Transcript: Mouse NR_104093.1

Mus musculus general transcription factor II A, 2 (Gtf2a2), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Gtf2a2 (235459)
Length:
1034
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_104093.1
NBCI Gene record:
Gtf2a2 (235459)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_104093.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000220191 GCTCATACAGTCTCAACAGAT pLKO.1 224 3UTR 100% 4.950 3.960 N GTF2A2 n/a
2 TRCN0000280343 GCTCATACAGTCTCAACAGAT pLKO_005 224 3UTR 100% 4.950 3.960 N GTF2A2 n/a
3 TRCN0000431953 CATACAGATTCTGCGATAATG pLKO_005 491 3UTR 100% 13.200 9.240 N Gtf2a2 n/a
4 TRCN0000431481 GTCAGGAACAGAGTCAATTTC pLKO_005 315 3UTR 100% 13.200 9.240 N Gtf2a2 n/a
5 TRCN0000434904 GTGGTTGCCTTCATAAGATTA pLKO_005 859 3UTR 100% 13.200 9.240 N Gtf2a2 n/a
6 TRCN0000417148 ATGCGACTCTGTTCTCGTAAT pLKO_005 783 3UTR 100% 10.800 7.560 N Gtf2a2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_104093.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06338 pDONR223 100% 30% None (many diffs) n/a
2 ccsbBroad304_06338 pLX_304 0% 30% V5 (many diffs) n/a
3 TRCN0000479122 GATCCGATCTACCTAATTGTCTCC pLX_317 100% 30% V5 (many diffs) n/a
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