Construct: ORF ccsbBroadEn_06584

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009354.1_s300c1, BRDN0000457096
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MKLN1 (4289)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4289 MKLN1 muskelin 1 NM_013255.5 99.9% 100% 1324C>A;1575T>C
2 human 4289 MKLN1 muskelin 1 NM_001145354.2 95.8% 94.7% (many diffs)
3 human 4289 MKLN1 muskelin 1 XM_006715993.3 94.5% 94.5% 1324C>A;1575T>C;2087_2088delCAins119
4 human 4289 MKLN1 muskelin 1 XM_024446766.1 87.3% 87.4% 0_1ins276;1048C>A;1299T>C
5 human 4289 MKLN1 muskelin 1 XM_024446767.1 87.3% 87.4% 0_1ins276;1048C>A;1299T>C
6 human 4289 MKLN1 muskelin 1 NM_001321316.2 71.7% 71.8% 0_1ins621;703C>A;954T>C
7 human 4289 MKLN1 muskelin 1 XM_011516224.3 63.1% 63.2% 1324C>A;1396_1399delTTCC;1401_1402ins808
8 mouse 27418 Mkln1 muskelin 1, intracellular m... NM_013791.2 92.9% 99.1% (many diffs)
9 mouse 27418 Mkln1 muskelin 1, intracellular m... XM_017321605.1 66.6% 71.1% (many diffs)
10 mouse 27418 Mkln1 muskelin 1, intracellular m... XM_017321606.1 51.2% 54.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2274
ORF length:
2205
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAGAAAG
61TTGGCACCAT GGCGGCTGGC GGAGCTGTCG CTGCGGCGCC CGAGTGCCGG CTTCTCCCCT
121ACGCGCTACA CAAGTGGAGC TCCTTTTCCT CCACCTACCT TCCCGAGAAC ATTTTAGTGG
181ACAAACCAAA TGACCAATCT TCAAGATGGT CTTCAGAGAG CAACTATCCT CCCCAGTACT
241TGATTCTAAA GCTCGAAAGG CCTGCTATAG TTCAGAATAT CACATTTGGA AAATATGAGA
301AAACTCATGT TTGCAATTTG AAGAAATTTA AAGTCTTTGG TGGAATGAAT GAAGAAAATA
361TGACAGAGCT GTTGTCCAGT GGCTTAAAGA ATGATTATAA CAAAGAAACA TTCACCTTGA
421AGCATAAAAT TGATGAACAG ATGTTCCCTT GTCGATTCAT TAAAATAGTT CCACTCTTGT
481CCTGGGGACC CAGCTTTAAC TTTAGCATCT GGTATGTTGA ACTTAGTGGC ATTGATGATC
541CTGATATAGT ACAACCTTGT CTCAACTGGT ATAGCAAGTA CCGTGAACAG GAAGCTATTC
601GCCTTTGCCT AAAACACTTC AGACAACACA ACTATACAGA AGCTTTTGAG TCACTGCAAA
661AGAAAACCAA GATTGCACTG GAACATCCCA TGTTAACAGA TATTCATGAC AAGCTGGTGT
721TGAAGGGTGA TTTTGATGCT TGCGAAGAGT TGATTGAAAA GGCTGTAAAT GATGGCTTGT
781TCAATCAGTA TATCAGTCAA CAGGAATATA AGCCACGATG GAGTCAAATC ATTCCCAAAA
841GTACCAAAGG TGATGGGGAA GATAACCGTC CAGGAATGAG AGGAGGCCAT CAGATGGTTA
901TTGATGTTCA AACAGAGACT GTTTATTTGT TTGGTGGCTG GGATGGAACA CAAGATCTTG
961CTGACTTCTG GGCGTACAGT GTGAAGGAGA ACCAGTGGAC ATGTATCTCT AGAGACACTG
1021AAAAAGAGAA TGGTCCTAGT GCCAGATCGT GTCATAAAAT GTGCATTGAT ATTCAACGGA
1081GGCAAATCTA CACATTGGGG CGTTACTTGG ATTCCTCTGT GAGGAACAGC AAATCTCTGA
1141AAAGTGACTT CTATCGTTAT GACATTGATA CAAACACATG GATGTTACTA AGTGAGGATA
1201CTGCTGCTGA TGGAGGGCCG AAATTGGTGT TTGATCATCA GATGTGTATG GACTCAGAAA
1261AACATATGAT CTACACTTTT GGTGGTAGAA TTTTGACTTG TAATGGCAGC GTAGATGACA
1321GCAGAGCCAG TGAACCACAA TTCAGTGGCT TGTTTGCTTT CAACTGTCAA TGTCAAACCT
1381GGAAACTTCT TAGAGAGGAC TCCTGTAATG CTGGGCCTGA GGACATCCAG TCTCGAATAG
1441GACACTGCAT GTTATTCCAC TCAAAAAATC GTTGCTTATA TGTATTTGGT GGCCAGCGAT
1501CAAAGACCTA TTTGAATGAT TTCTTTAGTT ATGATGTGGA CTCTGATCAT GTAGACATAA
1561TATCAGATGG CACCAAGAAA GACTCTGGGA TGGTTCCAAT GACAGGATTT ACACAGAGAG
1621CAACTATTGA TCCAGAACTG AACGAAATAC ACGTCTTATC TGGACTCAGC AAAGATAAGG
1681AAAAGAGGGA AGAAAATGTT AGAAATTCAT TCTGGATTTA TGACATTGTG AGGAATAGTT
1741GGTCTTGTGT CTATAAGAAT GATCAAGCTG CAAAGGATAA TCCAACTAAA AGTCTTCAGG
1801AAGAAGAACC ATGTCCAAGG TTTGCCCATC AGCTTGTATA CGATGAGCTA CACAAGGTTC
1861ATTACTTATT TGGTGGGAAT CCAGGAAAAT CTTGCTCTCC AAAGATGAGA TTAGATGACT
1921TCTGGTCACT GAAGTTGTGT AGACCTTCAA AAGATTATTT ACTGAGGCAT TGCAAGTACC
1981TCATAAGAAA ACACAGGTTT GAAGAAAAGG CCCAAGTGGA TCCCCTTAGT GCTCTGAAAT
2041ATTTACAAAA TGATCTTTAT ATAACTGTGG ATCATTCAGA CCCAGAAGAG ACAAAAGAGT
2101TTCAGCTCCT GGCATCAGCT CTATTCAAAT CTGGTTCAGA TTTTACAGCT CTGGGCTTTT
2161CTGATGTGGA TCACACCTAT GCTCAAAGAA CTCAGCTCTT TGACACCTTA GTAAATTTCT
2221TTCCTGACAG CATGACTCCT CCTAAAGGCA ACCTGGTAGA CCTCATCACA CTGTTGCCAA
2281CTTTCTTTTA CAAAGTTGGc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)