Construct: ORF ccsbBroadEn_06419

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017790.1_s300c1, BRDN0000398639
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HTR5A (3361)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3361 HTR5A 5-hydroxytryptamine recepto... NM_024012.3 99.8% 99.7% 12T>A;43C>T
2 mouse 15563 Htr5a 5-hydroxytryptamine (seroto... NM_008314.2 84.2% 87.6% (many diffs)
3 mouse 15563 Htr5a 5-hydroxytryptamine (seroto... XM_006535631.2 78.2% 77.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1140
ORF length:
1071
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGATTTACCA GTGAACCTAA CCTCCTTTTC CCTCTCCACC TCCTCCCCTT
121TGGAGACCAA CCACAGCCTC GGCAAAGACG ACCTGCGCCC CAGCTCGCCC CTGCTCTCGG
181TCTTCGGAGT GCTTATTCTC ACCTTGCTGG GCTTTCTGGT GGCGGCGACG TTCGCCTGGA
241ACCTGCTGGT GCTGGCGACC ATCCTCCGTG TACGCACCTT CCACCGCGTG CCCCACAACC
301TGGTGGCATC CATGGCCGTC TCGGATGTCC TGGTGGCCGC GCTGGTCATG CCGCTGAGCC
361TGGTGCACGA GCTGTCCGGG CGCCGCTGGC AGCTAGGTCG GAGGCTGTGC CAGCTTTGGA
421TCGCGTGCGA CGTGCTTTGC TGCACGGCCA GCATCTGGAA CGTGACGGCC ATAGCCCTGG
481ACCGCTACTG GTCCATCACG CGCCACATGG AATACACGCT CCGCACCCGC AAGTGCGTCT
541CCAACGTCAT GATCGCGCTC ACCTGGGCAC TCTCCGCTGT CATCTCTCTG GCCCCGCTGC
601TTTTTGGCTG GGGAGAGACG TACTCTGAGG GCAGCGAGGA GTGCCAGGTA AGCCGCGAGC
661CTTCCTACGC CGTGTTCTCC ACCGTAGGCG CCTTCTACCT GCCGCTCTGT GTGGTGCTCT
721TCGTGTACTG GAAGATCTAC AAGGCTGCCA AGTTCCGCGT GGGCTCCAGG AAGACCAATA
781GCGTCTCACC CATATCCGAA GCTGTGGAGG TGAAGGACTC TGCCAAACAG CCCCAGATGG
841TGTTCACGGT CCGCCACGCC ACCGTCACCT TCCAGCCAGA AGGGGACACG TGGCGGGAGC
901AGAAGGAGCA GCGGGCCGCC CTCATGGTGG GCATCCTCAT TGGCGTGTTC GTGCTCTGCT
961GGATCCCCTT CTTTCTCACC GAGCTCATCA GTCCCCTCTG CTCCTGTGAC ATCCCCGCCA
1021TCTGGAAAAG CATCTTCCTG TGGCTTGGCT ACTCCAACTC CTTCTTTAAC CCCCTGATCT
1081ATACGGCTTT CAACAAGAAC TACAACAGCG CCTTCAAGAA CTTCTTTTCT AGGCAACACT
1141TGCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1201acgaac

Download FASTA (ORF) (Full)