Construct: ORF ccsbBroadEn_06185

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006636.1_s300c1, BRDN0000394783
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EYA1 (2138)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2138 EYA1 EYA transcriptional coactiv... NM_000503.6 99.9% 100% 813A>G
2 human 2138 EYA1 EYA transcriptional coactiv... NM_001370334.1 99.9% 100% 813A>G
3 human 2138 EYA1 EYA transcriptional coactiv... NM_001370335.1 99.9% 100% 813A>G
4 human 2138 EYA1 EYA transcriptional coactiv... NM_172058.4 99.9% 100% 813A>G
5 human 2138 EYA1 EYA transcriptional coactiv... XM_011517483.2 99.7% 99.6% 272_273insCAG;810A>G
6 human 2138 EYA1 EYA transcriptional coactiv... XM_017013208.2 99.7% 99.6% 272_273insCAG;810A>G
7 human 2138 EYA1 EYA transcriptional coactiv... NM_001288574.2 98.9% 98.8% 272_273insCAG;414_415insGGTGCATTGTGGGCA;795A>G
8 human 2138 EYA1 EYA transcriptional coactiv... XM_017013211.2 95.2% 91.6% (many diffs)
9 human 2138 EYA1 EYA transcriptional coactiv... NM_001370333.1 95.2% 95.3% 1_87del;900A>G
10 human 2138 EYA1 EYA transcriptional coactiv... XM_017013202.1 95.2% 95.3% 1_87del;900A>G
11 human 2138 EYA1 EYA transcriptional coactiv... XM_017013203.2 95.1% 95% 1_87del;359_360insCAG;897A>G
12 human 2138 EYA1 EYA transcriptional coactiv... XM_011517484.3 94.4% 94.5% 1_87del;504_505insGGTGCATTGTGGGCA;885A>G
13 human 2138 EYA1 EYA transcriptional coactiv... XM_017013204.2 94.3% 94.2% (many diffs)
14 human 2138 EYA1 EYA transcriptional coactiv... XM_017013212.2 93.8% 93.2% (many diffs)
15 human 2138 EYA1 EYA transcriptional coactiv... XM_017013205.2 91.8% 91.4% (many diffs)
16 human 2138 EYA1 EYA transcriptional coactiv... XM_017013207.2 90.4% 90.4% 1_87del;900A>G;1137_1138ins90
17 human 2138 EYA1 EYA transcriptional coactiv... NM_172059.5 89.6% 89.6% (many diffs)
18 human 2138 EYA1 EYA transcriptional coactiv... NM_001370336.1 89.4% 89.3% (many diffs)
19 human 2138 EYA1 EYA transcriptional coactiv... XM_017013213.1 80.1% 80.2% 0_1ins351;462A>G
20 human 2138 EYA1 EYA transcriptional coactiv... NM_001288575.2 79.3% 79.3% 0_1ins351;66_67insGGTGCATTGTGGGCA;447A>G
21 mouse 14048 Eya1 EYA transcriptional coactiv... XM_011238344.2 90.5% 96.4% (many diffs)
22 mouse 14048 Eya1 EYA transcriptional coactiv... NM_001310459.1 90.4% 96.1% (many diffs)
23 mouse 14048 Eya1 EYA transcriptional coactiv... XM_017315348.1 90.4% 96.1% (many diffs)
24 mouse 14048 Eya1 EYA transcriptional coactiv... NM_010164.2 89.7% 95.6% (many diffs)
25 mouse 14048 Eya1 EYA transcriptional coactiv... XM_006495451.3 89.5% 95.2% (many diffs)
26 mouse 14048 Eya1 EYA transcriptional coactiv... XM_006495446.3 88% 88.3% (many diffs)
27 mouse 14048 Eya1 EYA transcriptional coactiv... NM_001252192.1 84.7% 89.8% (many diffs)
28 mouse 14048 Eya1 EYA transcriptional coactiv... XM_006495452.3 82.5% 82.3% (many diffs)
29 mouse 14048 Eya1 EYA transcriptional coactiv... XM_011238346.2 82.5% 82.3% (many diffs)
30 mouse 14048 Eya1 EYA transcriptional coactiv... XM_017315381.1 82.5% 82.3% (many diffs)
31 mouse 14048 Eya1 EYA transcriptional coactiv... XM_011238347.2 75.5% 79.7% (many diffs)
32 mouse 14048 Eya1 EYA transcriptional coactiv... XM_017315414.1 72.2% 78.5% (many diffs)
33 mouse 14048 Eya1 EYA transcriptional coactiv... XM_017315422.1 72.2% 78.5% (many diffs)
34 mouse 14048 Eya1 EYA transcriptional coactiv... XR_001780756.1 57.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1845
ORF length:
1776
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGAAATGCAG GATCTAACCA GCCCGCATAG CCGTCTGAGT GGTAGTAGTG
121AATCCCCCAG TGGCCCCAAA CTCGGTAACT CTCATATAAA TAGTAATTCC ATGACTCCCA
181ATGGCACCGA AGTTAAAACA GAGCCAATGA GCAGCAGTGA AACAGCTTCA ACGACAGCCG
241ACGGGTCTTT AAACAATTTC TCAGGTTCAG CAATTGGGAG CAGTAGTTTC AGCCCACGAC
301CAACTCACCA GTTCTCTCCA CCACAGATTT ACCCTTCCAA CAGACCATAC CCACATATTC
361TCCCTACCCC TTCCTCACAA ACTATGGCTG CATATGGGCA AACACAGTTT ACCACAGGAA
421TGCAACAAGC TACAGCCTAT GCCACGTACC CACAGCCAGG ACAGCCGTAC GGCATTTCCT
481CATATGGTGC ATTGTGGGCA GGCATCAAGA CTGAAGGTGG ATTGTCACAG TCTCAGTCAC
541CTGGACAGAC AGGATTTCTC AGCTATGGCA CAAGCTTCAG TACCCCTCAA CCTGGACAGG
601CACCATACAG CTACCAGATG CAAGGTAGCA GTTTTACAAC ATCATCAGGA ATATATACAG
661GAAATAATTC ACTCACAAAT TCCTCTGGAT TTAATAGTTC ACAGCAGGAC TATCCGTCTT
721ATCCCAGTTT TGGCCAGGGT CAGTACGCAC AGTATTATAA CAGCTCACCG TATCCAGCAC
781ATTATATGAC CAGCAGCAAC ACCAGCCCAA CGACACCATC CACCAATGCC ACTTACCAGC
841TTCAAGAACC GCCATCTGGC ATCACCAGCC AAGCAGTTAC GGATCCCACA GCAGAGTACA
901GCACAATCCA CAGCCCATCA ACACCCATTA AAGATTCAGA TTCTGATCGA TTGCGTCGAG
961GTTCAGATGG GAAATCACGT GGACGGGGCC GAAGAAACAA TAATCCTTCA CCTCCCCCAG
1021ATTCTGATCT TGAGAGAGTG TTCATCTGGG ACTTGGATGA GACAATCATT GTTTTCCACT
1081CCTTGCTTAC TGGGTCCTAC GCCAACAGAT ATGGGAGGGA TCCACCCACT TCAGTTTCCC
1141TTGGACTGCG AATGGAAGAA ATGATTTTCA ACTTGGCAGA CACACATTTA TTTTTTAATG
1201ACTTAGAAGA ATGTGACCAA GTCCATATAG ATGATGTTTC TTCAGATGAT AACGGACAGG
1261ACCTAAGCAC ATATAACTTT GGAACAGATG GCTTTCCTGC TGCAGCAACC AGTGCTAACT
1321TATGTTTGGC AACTGGTGTA CGGGGCGGTG TGGACTGGAT GAGAAAGTTG GCCTTCCGCT
1381ACAGACGGGT AAAAGAGATC TACAACACCT ACAAAAATAA TGTTGGAGGT CTGCTTGGTC
1441CAGCTAAGAG GGAAGCCTGG CTGCAGTTGA GGGCCGAAAT TGAAGCCCTG ACCGACTCCT
1501GGTTGACACT GGCCCTGAAA GCACTCTCGC TCATTCACTC CCGGACAAAC TGTGTGAATA
1561TTTTAGTAAC AACTACTCAG CTCATCCCAG CATTGGCGAA AGTCCTGCTG TATGGGTTAG
1621GAATTGTATT TCCAATAGAA AATATTTACA GTGCAACTAA AATAGGAAAA GAAAGCTGTT
1681TTGAGAGAAT AATTCAAAGG TTTGGAAGAA AAGTGGTGTA TGTTGTTATA GGAGATGGTG
1741TAGAAGAAGA ACAAGGAGCA AAAAAGCACG CGATGCCCTT CTGGAGGATC TCCAGCCACT
1801CGGACCTCAT GGCCCTGCAC CATGCCTTGG AACTGGAGTA CCTGTTGCCA ACTTTCTTGT
1861ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)