Construct: ORF ccsbBroadEn_06145

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016642.1_s300c1, BRDN0000396156
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF1AX (1964)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1964 EIF1AX eukaryotic translation init... NM_001412.4 99.7% 99.3% 10A>G
2 human 9086 EIF1AY eukaryotic translation init... NM_004681.4 91.8% 98.6% (many diffs)
3 human 9086 EIF1AY eukaryotic translation init... NM_001278612.1 81.4% 86.8% (many diffs)
4 mouse 66235 Eif1ax eukaryotic translation init... NM_025437.5 88.6% 99.3% (many diffs)
5 mouse 13664 Eif1a eukaryotic translation init... NM_010120.5 87.5% 97.9% (many diffs)
6 mouse 100861898 Gm21936 predicted gene, 21936 XM_006516436.2 86.1% 90.9% (many diffs)
7 mouse 666806 Gm8300 predicted gene 8300 NM_001177565.1 85.8% 93% (many diffs)
8 mouse 100039052 Gm2022 predicted pseudogene 2022 NM_001177574.1 85.6% 92.3% (many diffs)
9 mouse 100861908 Gm21319 predicted gene, 21319 NM_001270722.1 85.6% 92.3% (many diffs)
10 mouse 100039042 Gm2016 predicted gene 2016 NM_001122662.1 85.4% 91.6% (many diffs)
11 mouse 435337 Gm5662 predicted gene 5662 NM_001013824.3 85.1% 91.6% (many diffs)
12 mouse 100042776 Gm4027 predicted gene 4027 NM_001177564.1 84.9% 89.5% (many diffs)
13 mouse 544881 BB287469 expressed sequence BB287469 NM_001177573.1 84.9% 89.5% (many diffs)
14 mouse 100042776 Gm4027 predicted gene 4027 XM_011243969.1 84.9% 89.5% (many diffs)
15 mouse 100503047 LOC100503047 eukaryotic translation init... XM_003085585.1 83.5% 86.2% (many diffs)
16 mouse 100039100 Gm2046 predicted gene 2046 XM_017315327.1 74.3% 78.4% (many diffs)
17 mouse 13664 Eif1a eukaryotic translation init... XM_006525626.3 49.6% 55.5% (many diffs)
18 mouse 266459 Gm5039 predicted gene 5039; eukary... NR_003647.2 22.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
498
ORF length:
432
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC CAAGGATAAA GGTAAAGGAG GTAAAAACAG ACGCAGGGGT AAGAATGAGA
121ATGAATCTGA AAAAAGAGAA CTGGTATTCA AAGAGGATGG TCAGGAGTAT GCTCAGGTAA
181TCAAAATGTT GGGAAATGGA CGGCTAGAAG CAATGTGTTT CGATGGTGTA AAGAGGTTAT
241GTCACATCAG AGGAAAATTG AGAAAAAAGG TTTGGATAAA TACCTCGGAC ATTATTTTGG
301TTGGTCTCCG AGACTACCAG GATAACAAAG CTGATGTAAT TTTAAAATAC AATGCAGACG
361AAGCTAGAAG TCTGAAGGCA TACGGCGAGC TTCCAGAGCA TGCTAAAATC AATGAAACTG
421ATACATTTGG TCCTGGAGAT GATGATGAAA TTCAGTTTGA TGACATTGGA GATGATGATG
481AAGATATTGA TGACATCTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
541gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)

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