Construct: ORF ccsbBroadEn_05830

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF010880.1_s300c1, BRDN0000456801
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: APBA2 (321)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	321	APBA2	amyloid beta precursor prot...	NM_001130414.1	99.9%	99.8%	932T>C;1773C>T
2	human	321	APBA2	amyloid beta precursor prot...	NM_001353792.2	99.9%	99.8%	932T>C;1773C>T
3	human	321	APBA2	amyloid beta precursor prot...	NM_001353793.2	99.9%	99.8%	932T>C;1773C>T
4	human	321	APBA2	amyloid beta precursor prot...	NM_001353794.2	99.9%	99.8%	932T>C;1773C>T
5	human	321	APBA2	amyloid beta precursor prot...	XM_024449909.1	99.6%	99.5%	932T>C;1214_1219delAGGTAG;1779C>T
6	human	321	APBA2	amyloid beta precursor prot...	NM_001353788.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
7	human	321	APBA2	amyloid beta precursor prot...	NM_001353789.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
8	human	321	APBA2	amyloid beta precursor prot...	NM_001353790.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
9	human	321	APBA2	amyloid beta precursor prot...	NM_001353791.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
10	human	321	APBA2	amyloid beta precursor prot...	NM_005503.3	98.3%	98.2%	932T>C;1215_1250del;1809C>T
11	human	321	APBA2	amyloid beta precursor prot...	XM_011521488.3	98.3%	98.2%	932T>C;1215_1250del;1809C>T
12	human	321	APBA2	amyloid beta precursor prot...	XM_011521489.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
13	human	321	APBA2	amyloid beta precursor prot...	XM_011521490.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
14	human	321	APBA2	amyloid beta precursor prot...	XM_011521491.1	98.3%	98.2%	932T>C;1215_1250del;1809C>T
15	human	321	APBA2	amyloid beta precursor prot...	XM_011521492.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
16	human	321	APBA2	amyloid beta precursor prot...	XM_017022110.1	98.3%	98.2%	932T>C;1215_1250del;1809C>T
17	human	321	APBA2	amyloid beta precursor prot...	XM_017022112.2	98.3%	98.2%	932T>C;1215_1250del;1809C>T
18	human	321	APBA2	amyloid beta precursor prot...	NM_001353795.2	90.7%	89.2%	(many diffs)
19	human	321	APBA2	amyloid beta precursor prot...	NM_001353797.2	58.8%	57.6%	(many diffs)
20	human	321	APBA2	amyloid beta precursor prot...	NM_001353796.2	57.9%	56.7%	(many diffs)
21	mouse	11784	Apba2	amyloid beta (A4) precursor...	XM_006540576.3	87.6%	92.4%	(many diffs)
22	mouse	11784	Apba2	amyloid beta (A4) precursor...	NM_001291166.1	86.2%	90.9%	(many diffs)
23	mouse	11784	Apba2	amyloid beta (A4) precursor...	NM_001291167.1	86.2%	90.9%	(many diffs)
24	mouse	11784	Apba2	amyloid beta (A4) precursor...	NM_007461.2	86.2%	90.9%	(many diffs)
25	mouse	11784	Apba2	amyloid beta (A4) precursor...	XM_006540573.3	86.2%	90.9%	(many diffs)
26	mouse	11784	Apba2	amyloid beta (A4) precursor...	XM_006540574.3	86.2%	90.9%	(many diffs)
27	mouse	11784	Apba2	amyloid beta (A4) precursor...	XM_017321945.1	86.2%	90.9%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

2280

ORF length:

2211

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61	TTGGCACCAT GGCCCACCGG AAGCTTGAGA GCGTGGGGAG CGGCATGTTG GACCATAGGG
121	TGAGACCAGG TCCTGTCCCT CACAGCCAGG AGCCCGAGAG CGAGGACATG GAGCTGCCCT
181	TGGAGGGCTA TGTGCCCGAG GGCCTGGAGC TGGCTGCCCT GCGGCCAGAG AGCCCCGCGC
241	CAGAGGAACA GGAGTGCCAC AACCACAGCC CCGATGGGGA CTCCAGCTCT GACTACGTGA
301	ACAACACCTC TGAGGAGGAG GACTATGACG AGGGCCTCCC TGAGGAGGAG GAGGGCATCA
361	CCTACTACAT CCGCTACTGC CCTGAGGACG ACAGCTACCT AGAGGGCATG GACTGCAACG
421	GGGAGGAGTA CCTGGCCCAC AGTGCACACC CTGTGGACAC TGATGAGTGC CAGGAGGCGG
481	TGGAGGAGTG GACGGACTCG GCGGGCCCGC ACCCCCACGG CCACGAGGCT GAAGGCAGCC
541	AGGACTACCC AGACGGCCAA CTGCCCATTC CGGAGGATGA GCCCTCCGTC CTTGAGGCCC
601	ATGACCAGGA AGAAGATGGT CACTACTGTG CCAGCAAAGA GGGCTACCAG GACTACTACC
661	CCGAGGAGGC CAACGGGAAC ACCGGCGCCT CCCCCTACCG CCTGAGGCGT GGGGATGGGG
721	ACCTGGAGGA CCAGGAGGAG GACATTGACC AGATCGTGGC AGAGATCAAG ATGAGTCTGA
781	GCATGACCAG CATCACCAGC GCCAGTGAGG CCAGCCCCGA GCATGGGCCT GAGCCAGGGC
841	CTGAGGACTC TGTAGAGGCC TGCCCACCCA TCAAGGCCAG CTGCAGCCCC AGCAGGCACG
901	AGGCGAGGCC CAAGTCGCTG AACCTCCTTC CCGAGGCCAA GCACCCCGGA GACCCCCAGA
961	GAGGCTTCAA GCCCAAGACC AGGACCCCAG AAGAGAGGCC GAAGTGGCCC CACGAGCAGG
1021	TTTGCAATGG TCTGGAGCAG CCAAGGAAGC AGCAGCGCTC TGATCTCAAT GGACCTGTTG
1081	ACAATAACAA CATTCCAGAG ACAAAGAAGG TGGCATCATT TCCAAGTTTT GTGGCTGTTC
1141	CAGGGCCCTG CGAACCAGAA GACCTCATCG ACGGGATCAT CTTTGCTGCC AATTACCTGG
1201	GGTCCACCCA GCTGCTATCA GAACGGAACC CTTCCAAAAA CATCAGAATG ATGCAAGCGC
1261	AGGAGGCCGT CAGCCGGGTC AAGAATTCTG AGGGGGATGC CCAGACGCTG ACGGAAGTGG
1321	ACCTCTTCAT TTCCACCCAG AGGATCAAGG TTTTAAATGC AGACACGCAG GAAACCATGA
1381	TGGACCACGC CTTGCGTACC ATCTCCTACA TCGCCGACAT TGGGAACATT GTAGTGCTGA
1441	TGGCCAGACG CCGCATGCCC CGGTCAGCCT CTCAGGACTG CATCGAGACC ACGCCCGGGG
1501	CCCAGGAAGG CAAGAAGCAG TATAAGATGA TCTGCCATGT GTTCGAGTCG GAGGATGCCC
1561	AGCTCATCGC CCAGTCTATC GGCCAGGCCT TCAGCGTGGC CTACCAGGAG TTCCTGCGAG
1621	CCAATGGCAT CAACCCCGAA GACTTGAGCC AGAAGGAATA CAGCGACATC ATCAACACCC
1681	AGGAGATGTA CAACGACGAC CTCATCCACT TCTCAAACTC GGAGAACTGC AAGGAGCTGC
1741	AGCTGGAGAA GCACAAGGGC GAGATCCTGG GCGTGGTGGT GGTGGAGTCG GGCTGGGGCT
1801	CCATCCTGCC CACGGTGATC CTGGCCAACA TGATGAATGG TGGCCCGGCT GCCCGCTCGG
1861	GGAAGCTGAG CATCGGGGAC CAGATCATGT CCATCAATGG CACCAGCCTG GTGGGGCTGC
1921	CCCTCGCCAC CTGCCAAGGC ATCATCAAGG GCCTGAAGAA CCAGACACAG GTGAAGCTCA
1981	ACATTGTCAG CTGTCCCCCG GTCACCACGG TCCTTATCAA GCGGCCAGAC CTCAAGTACC
2041	AGCTGGGCTT CAGCGTGCAG AATGGAATTA TCTGCAGCCT CATGAGAGGG GGCATTGCTG
2101	AGCGAGGGGG CGTCCGTGTG GGCCACCGCA TCATCGAGAT CAACGGGCAG AGCGTGGTGG
2161	CCACAGCCCA CGAGAAGATA GTCCAAGCTC TGTCCAACTC GGTCGGAGAG ATCCACATGA
2221	AGACCATGCC CGCCGCCATG TTCAGGCTCC TCACGGGTCA GGAGACCCCG CTGTACATCT
2281	TGCCAACTTT CTTGTACAAA GTTGGcatta taagaaagca ttgcttatca atttgttgca
2341	acgaac

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