Construct: ORF ccsbBroadEn_05190

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006635.1_s300c1, BRDN0000386370
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIKFYVE (200576)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 200576 PIKFYVE phosphoinositide kinase, FY... NM_152671.3 100% 100%
2 human 200576 PIKFYVE phosphoinositide kinase, FY... NM_001178000.1 82.2% 82.2% 321_611del
3 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510792.3 33.6% 33.5% (many diffs)
4 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510788.1 22.5% 22.4% 1346delA;1350_1365del;1371_6003del
5 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510787.1 22.4% 22.3% (many diffs)
6 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510786.3 22.4% 22.3% (many diffs)
7 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003570.1 22% 20.6% (many diffs)
8 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510782.3 21.9% 21.8% (many diffs)
9 human 200576 PIKFYVE phosphoinositide kinase, FY... NM_015040.4 21.4% 21.4% (many diffs)
10 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510780.2 21.3% 21.2% (many diffs)
11 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510778.3 21.3% 21.2% (many diffs)
12 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510779.2 21.3% 21.2% (many diffs)
13 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003568.1 21.2% 21% (many diffs)
14 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510781.3 21% 20.9% (many diffs)
15 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510784.2 20.8% 19.5% (many diffs)
16 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510783.3 20.8% 19.5% (many diffs)
17 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003569.1 20.8% 19.3% (many diffs)
18 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510785.3 20.6% 19.2% (many diffs)
19 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510789.2 20% 19.9% (many diffs)
20 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003571.1 19.4% 18% (many diffs)
21 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_011510790.1 11.4% 11.3% (many diffs)
22 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003572.1 11.4% 11.3% (many diffs)
23 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003573.1 11.4% 11.3% (many diffs)
24 human 200576 PIKFYVE phosphoinositide kinase, FY... XM_017003574.1 11.4% 11.3% (many diffs)
25 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_011238454.2 29.5% 30.6% (many diffs)
26 mouse 18711 Pikfyve phosphoinositide kinase, FY... NM_001310624.1 19.7% 20.5% (many diffs)
27 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_006495781.3 19.7% 20.5% (many diffs)
28 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_006495779.3 19.6% 20.3% (many diffs)
29 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_006495776.3 19.6% 20.3% (many diffs)
30 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_006495777.3 19.6% 20.3% (many diffs)
31 mouse 18711 Pikfyve phosphoinositide kinase, FY... NM_011086.2 19.3% 18.5% (many diffs)
32 mouse 18711 Pikfyve phosphoinositide kinase, FY... XM_006495782.3 19.3% 18.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1419
ORF length:
1353
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CACAGATGAT AAGACGTCCC CAACACTGGA CTCTGCTAAT GATTTGCCTC
121GATCTCCTAC TAGTCCTTCT CATCTCACAC ACTTTAAACC TTTGACTCCT GATCAAGATG
181AGCCCCCTTT TAAATCAGCT TATAGTTCTT TTGTAAATCT CTTTCGTTTT AACAAAGAGA
241GAGCAGAAGG AGGCCAGGGA GAACAGCAGC CTTTGAGTGG AAGTTGGACC AGCCCTCAGC
301TCCCTTCGAG GACACAGTCT GTTAGGTCAC CCACACCTTA TAAAAAGCAG CTTAATGAGG
361AACTCCAGCG GCGCTCTTCA GCATTAGGAG ACCTCCGAGC TTGCACATAT TGTAGAAAAA
421TAGCCTTAAG TTATGCTCAT TCCACAGACA GTAATTCTAT TGGGGAAGAC TTGAATGCTC
481TTTCAGATTC TGCTTGCTCT GTGTCTGTGC TTGATCCAAG TGAACCCCGA ACACCTGTTG
541GGAGTAGGAA AGCCAGCCGT AACATATTTT TAGAGGATGA TTTGGCCTGG CAAAGTTTGA
601TTCATCCAGA TTCCTCAAAT ACTCCTCTTT CAACAAGACT TGTATCTGTG CAAGAGGATG
661CTGGGAAATC TCCTGCTCGA AATAGATCAG CCAGCATTAC TAACCTGTCA CTGGATAGAT
721CTGGTTCTCC TATGGTACCT TCATATGAGA CATCTGTCAG TCCCCAGGCT AACCGAACAT
781ATGTTAGGAC AGAGACCACT GAGGATGAAC GCAAAATTCT TCTGGACAGT GTGCAGTTAA
841AAGACCTGTG GAAAAAAATC TGCCATCACA GCAGTGGAAT GGAGTTTCAG GATCACCGCT
901ACTGGTTGAG AACGCATCCC AACTGCATTG TAGGAAAGGA ATTAGTCAAC TGGCTAATCC
961GAAATGGGCA TATTGCCACA AGGGCACAAG CTATAGCAAT TGGACAAGCA ATGGTTGATG
1021GACGTTGGCT GGATTGTGTT AGTCATCACG ACCAGCTTTT CAGAGATGAG TATGCGCTGT
1081ATAGACCACT GCAGAGTACA GAATTTTCTG AGACGCCTTC TCCCGACAGT GACTCAGTGA
1141ACTCCGTGGA AGGACACTCT GAGCCATCCT GGTTTAAAGA CATAAAGTTT GATGACAGTG
1201ACACAGAACA GATAGCTGAA GAAGGTGACG ATAATTTGGC TAATTCTGCC AGTCCTAGCA
1261AGCGCACATC AGTCAGCAGT TTCCAGTCCA CAGTGGACAG TGACTCAGCC GCTTCTATCA
1321GCCTGAACGT GGAGCTGGAC AACGTGAACT TCCATATCAA GAAGCCCTCC AAGTACCCAC
1381ATGTGCCCCC TCACCCTGCT GACCAAAAAG GTAGGAGGTA CCCAACTTTC TTGTACAAAG
1441Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)