Construct: ORF ccsbBroadEn_06194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003740.1_s300c1, BRDN0000396063
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSL4 (2182)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | NM_001318510.2 | 99.9% | 100% | 1641A>G |
| 2 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | NM_004458.3 | 99.9% | 100% | 1641A>G |
| 3 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | XM_006724635.2 | 99.9% | 100% | 1641A>G |
| 4 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | NM_001318509.2 | 94.1% | 94.2% | 1_123del;1764A>G |
| 5 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | NM_022977.2 | 94.1% | 94.2% | 1_123del;1764A>G |
| 6 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | XM_005262109.2 | 94.1% | 94.2% | 1_123del;1764A>G |
| 7 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | XM_011530888.2 | 94.1% | 94.2% | 1_123del;1764A>G |
| 8 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | XM_011530889.2 | 94.1% | 94.2% | 1_123del;1764A>G |
| 9 | human | 2182 | ACSL4 | acyl-CoA synthetase long ch... | XM_024452351.1 | 93.3% | 93.4% | 1_141del;1782A>G |
| 10 | mouse | 50790 | Acsl4 | acyl-CoA synthetase long-ch... | NM_001033600.1 | 92.2% | 96.7% | (many diffs) |
| 11 | mouse | 50790 | Acsl4 | acyl-CoA synthetase long-ch... | NM_019477.3 | 92.2% | 96.7% | (many diffs) |
| 12 | mouse | 50790 | Acsl4 | acyl-CoA synthetase long-ch... | XM_011247842.2 | 92.2% | 96.7% | (many diffs) |
| 13 | mouse | 50790 | Acsl4 | acyl-CoA synthetase long-ch... | XM_011247843.2 | 92.2% | 96.7% | (many diffs) |
| 14 | mouse | 50790 | Acsl4 | acyl-CoA synthetase long-ch... | NM_207625.2 | 86.9% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2076
- ORF length:
- 2010
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AAAGAGAATA AAAGCTAAGC CCACTTCAGA CAAACCTGGA AGTCCATATC 121 GCTCTGTCAC ACACTTCGAC TCACTAGCTG TAATAGACAT CCCTGGAGCA GATACTCTGG 181 ATAAATTATT TGACCATGCT GTATCCAAGT TTGGGAAGAA GGACAGCCTT GGGACCAGGG 241 AAATCCTAAG TGAAGAAAAT GAAATGCAGC CAAATGGAAA AGTTTTTAAG AAGTTAATTC 301 TTGGGAATTA TAAATGGATG AACTATCTTG AAGTGAATCG CAGAGTGAAT AACTTTGGTA 361 GTGGACTCAC TGCACTGGGA CTAAAACCAA AGAACACCAT TGCCATCTTC TGTGAGACCA 421 GGGCCGAATG GATGATTGCA GCACAGACCT GCTTTAAGTA CAACTTTCCT CTTGTGACTT 481 TATATGCCAC ACTTGGCAAA GAAGCAGTAG TTCATGGGCT AAATGAATCT GAGGCTTCCT 541 ATCTGATTAC CAGTGTTGAA CTTCTGGAAA GTAAACTTAA GACTGCATTG TTAGATATCA 601 GTTGTGTTAA ACATATCATT TATGTGGACA ATAAGGCTAT CAATAAAGCA GAGTACCCTG 661 AAGGATTTGA GATTCACAGC ATGCAATCAG TAGAAGAGTT GGGATCTAAC CCAGAAAACT 721 TGGGCATTCC TCCAAGTAGA CCAACGCCTT CAGACATGGC CATTGTTATG TATACTAGTG 781 GTTCTACTGG CCGACCTAAG GGAGTGATGA TGCATCATAG CAATTTGATA GCTGGAATGA 841 CAGGCCAGTG TGAAAGAATA CCTGGACTGG GACCGAAGGA CACATATATT GGCTACTTGC 901 CTTTGGCTCA TGTGCTAGAA CTGACAGCAG AGATATCTTG CTTTACCTAT GGCTGCAGGA 961 TTGGATATTC TTCTCCGCTT ACACTCTCTG ACCAGTCCAG CAAAATTAAA AAAGGAAGCA 1021 AAGGAGACTG TACTGTACTG AAGCCCACAC TTATGGCTGC TGTTCCGGAA ATCATGGATA 1081 GAATTTATAA GAATGTTATG AGCAAAGTCC AAGAGATGAA TTATATTCAG AAAACTCTGT 1141 TCAAGATAGG GTATGATTAC AAATTGGAAC AGATCAAAAA GGGATATGAT GCACCTCTTT 1201 GCAATCTGTT ACTGTTTAAA AAGGTCAAGG CCCTGCTGGG AGGGAATGTC CGCATGATGC 1261 TGTCTGGAGG GGCCCCGCTA TCTCCTCAGA CACACCGATT CATGAATGTC TGCTTCTGCT 1321 GCCCAATTGG CCAGGGTTAT GGACTGACAG AATCATGTGG TGCTGGGACA GTTACTGAAG 1381 TAACTGACTA TACTACTGGC AGAGTTGGAG CACCTCTTAT TTGCTGTGAA ATTAAGCTAA 1441 AAGACTGGCA AGAAGGCGGT TATACAATTA ATGACAAGCC AAACCCCAGA GGTGAAATCG 1501 TAATTGGTGG ACAGAACATC TCCATGGGAT ATTTTAAAAA TGAAGAGAAA ACAGCAGAAG 1561 ATTATTCTGT GGATGAAAAT GGACAAAGGT GGTTTTGCAC TGGTGATATT GGAGAATTCC 1621 ATCCCGATGG ATGTTTACAG ATTATAGATC GTAAGAAAGA TCTAGTGAAG TTACAAGCAG 1681 GAGAGTATGT ATCTCTTGGG AAAGTGGAAG CTGCACTGAA GAATTGTCCA CTTATTGACA 1741 ACATCTGTGC TTTTGCCAAA AGTGATCAGT CCTATGTGAT CAGTTTTGTG GTTCCTAACC 1801 AGAAAAGGTT GACACTTTTG GCACAACAGA AAGGGGTAGA AGGAACTTGG GTTGATATCT 1861 GCAATAATCC TGCTATGGAA GCTGAAATAC TGAAAGAAAT TCGAGAAGCT GCAAATGCCA 1921 TGAAATTGGA GCGATTTGAA ATTCCAATCA AGGTTCGATT AAGCCCAGAG CCATGGACCC 1981 CTGAAACTGG TTTGGTAACT GATGCTTTCA AACTGAAAAG GAAGGAGCTG AGGAACCATT 2041 ACCTCAAAGA CATTGAACGA ATGTATGGGG GCAAATACCC AACTTTCTTg tacaaagttg 2101 gcattataag aaagcattgc ttatcaattt gttgcaacga ac