Construct: ORF ccsbBroadEn_06145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016642.1_s300c1, BRDN0000396156
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1AX (1964)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1964 | EIF1AX | eukaryotic translation init... | NM_001412.4 | 99.7% | 99.3% | 10A>G |
| 2 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_004681.4 | 91.8% | 98.6% | (many diffs) |
| 3 | human | 9086 | EIF1AY | eukaryotic translation init... | NM_001278612.1 | 81.4% | 86.8% | (many diffs) |
| 4 | mouse | 66235 | Eif1ax | eukaryotic translation init... | NM_025437.5 | 88.6% | 99.3% | (many diffs) |
| 5 | mouse | 13664 | Eif1a | eukaryotic translation init... | NM_010120.5 | 87.5% | 97.9% | (many diffs) |
| 6 | mouse | 100861898 | Gm21936 | predicted gene, 21936 | XM_006516436.2 | 86.1% | 90.9% | (many diffs) |
| 7 | mouse | 666806 | Gm8300 | predicted gene 8300 | NM_001177565.1 | 85.8% | 93% | (many diffs) |
| 8 | mouse | 100039052 | Gm2022 | predicted pseudogene 2022 | NM_001177574.1 | 85.6% | 92.3% | (many diffs) |
| 9 | mouse | 100861908 | Gm21319 | predicted gene, 21319 | NM_001270722.1 | 85.6% | 92.3% | (many diffs) |
| 10 | mouse | 100039042 | Gm2016 | predicted gene 2016 | NM_001122662.1 | 85.4% | 91.6% | (many diffs) |
| 11 | mouse | 435337 | Gm5662 | predicted gene 5662 | NM_001013824.3 | 85.1% | 91.6% | (many diffs) |
| 12 | mouse | 100042776 | Gm4027 | predicted gene 4027 | NM_001177564.1 | 84.9% | 89.5% | (many diffs) |
| 13 | mouse | 544881 | BB287469 | expressed sequence BB287469 | NM_001177573.1 | 84.9% | 89.5% | (many diffs) |
| 14 | mouse | 100042776 | Gm4027 | predicted gene 4027 | XM_011243969.1 | 84.9% | 89.5% | (many diffs) |
| 15 | mouse | 100503047 | LOC100503047 | eukaryotic translation init... | XM_003085585.1 | 83.5% | 86.2% | (many diffs) |
| 16 | mouse | 100039100 | Gm2046 | predicted gene 2046 | XM_017315327.1 | 74.3% | 78.4% | (many diffs) |
| 17 | mouse | 13664 | Eif1a | eukaryotic translation init... | XM_006525626.3 | 49.6% | 55.5% | (many diffs) |
| 18 | mouse | 266459 | Gm5039 | predicted gene 5039; eukary... | NR_003647.2 | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 498
- ORF length:
- 432
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC CAAGGATAAA GGTAAAGGAG GTAAAAACAG ACGCAGGGGT AAGAATGAGA 121 ATGAATCTGA AAAAAGAGAA CTGGTATTCA AAGAGGATGG TCAGGAGTAT GCTCAGGTAA 181 TCAAAATGTT GGGAAATGGA CGGCTAGAAG CAATGTGTTT CGATGGTGTA AAGAGGTTAT 241 GTCACATCAG AGGAAAATTG AGAAAAAAGG TTTGGATAAA TACCTCGGAC ATTATTTTGG 301 TTGGTCTCCG AGACTACCAG GATAACAAAG CTGATGTAAT TTTAAAATAC AATGCAGACG 361 AAGCTAGAAG TCTGAAGGCA TACGGCGAGC TTCCAGAGCA TGCTAAAATC AATGAAACTG 421 ATACATTTGG TCCTGGAGAT GATGATGAAA TTCAGTTTGA TGACATTGGA GATGATGATG 481 AAGATATTGA TGACATCTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt 541 gcttatcaat ttgttgcaac gaac