Transcript: Human NM_001320929.2

Homo sapiens general transcription factor IIA subunit 2 (GTF2A2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
GTF2A2 (2958)
Length:
1631
CDS:
232..561

Additional Resources:

NCBI RefSeq record:
NM_001320929.2
NBCI Gene record:
GTF2A2 (2958)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001320929.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000220190 GCTCTCTAAATACGTACAGAT pLKO.1 410 CDS 100% 4.950 6.930 N GTF2A2 n/a
2 TRCN0000280278 GCTCTCTAAATACGTACAGAT pLKO_005 410 CDS 100% 4.950 6.930 N GTF2A2 n/a
3 TRCN0000431481 GTCAGGAACAGAGTCAATTTC pLKO_005 385 CDS 100% 13.200 9.240 N Gtf2a2 n/a
4 TRCN0000220192 CTTCAGTTTGATAAGGCTATA pLKO.1 343 CDS 100% 10.800 7.560 N GTF2A2 n/a
5 TRCN0000297812 CTTCAGTTTGATAAGGCTATA pLKO_005 343 CDS 100% 10.800 7.560 N GTF2A2 n/a
6 TRCN0000220193 CTGGCTCCAATACTACAGAAT pLKO.1 539 CDS 100% 4.950 3.465 N GTF2A2 n/a
7 TRCN0000280279 CTGGCTCCAATACTACAGAAT pLKO_005 539 CDS 100% 4.950 3.465 N GTF2A2 n/a
8 TRCN0000220191 GCTCATACAGTCTCAACAGAT pLKO.1 294 CDS 100% 4.950 3.465 N GTF2A2 n/a
9 TRCN0000280343 GCTCATACAGTCTCAACAGAT pLKO_005 294 CDS 100% 4.950 3.465 N GTF2A2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001320929.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06338 pDONR223 100% 99.6% 100% None 315C>T n/a
2 ccsbBroad304_06338 pLX_304 0% 99.6% 100% V5 315C>T n/a
3 TRCN0000479122 GATCCGATCTACCTAATTGTCTCC pLX_317 100% 99.6% 100% V5 315C>T n/a
Download CSV