Transcript: Human XM_011523857.3

PREDICTED: Homo sapiens GRB2 related adaptor protein like (GRAPL), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRAPL (400581)
Length:
4785
CDS:
228..539

Additional Resources:

NCBI RefSeq record:
XM_011523857.3
NBCI Gene record:
GRAPL (400581)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011523857.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 4675 3UTR 100% 13.200 6.600 Y LRRC74B n/a
2 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2750 3UTR 100% 4.950 2.475 Y CFLAR n/a
3 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2750 3UTR 100% 4.950 2.475 Y C19orf31 n/a
4 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2828 3UTR 100% 4.950 2.475 Y ORAI2 n/a
5 TRCN0000107169 AGACACACTCAAGATCCTGAA pLKO.1 293 CDS 100% 4.050 2.025 Y GRAP n/a
6 TRCN0000107166 CAAGAACTACATCCGCGTCAA pLKO.1 374 CDS 100% 4.050 2.025 Y GRAP n/a
7 TRCN0000097066 GATGACCAGAACTGGTACAAA pLKO.1 321 CDS 100% 5.625 2.813 Y Grap n/a
8 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2825 3UTR 100% 4.950 2.475 Y LOC339059 n/a
9 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2748 3UTR 100% 4.950 2.475 Y ERN2 n/a
10 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2748 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2748 3UTR 100% 4.950 2.475 Y P3H4 n/a
12 TRCN0000172742 GAGACAGAGTCTTGCTCTGTT pLKO.1 4447 3UTR 100% 0.495 0.248 Y C11orf44 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011523857.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05634 pDONR223 100% 86.7% 83.3% None (many diffs) n/a
2 ccsbBroad304_05634 pLX_304 0% 86.7% 83.3% V5 (many diffs) n/a
3 ccsbBroadEn_15726 pDONR223 0% 46.8% 45.6% None (many diffs) n/a
4 ccsbBroad304_15726 pLX_304 0% 46.8% 45.6% V5 (many diffs) n/a
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