Construct: ORF ccsbBroadEn_06338

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011781.1_s300c1, BRDN0000391825
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GTF2A2 (2958)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2958 GTF2A2 general transcription facto... NM_001320929.2 99.6% 100% 315C>T
2 human 2958 GTF2A2 general transcription facto... NM_001320930.2 99.6% 100% 315C>T
3 human 2958 GTF2A2 general transcription facto... NM_004492.3 99.6% 100% 315C>T
4 mouse 235459 Gtf2a2 general transcription facto... NM_001039519.2 95.1% 99% (many diffs)
5 mouse 235459 Gtf2a2 general transcription facto... NM_001282083.1 95.1% 99% (many diffs)
6 mouse 235459 Gtf2a2 general transcription facto... XM_006511116.2 95.1% 99% (many diffs)
7 mouse 235459 Gtf2a2 general transcription facto... NM_001282084.1 55.7% 55% (many diffs)
8 mouse 235459 Gtf2a2 general transcription facto... NR_104093.1 30% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
393
ORF length:
327
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC ATATCAGTTA TACAGAAATA CTACTTTGGG AAACAGTCTT CAGGAGAGCC
121TAGATGAGCT CATACAGTCT CAACAGATCA CCCCCCAACT TGCCCTTCAA GTTCTACTTC
181AGTTTGATAA GGCTATAAAT GCAGCACTGG CTCAGAGGGT CAGGAACAGA GTCAATTTCA
241GGGGCTCTCT AAATACGTAC AGATTCTGCG ATAATGTGTG GACTTTTGTA CTGAATGATG
301TTGAATTCAG AGAGGTGACA GAACTTATTA AAGTGGATAA AGTGAAAATT GTAGCCTGTG
361ATGGTAAAAA TACTGGCTCT AATACTACAG AATGCCCAAC TTTCTTGTAC AAAGTtggca
421ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)