Construct: ORF ccsbBroadEn_03626

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003678.1_s300c1, BRDN0000389886
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MFN1 (55669)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55669 MFN1 mitofusin 1 NM_033540.3 100% 100%
2 human 55669 MFN1 mitofusin 1 XM_005247596.4 100% 100%
3 human 55669 MFN1 mitofusin 1 XM_011512963.3 80.1% 80.1% 0_1ins441
4 mouse 67414 Mfn1 mitofusin 1 NM_024200.4 86.6% 90.5% (many diffs)
5 mouse 67414 Mfn1 mitofusin 1 XR_001783736.1 63.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2289
ORF length:
2223
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC AGAACCTGTT TCTCCACTGA AGCACTTTGT GCTGGCTAAG AAGGCGATTA
121CTGCAATCTT TGACCAGTTA CTGGAGTTTG TTACTGAAGG ATCACATTTT GTTGAAGCAA
181CATATAAGAA TCCGGAACTT GATCGAATAG CCACTGAAGA TGATCTGGTA GAAATGCAAG
241GATATAAAGA CAAGCTTTCC ATCATTGGTG AGGTGCTATC TCGGAGACAC ATGAAGGTGG
301CATTTTTTGG CAGGACAAGC AGTGGGAAGA GCTCTGTTAT CAATGCAATG TTGTGGGATA
361AAGTTCTCCC TAGTGGGATT GGCCATATAA CCAATTGCTT CCTAAGTGTT GAAGGAACTG
421ATGGAGATAA AGCCTATCTT ATGACAGAAG GATCAGATGA AAAAAAGAGT GTGAAGACAG
481TTAATCAACT GGCCCATGCC CTTCACATGG ACAAAGATTT GAAAGCTGGC TGTCTTGTAC
541GTGTGTTTTG GCCAAAAGCA AAATGTGCCC TCTTGAGAGA TGACCTGGTG TTAGTAGACA
601GTCCAGGCAC AGATGTCACT ACAGAGCTGG ATAGCTGGAT TGATAAGTTT TGCCTAGATG
661CTGATGTCTT TGTTTTGGTC GCAAACTCTG AATCAACACT AATGAATACG GAAAAACACT
721TTTTTCACAA GGTGAATGAG CGGCTTTCCA AGCCTAATAT TTTCATTCTC AATAATCGTT
781GGGATGCCTC TGCATCAGAG CCAGAATATA TGGAAGACGT ACGCAGACAG CACATGGAAA
841GATGCCTGCA TTTCTTGGTG GAGGAGCTCA AAGTTGTAAA TGCTTTAGAA GCACAGAATC
901GTATCTTCTT TGTTTCAGCA AAGGAAGTTC TTAGTGCTAG AAAGCAAAAA GCACAGGGGA
961TGCCAGAAAG TGGTGTGGCA CTTGCTGAAG GATTTCATGC AAGATTACAG GAATTTCAGA
1021ATTTTGAACA AATCTTTGAG GAGTGTATCT CGCAGTCAGC AGTGAAAACA AAGTTCGAAC
1081AGCACACTAT CAGAGCTAAA CAGATACTAG CTACTGTGAA AAACATAATG GATTCAGTAA
1141ACCTGGCAGC TGAAGATAAA AGGCATTATT CAGTGGAAGA GAGGGAAGAC CAAATTGATA
1201GACTGGACTT TATTCGAAAC CAGATGAACC TTTTAACACT GGATGTTAAG AAAAAAATCA
1261AGGAGGTTAC CGAGGAGGTG GCAAACAAAG TTTCATGTGC AATGACAGAT GAAATTTGTC
1321GACTGTCTGT TTTGGTTGAT GAATTTTGTT CAGAGTTTCA TCCTAATCCA GATGTATTAA
1381AAATATATAA AAGTGAATTA AATAAGCACA TAGAGGATGG TATGGGAAGA AATTTGGCTG
1441ATCGATGCAC CGATGAAGTA AACGCCTTAG TGCTTCAGAC CCAGCAAGAA ATTATTGAAA
1501ATTTGAAGCC ATTACTTCCA GCTGGTATAC AGGATAAACT ACATACACTG ATCCCTTGCA
1561AGAAATTTGA TCTCAGTTAT AATCTAAATT ACCACAAGTT ATGTTCAGAT TTTCAAGAGG
1621ATATTGTATT TCGTTTTTCC CTGGGCTGGT CTTCCCTTGT ACATCGATTT TTGGGCCCTA
1681GAAATGCTCA AAGGGTGCTC CTAGGATTAT CAGAGCCTAT CTTTCAGCTC CCTAGATCTT
1741TAGCTTCTAC TCCCACTGCT CCTACCACTC CAGCAACGCC AGATAATGCA TCACAGGAAG
1801AACTCATGAT TACATTAGTA ACAGGATTGG CGTCCGTTAC ATCTAGAACT TCTATGGGCA
1861TCATTATTGT TGGAGGAGTG ATTTGGAAAA CTATAGGCTG GAAACTCCTA TCTGTTTCAT
1921TAACTATGTA TGGAGCTTTG TATCTTTATG AAAGACTGAG CTGGACCACC CATGCCAAGG
1981AGCGAGCCTT TAAACAGCAG TTTGTAAACT ATGCAACTGA AAAACTGAGG ATGATTGTTA
2041GCTCCACGAG TGCAAACTGC AGTCACCAAG TAAAACAACA AATAGCTACC ACTTTTGCTC
2101GCCTGTGCCA ACAAGTTGAT ATTACTCAAA AACAGCTGGA AGAAGAAATT GCTAGATTAC
2161CCAAAGAAAT AGATCAGTTG GAGAAAATAC AAAACAATTC AAAGCTCTTA AGAAATAAAG
2221CTGTTCAACT TGAAAATGAG CTGGAGAATT TTACTAAGCA GTTTCTACCT TCAAGCAATG
2281AAGAATCCTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
2341tttgttgcaa cgaac

Download FASTA (ORF) (Full)