Transcript: Human NM_006613.4

Homo sapiens GRB2 related adaptor protein (GRAP), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
GRAP (10750)
Length:
1988
CDS:
32..685

Additional Resources:

NCBI RefSeq record:
NM_006613.4
NBCI Gene record:
GRAP (10750)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006613.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415694 ACTGAACGTGGGCTCCTAACT pLKO_005 800 3UTR 100% 4.950 3.960 N GRAP n/a
2 TRCN0000107165 GCCCTGGCTCTTGTTTGTTTA pLKO.1 1406 3UTR 100% 13.200 9.240 N GRAP n/a
3 TRCN0000423660 TGGAGTTGTTCAGGCTGTTGG pLKO_005 1126 3UTR 100% 4.050 2.835 N GRAP n/a
4 TRCN0000425812 TTTCACCCACTGGAGGCTCAA pLKO_005 1021 3UTR 100% 4.050 2.835 N GRAP n/a
5 TRCN0000107168 GTCTGTGAACTATGGAGACCA pLKO.1 322 CDS 100% 2.640 1.848 N GRAP n/a
6 TRCN0000107167 CATCGCCAAGAAGCGGCAGAT pLKO.1 448 CDS 100% 1.350 0.945 N GRAP n/a
7 TRCN0000107169 AGACACACTCAAGATCCTGAA pLKO.1 97 CDS 100% 4.050 2.025 Y GRAP n/a
8 TRCN0000107166 CAAGAACTACATCCGCGTCAA pLKO.1 178 CDS 100% 4.050 2.025 Y GRAP n/a
9 TRCN0000097066 GATGACCAGAACTGGTACAAA pLKO.1 125 CDS 100% 5.625 2.813 Y Grap n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006613.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15726 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15726 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_05634 pDONR223 100% 48.5% 47% None (many diffs) n/a
4 ccsbBroad304_05634 pLX_304 0% 48.5% 47% V5 (many diffs) n/a
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