Construct: ORF ccsbBroadEn_05924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006504.1_s300c1, BRDN0000457038
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNB2 (783)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201596.3 | 99.9% | 99.8% | 1823G>A |
| 2 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201571.4 | 95% | 93.9% | (many diffs) |
| 3 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201597.3 | 94.8% | 93.5% | (many diffs) |
| 4 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201593.3 | 94% | 93.6% | (many diffs) |
| 5 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201570.3 | 91.2% | 90.3% | (many diffs) |
| 6 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_000724.4 | 90.9% | 90% | (many diffs) |
| 7 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201590.3 | 90.6% | 88.5% | (many diffs) |
| 8 | human | 783 | CACNB2 | calcium voltage-gated chann... | XM_006717502.3 | 90.1% | 89.5% | (many diffs) |
| 9 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_201572.4 | 89.8% | 87.6% | (many diffs) |
| 10 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_001167945.2 | 89.1% | 87.7% | (many diffs) |
| 11 | human | 783 | CACNB2 | calcium voltage-gated chann... | XM_011519659.2 | 85.5% | 82.3% | (many diffs) |
| 12 | human | 783 | CACNB2 | calcium voltage-gated chann... | XM_005252588.4 | 85.3% | 84% | (many diffs) |
| 13 | human | 783 | CACNB2 | calcium voltage-gated chann... | NM_001330060.2 | 85% | 83.7% | (many diffs) |
| 14 | human | 783 | CACNB2 | calcium voltage-gated chann... | XM_005252591.3 | 57.5% | 57.4% | 0_1ins840;983G>A |
| 15 | human | 783 | CACNB2 | calcium voltage-gated chann... | XM_017016625.1 | 57.5% | 57.4% | 0_1ins840;983G>A |
| 16 | human | 783 | CACNB2 | calcium voltage-gated chann... | XR_001747198.1 | 22.7% | (many diffs) | |
| 17 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497313.3 | 89.8% | 93.6% | (many diffs) |
| 18 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | NM_023116.4 | 89.6% | 93.4% | (many diffs) |
| 19 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497315.3 | 86% | 89.3% | (many diffs) |
| 20 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497314.3 | 85.8% | 89.2% | (many diffs) |
| 21 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497318.3 | 84% | 87.4% | (many diffs) |
| 22 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497317.3 | 83.8% | 87.2% | (many diffs) |
| 23 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497319.3 | 82.6% | 85.6% | (many diffs) |
| 24 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | NM_001309519.1 | 82.4% | 85.4% | (many diffs) |
| 25 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497320.1 | 82.1% | 85.1% | (many diffs) |
| 26 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | NM_001252533.1 | 82.1% | 84.8% | (many diffs) |
| 27 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497321.3 | 80.2% | 83.1% | (many diffs) |
| 28 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_011238946.2 | 76.3% | 78.9% | (many diffs) |
| 29 | mouse | 12296 | Cacnb2 | calcium channel, voltage-de... | XM_006497323.1 | 71.6% | 74.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2049
- ORF length:
- 1980
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGTCCAAAGG GACATGTCCA AGTCGCCTCC CACAGCGGCG GCGGCGGTGG 121 CGCAGGAGAT CCAGATGGAA CTGCTAGAGA ACGTGGCTCC CGCGGGGGCG CTCGGAGCCG 181 CCGCACAGTC ATATGGAAAA GGAGCCAGAA GGAAAAACAG ATTTAAAGGA TCTGATGGAA 241 GCACGTCATC TGATACTACC TCAAATAGTT TTGTTCGCCA GGGTTCGGCA GACTCCTACA 301 CTAGCCGTCC ATCCGATTCC GATGTATCTC TGGAGGAGGA CCGGGAGGCA GTGCGCAGAG 361 AAGCGGAGCG GCAGGCCCAG GCACAGTTGG AAAAAGCAAA GACAAAGCCC GTTGCATTTG 421 CGGTTCGGAC AAATGTCAGC TACAGTGCGG CCCATGAAGA TGATGTTCCA GTGCCTGGCA 481 TGGCCATCTC ATTCGAAGCA AAAGATTTTC TGCATGTTAA GGAAAAATTT AACAATGACT 541 GGTGGATAGG GCGATTGGTA AAAGAAGGCT GTGAAATCGG ATTCATTCCA AGCCCAGTCA 601 AACTAGAAAA CATGAGGCTG CAGCATGAAC AGAGAGCCAA GCAAGGGAAA TTCTACTCCA 661 GTAAATCAGG AGGAAATTCA TCATCCAGTT TGGGTGACAT AGTACCTAGT TCCAGAAAAT 721 CAACACCTCC ATCATCTGCT ATAGACATAG ATGCTACTGG CTTAGATGCA GAAGAAAATG 781 ATATTCCAGC AAACCACCGC TCCCCTAAAC CCAGTGCAAA CAGTGTAACG TCACCCCACT 841 CCAAAGAGAA AAGAATGCCC TTCTTTAAGA AGACAGAGCA CACTCCTCCG TATGATGTGG 901 TACCTTCCAT GCGACCAGTG GTCCTAGTGG GCCCTTCTCT GAAGGGCTAC GAGGTCACAG 961 ATATGATGCA AAAAGCGCTG TTTGATTTTT TAAAACACAG ATTTGAAGGG CGGATATCCA 1021 TCACAAGGGT CACCGCTGAC ATCTCGCTTG CCAAACGCTC GGTATTAAAC AATCCCAGTA 1081 AGCACGCAAT AATAGAAAGA TCCAACACAA GGTCAAGCTT AGCGGAAGTT CAGAGTGAAA 1141 TCGAAAGGAT TTTTGAACTT GCAAGAACAT TGCAGTTGGT GGTCCTTGAC GCGGATACAA 1201 TTAATCATCC AGCTCAACTC AGTAAAACCT CCTTGGCCCC TATTATAGTA TATGTAAAGA 1261 TTTCTTCTCC TAAGGTTTTA CAAAGGTTAA TAAAATCTCG AGGGAAATCT CAAGCTAAAC 1321 ACCTCAACGT CCAGATGGTA GCAGCTGATA AACTGGCTCA GTGTCCTCCA GAGCTGTTCG 1381 ATGTGATCTT GGATGAGAAC CAGCTTGAGG ATGCCTGTGA GCACCTTGCC GACTATCTGG 1441 AGGCCTACTG GAAGGCCACC CATCCTCCCA GCAGTAGCCT CCCCAACCCT CTCCTTAGCC 1501 GTACATTAGC CACTTCAAGT CTGCCTCTTA GCCCCACCCT AGCCTCTAAT TCACAGGGTT 1561 CTCAAGGTGA TCAGAGGACT GATCGCTCCG CTCCTATCCG TTCTGCTTCC CAAGCTGAAG 1621 AAGAACCTAG TGTGGAACCA GTCAAGAAAT CCCAGCACCG CTCTTCCTCC TCAGCCCCAC 1681 ACCACAACCA TCGCAGTGGG ACAAGTCGCG GCCTCTCCAG GCAAGAGACA TTTGACTCGG 1741 AAACCCAGGA GAGTCGAGAC TCTGCCTACG TAGAGCCAAA GGAAGATTAT TCCCATGACC 1801 ACGTGGACCA CTATGCCTCA CACCGTGACC ACAACCACAG AGACGAGACC CACGGGAGCA 1861 GTGACCACAG ACACAGGGAG TCCCGGCACC ATTCCCGGGA CGTGGATCGA GAGCAGGACC 1921 ACAACGAGTG CAACAAGCAG CGCAGCCGTC ATAAATCCAA GGATCGCTAC TGTGAAAAGG 1981 ATGGAGAAGT GATATCAAAA AAACGGAATG AGGCTGGGGA GTGGAACAGG GATGTTTACA 2041 TCCGCCAATT GCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa 2101 tttgttgcaa cgaac