54896)
Length:
4979
CDS:
136..1011

Additional Resources:

NCBI RefSeq record:
XM_005245916.2
NBCI Gene record:
SLC66A1 (54896)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005245916.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143603 GAGAACAGTCTCAGCACAATT pLKO.1 3714 3UTR 100% 13.200 9.240 N SLC66A1 n/a
2 TRCN0000121655 GCCGAGATTTCAGGATAAGTT pLKO.1 3960 3UTR 100% 5.625 3.938 N SLC66A1 n/a
3 TRCN0000122689 GTGATGCTGACGCTGTACTTT pLKO.1 466 CDS 100% 5.625 3.938 N SLC66A1 n/a
4 TRCN0000444798 CTCCATCTCCAGCGTGTTGTA pLKO_005 699 CDS 100% 4.950 3.465 N SLC66A1 n/a
5 TRCN0000424568 ATATGGGATGTGTTGGGTGAA pLKO_005 199 CDS 100% 4.050 2.835 N SLC66A1 n/a
6 TRCN0000142629 CCGTTTCTTCATCCCATGAGA pLKO.1 4656 3UTR 100% 3.000 2.100 N SLC66A1 n/a
7 TRCN0000141716 GAAGCCGTTTCTTCATCCCAT pLKO.1 4652 3UTR 100% 2.640 1.848 N SLC66A1 n/a
8 TRCN0000143213 GTATTATGTCTTGGCAGACCT pLKO.1 444 CDS 100% 2.640 1.848 N SLC66A1 n/a
9 TRCN0000122797 GCAGACCTACACGGCTGTGTA pLKO.1 426 CDS 100% 0.165 0.116 N SLC66A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005245916.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03476 pDONR223 100% 77.6% 77.6% None 1_195del n/a
2 ccsbBroad304_03476 pLX_304 0% 77.6% 77.6% V5 1_195del n/a
3 TRCN0000466893 TCCCGTTGTTACCCCTGCTCGTAG pLX_317 47.9% 77.6% 77.6% V5 1_195del n/a
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