Construct: ORF ccsbBroadEn_05520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015231.1_s300c1, BRDN0000394561
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE1B (353132)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353132 | LCE1B | late cornified envelope 1B | NM_178349.2 | 100% | 100% | |
| 2 | human | 353133 | LCE1C | late cornified envelope 1C | NM_178351.4 | 96% | 96.6% | (many diffs) |
| 3 | human | 353135 | LCE1E | late cornified envelope 1E | NM_178353.2 | 93.7% | 93.2% | (many diffs) |
| 4 | human | 353137 | LCE1F | late cornified envelope 1F | NM_178354.2 | 89.4% | 90.6% | (many diffs) |
| 5 | human | 353134 | LCE1D | late cornified envelope 1D | NM_178352.3 | 88.9% | 86.5% | (many diffs) |
| 6 | human | 353131 | LCE1A | late cornified envelope 1A | NM_178348.2 | 87.2% | 85.5% | (many diffs) |
| 7 | human | 353133 | LCE1C | late cornified envelope 1C | NM_001276331.2 | 72.8% | 72.8% | (many diffs) |
| 8 | human | 199834 | LCE4A | late cornified envelope 4A | NM_178356.3 | 62.5% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GTCCTGCCAG CAGAACCAGC AGCAGTGCCA GCCCCCTCCC AAGTGCATCC 121 CCAAGTGCCC TCCCAAGTGC CTCACCCCTA GATGCCCCCC AAAGTGTCCC CCTAAGTGTC 181 CTCCAGTCTC TTCCTGCTGC AGTGTCAGCT CCGGAGGCTG CTGTGGCTCC AGCTCTGGGG 241 GAAGCTGTGG CTCCAGCTCT GGGGGTTGCT GCAGTTCTGG GGGAGGTGGC TGCTGCCTGA 301 GCCACCACAG GCGCCGTAGG TCCCACTGCC ACAGACCCCA GAGCTCTGGC TGCTGCAGCC 361 AGCCCTCCGG GGGCTCCAGC TGCTGTGGAG GAGGGAGTGG CCAGCACTCT GGAGGCTGCT 421 GCTTGCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt 481 gcaacgaac