Construct: ORF ccsbBroadEn_04181

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006289.1_s300c1, BRDN0000456885
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IFT74 (80173)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80173 IFT74 intraflagellar transport 74 NM_001099224.2 100% 100%
2 human 80173 IFT74 intraflagellar transport 74 NM_001349928.2 65.4% 63.2% (many diffs)
3 human 80173 IFT74 intraflagellar transport 74 NM_001099222.2 61.2% 59.1% (many diffs)
4 human 80173 IFT74 intraflagellar transport 74 NM_001099223.2 61.2% 59.1% (many diffs)
5 human 80173 IFT74 intraflagellar transport 74 NM_025103.4 61.2% 59.1% (many diffs)
6 human 80173 IFT74 intraflagellar transport 74 XM_017015164.1 26.1% 23.8% (many diffs)
7 human 80173 IFT74 intraflagellar transport 74 XM_011518036.2 24.4% 22.3% (many diffs)
8 human 80173 IFT74 intraflagellar transport 74 XM_017015163.1 24.4% 22.3% (many diffs)
9 mouse 67694 Ift74 intraflagellar transport 74 NM_001290568.1 83.7% 81.6% (many diffs)
10 mouse 67694 Ift74 intraflagellar transport 74 XM_011240598.2 57.2% 57.1% (many diffs)
11 mouse 67694 Ift74 intraflagellar transport 74 NM_026319.3 53.9% 53.8% (many diffs)
12 mouse 67694 Ift74 intraflagellar transport 74 XM_006503324.3 53.9% 53.8% (many diffs)
13 mouse 67694 Ift74 intraflagellar transport 74 XM_006503326.2 53.9% 53.8% (many diffs)
14 mouse 67694 Ift74 intraflagellar transport 74 XM_011240597.2 53.9% 53.8% (many diffs)
15 mouse 67694 Ift74 intraflagellar transport 74 XM_006503327.3 49.6% 48.2% (many diffs)
16 mouse 67694 Ift74 intraflagellar transport 74 XM_006503328.3 49.6% 49.1% (many diffs)
17 mouse 67694 Ift74 intraflagellar transport 74 XR_001784189.1 42.1% (many diffs)
18 mouse 67694 Ift74 intraflagellar transport 74 XM_006503329.2 36.4% 36% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1185
ORF length:
1116
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCCAGCAAT CACAAATCTT CAGCAGCTCG CCCTGTTTCA AGAGGTGGAG
121TTGGGTTAAC AGGAAGGCCT CCTTCTGGGA TACGACCCCT ATCAGGAAAT ATTCGAGTGG
181CAACTGCAAT GCCACCTGGG ACAGCAAGAC CAGGTTCTCG TGGTTGTCCC ATAGGGACTG
241GTGGAGTTCT GTCTTCTCAA ATCAAAGTTG CCCATCGCCC TGTAACACAA CAAGGTTTGA
301CTGGAATGAA AACTGGGACG AAAGGTCCCC AGAGGCAAAT TTTAGACAAA TCTTACTATC
361TTGGGCTTCT TAGAAGTAAA ATAAGTGAAC TTACAACTGA AGTTAATAAA CTTCAGAAGG
421GAATAGAAAT GTACAATCAA GAGAATTCAG TATATTTGTC ATATGAAAAG AGGGCTGAGA
481CTTTAGCTGT TGAGATAAAA GAGCTTCAAG GACAACTAGC AGACTACAAC ATGTTGGTAG
541ATAAACTTAA TACCAACACT GAAATGGAAG AAGTAATGAA TGATTACAAT ATGCTTAAAG
601CTCAAAATGA TCGAGAAACA CAAAGTTTGG ATGTCATATT TACTGAAAGA CAAGCGAAAG
661AAAAACAAAT CAGAAGTGTC GAAGAAGAAA TTGAACAGGA AAAACAAGCA ACAGATGACA
721TTATCAAAAA TATGTCTTTT GAAAACCAAG TCAAGTACCT AGAGATGAAA ACCACAAATG
781AGAAACTGTT ACAGGAATTA GATACACTTC AACAACAATT GGATTCACAG AACATGAAAA
841AAGAGAGCCT GGAAGCAGAA ATAGCTCACT CCCAGGTGAA ACAGGAGGCG GTATTGCTGC
901ATGAAAAACT TTATGAGTTG GAGTCCCATC GAGATCAAAT GATTGCAGAA GACAAAAGCA
961TAGGATCTCC AATGGAAGAG AGAGAGAAAT TACTTAAGCA GATTAAAGAT GATAATCAGG
1021AAATAGCCAG CATGGAAAGA CAGTTAACAG ATACAAAAGA AAAGATAAAT CAGTTTATTG
1081AAGAAATTAG ACAACTTGAC ATGGATTTAG AGGAACACCA AGATCCTACC AACTATGGAT
1141GGAAAATACT TGAAAAAAAT ACAGGAAGTT TCAAAAAGCA AGTTTTGCCA ACTTTCTTGT
1201ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)