Construct: ORF ccsbBroadEn_05634

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005885.1_s300c1, BRDN0000455292
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRAPL (400581)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 400581 GRAPL GRB2 related adaptor protei... NM_001129778.3 100% 100%
2 human 400581 GRAPL GRB2 related adaptor protei... XM_017024637.1 100% 100%
3 human 400581 GRAPL GRB2 related adaptor protei... NM_001353418.2 98.3% 97.4% 299_300insTAACAG
4 human 400581 GRAPL GRB2 related adaptor protei... XM_011523857.3 86.7% 83.3% (many diffs)
5 human 400581 GRAPL GRB2 related adaptor protei... XM_017024635.2 77.4% 71% (many diffs)
6 human 400581 GRAPL GRB2 related adaptor protei... XM_017024636.2 77.4% 71% (many diffs)
7 human 10750 GRAP GRB2 related adaptor protein NM_001330148.2 53.6% 50.7% (many diffs)
8 human 10750 GRAP GRB2 related adaptor protein NM_006613.4 48.5% 47% (many diffs)
9 mouse 71520 Grap GRB2-related adaptor protein NM_027817.3 46.1% 45.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
423
ORF length:
354
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGAGTCCGTG GCCCTGTACA GCTTTCAGGC TACAGAGAGC GACGAGCTGG
121CCTTCAACAA GGGAGACACA CTCAAGATCC TGAACATGGA GGATGACCAG AACTGGTACA
181AGGCCGAGCT CCGGGGTGTC GAGGGATTTA TTCCCAAGAA CTACATCCGC GTCAAGCCCC
241ATCCGTGGTA CTCGGGCAGG ATTTCCCGGC AGCTGGCCGA AGAGATTCTG ATGAAGCGGA
301ACCATCTGGG AGCCTTCCTG ATCCGGGAGA GTGAGAGCTC CCCAGGGGAG TTCTCTGTGT
361CTGTGAATAA CAGAGCCCAG AGGGGTCCCT GCCTGGGCCC AAAATCACAC AGCAGATTGG
421GGTTGCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt
481gcaacgaac

Download FASTA (ORF) (Full)