Construct: ORF ccsbBroadEn_04573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008365.1_s300c1, BRDN0000388660
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS36 (92259)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92259 | MRPS36 | mitochondrial ribosomal pro... | NM_033281.6 | 100% | 100% | |
| 2 | mouse | 66128 | Mrps36 | mitochondrial ribosomal pro... | NM_025369.3 | 84.7% | 83.4% | (many diffs) |
| 3 | mouse | 66128 | Mrps36 | mitochondrial ribosomal pro... | NM_001190264.1 | 83.4% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 375
- ORF length:
- 309
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAT GGGCAGCAAG ATGGCGTCTG CTAGTAGGGT CGTTCAGGTA GTCAAACCAC 121 ACACTCCATT AATAAGGTTT CCTGACAGAA GAGACAATCC TAAACCCAAT GTATCAGAAG 181 CTTTGAGATC AGCAGGGCTA CCATCTCACT CTTCTGTAAT TTCACAACAT TCTAAAGGAA 241 GTAAATCACC AGATTTGCTG ATGTATCAGG GTCCACCAGA CACTGCAGAA ATAATAAAAA 301 CATTACCTCA GAAATACAGA AGGAAACTTG TGTCTCAAGA AGAAATGGAA TTTATCCAAC 361 GTGGAGGTCC TGAATACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 421 tatcaatttg ttgcaacgaa c