Transcript: Human XM_006717502.3

PREDICTED: Homo sapiens calcium voltage-gated channel auxiliary subunit beta 2 (CACNB2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CACNB2 (783)
Length:
8253
CDS:
294..2096

Additional Resources:

NCBI RefSeq record:
XM_006717502.3
NBCI Gene record:
CACNB2 (783)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006717502.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422285 AGCAGCGCAGCCGTCATAAAT pLKO_005 1981 CDS 100% 15.000 21.000 N CACNB2 n/a
2 TRCN0000043873 GCCGTACATTAGCCACTTCAA pLKO.1 1543 CDS 100% 4.950 6.930 N CACNB2 n/a
3 TRCN0000043874 CGGTATTAAACAATCCCAGTA pLKO.1 1105 CDS 100% 4.050 5.670 N CACNB2 n/a
4 TRCN0000043875 CGAGGGAAATCTCAAGCTAAA pLKO.1 1344 CDS 100% 10.800 8.640 N CACNB2 n/a
5 TRCN0000413535 GTGGATAGGGCGATTGGTAAA pLKO_005 587 CDS 100% 10.800 8.640 N CACNB2 n/a
6 TRCN0000043877 CGCAATAATAGAAAGATCCAA pLKO.1 1130 CDS 100% 3.000 2.400 N CACNB2 n/a
7 TRCN0000043876 CCAGTAAATCAGGAGGAAATT pLKO.1 703 CDS 100% 13.200 9.240 N CACNB2 n/a
8 TRCN0000422085 ACAGATTTGAAGGGCGGATAT pLKO_005 1042 CDS 100% 10.800 7.560 N CACNB2 n/a
9 TRCN0000069107 CCCAAGCTGAAGAAGAACCTT pLKO.1 1654 CDS 100% 3.000 2.100 N Cacnb2 n/a
10 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 4137 3UTR 100% 10.800 5.400 Y SMIM11A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006717502.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000475910 GCTCCATTGATGAATATAAACAAC pLX_317 14.6% 90.2% 89.6% V5 (many diffs) n/a
2 ccsbBroadEn_05924 pDONR223 100% 90.1% 89.5% None (many diffs) n/a
3 ccsbBroad304_05924 pLX_304 0% 90.1% 89.5% V5 (many diffs) n/a
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