Construct: ORF ccsbBroadEn_05186

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000798.1_s300c1, BRDN0000391599
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APOBEC3A (200315)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100913187 APOBEC3A_B APOBEC3A and APOBEC3B delet... NM_001193289.1 100% 100%
2 human 200315 APOBEC3A apolipoprotein B mRNA editi... NM_145699.4 100% 100%
3 human 200315 APOBEC3A apolipoprotein B mRNA editi... NM_001270406.2 90.9% 90.9% 27_28ins54
4 human 9582 APOBEC3B apolipoprotein B mRNA editi... NM_004900.5 48.5% 44.7% (many diffs)
5 human 9582 APOBEC3B apolipoprotein B mRNA editi... NM_001270411.2 41.9% 38.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
666
ORF length:
597
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGAAGCCAGC CCAGCATCCG GGCCCAGACA CTTGATGGAT CCACACATAT
121TCACTTCCAA CTTTAACAAT GGCATTGGAA GGCATAAGAC CTACCTGTGC TACGAAGTGG
181AGCGCCTGGA CAATGGCACC TCGGTCAAGA TGGACCAGCA CAGGGGCTTT CTACACAACC
241AGGCTAAGAA TCTTCTCTGT GGCTTTTACG GCCGCCATGC GGAGCTGCGC TTCTTGGACC
301TGGTTCCTTC TTTGCAGTTG GACCCGGCCC AGATCTACAG GGTCACTTGG TTCATCTCCT
361GGAGCCCCTG CTTCTCCTGG GGCTGTGCCG GGGAAGTGCG TGCGTTCCTT CAGGAGAACA
421CACACGTGAG ACTGCGTATC TTCGCTGCCC GCATCTATGA TTACGACCCC CTATATAAGG
481AGGCACTGCA AATGCTGCGG GATGCTGGGG CCCAAGTCTC CATCATGACC TACGATGAAT
541TTAAGCACTG CTGGGACACC TTTGTGGACC ACCAGGGATG TCCCTTCCAG CCCTGGGATG
601GACTAGATGA GCACAGCCAA GCCCTGAGTG GGAGGCTGCG GGCCATTCTC CAGAATCAGG
661GAAACTTGCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
721gttgcaacga ac

Download FASTA (ORF) (Full)