Construct: ORF ccsbBroadEn_06671

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007681.1_s300c1, BRDN0000457414
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OPA1 (4976)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_015560.2 99.9% 99.8% 473G>A;2109T>C
2 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130834.3 98% 98% 473G>A;555_608del;2163T>C
3 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130832.3 97.2% 96.5% (many diffs)
4 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130836.3 96.2% 96.1% 473G>A;622_732del;2220T>C
5 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130831.3 96.2% 96.2% 447_448ins108;2001T>C
6 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130837.2 94.5% 94.4% (many diffs)
7 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130835.2 93.6% 92.9% (many diffs)
8 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_130833.2 92.6% 92.6% 447_448ins108;514_624del;2112T>C
9 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_001354664.2 87% 86.9% 0_1ins372;101G>A;1737T>C
10 human 4976 OPA1 OPA1 mitochondrial dynamin ... NM_001354663.2 80.2% 80.2% (many diffs)
11 human 4976 OPA1 OPA1 mitochondrial dynamin ... XR_001740159.2 45.3% (many diffs)
12 human 4976 OPA1 OPA1 mitochondrial dynamin ... XR_001740158.2 44.1% (many diffs)
13 mouse 74143 Opa1 OPA1, mitochondrial dynamin... NM_133752.3 88.5% 96.2% (many diffs)
14 mouse 74143 Opa1 OPA1, mitochondrial dynamin... NM_001199177.1 87% 94.4% (many diffs)
15 mouse 74143 Opa1 OPA1, mitochondrial dynamin... XM_006522657.3 78.6% 85.3% (many diffs)
16 mouse 74143 Opa1 OPA1, mitochondrial dynamin... XM_030249307.1 78.6% 85.3% (many diffs)
17 mouse 74143 Opa1 OPA1, mitochondrial dynamin... XM_006522654.4 77.4% 84% (many diffs)
18 mouse 74143 Opa1 OPA1, mitochondrial dynamin... XM_006522655.3 77.4% 84% (many diffs)
19 mouse 74143 Opa1 OPA1, mitochondrial dynamin... XM_006522656.4 77.4% 84% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2949
ORF length:
2880
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GTGGCGACTA CGTCGGGCCG CTGTGGCCTG TGAGGTCTGC CAGTCTTTAG
121TGAAACACAG CTCTGGAATA AAAGGAAGTT TACCACTACA AAAACTACAT CTGGTTTCAC
181GAAGCATTTA TCATTCACAT CATCCTACCT TAAAGCTTCA ACGACCCCAA TTAAGGACAT
241CCTTTCAGCA GTTCTCTTCT CTGACAAACC TTCCTTTACG TAAACTGAAA TTCTCTCCAA
301TTAAATATGG CTACCAGCCT CGCAGGAATT TTTGGCCAGC AAGATTAGCT ACGAGACTCT
361TAAAACTTCG CTATCTCATA CTAGGATCGG CTGTTGGGGG TGGCTACACA GCCAAAAAGA
421CTTTTGATCA GTGGAAAGAT ATGATACCGG ACCTTAGTGA ATATAAATGG ATTGTGCCTG
481ACATTGTGTG GGAAATTGAT GAGTATATCG ATTTTGAGAA AATTAGAAAA GCCCTTCCTA
541ATTCAGAAGA CCTTGTAAAG TTAGCACCAG ACTTTGACAA GATTGTTGAA AGCCTTAGCT
601TATTGAAGGA CTTTTTTACC TCAGGTTCTC CGGAAGAAAC GGCGTTTAGA GCAACAGATC
661GTGGATCTGA AAGTGACAAG CATTTTAGAA AGGTGTCAGA CAAAGAGAAA ATTGACCAAC
721TTCAGGAAGA ACTTCTGCAC ACTCAGTTGA AGTATCAGAG AATCTTGGAA CGATTAGAAA
781AGGAGAACAA AGAATTGAGA AAATTAGTAT TGCAGAAAGA TGACAAAGGC ATTCATCATA
841GAAAGCTTAA GAAATCTTTG ATTGACATGT ATTCTGAAGT TCTTGATGTT CTCTCTGATT
901ATGATGCCAG TTATAATACG CAAGATCATC TGCCACGGGT TGTTGTGGTT GGAGATCAGA
961GTGCTGGAAA GACTAGTGTG TTGGAAATGA TTGCCCAAGC TCGAATATTC CCAAGAGGAT
1021CTGGGGAGAT GATGACACGT TCTCCAGTTA AGGTGACTCT GAGTGAAGGT CCTCACCATG
1081TGGCCCTATT TAAAGATAGT TCTCGGGAGT TTGATCTTAC CAAAGAAGAA GATCTTGCAG
1141CATTAAGACA TGAAATAGAA CTTCGAATGA GGAAAAATGT GAAAGAAGGC TGTACCGTTA
1201GCCCTGAGAC CATATCCTTA AATGTAAAAG GCCCTGGACT ACAGAGGATG GTGCTTGTTG
1261ACTTACCAGG TGTGATTAAT ACTGTGACAT CAGGCATGGC TCCTGACACA AAGGAAACTA
1321TTTTCAGTAT CAGCAAAGCT TACATGCAGA ATCCTAATGC CATCATACTG TGTATTCAAG
1381ATGGATCTGT GGATGCTGAA CGCAGTATTG TTACAGACTT GGTCAGTCAA ATGGACCCTC
1441ATGGAAGGAG AACCATATTC GTTTTGACCA AAGTAGACCT GGCAGAGAAA AATGTAGCCA
1501GTCCAAGCAG GATTCAGCAG ATAATTGAAG GAAAGCTCTT CCCAATGAAA GCTTTAGGTT
1561ATTTTGCTGT TGTAACAGGA AAAGGGAACA GCTCTGAAAG CATTGAAGCT ATAAGAGAAT
1621ATGAAGAAGA GTTTTTTCAG AATTCAAAGC TCCTAAAGAC AAGCATGCTA AAGGCACACC
1681AAGTGACTAC AAGAAATTTA AGCCTTGCAG TATCAGACTG CTTTTGGAAA ATGGTACGAG
1741AGTCTGTTGA ACAACAGGCT GATAGTTTCA AAGCAACACG TTTTAACCTT GAAACTGAAT
1801GGAAGAATAA CTATCCTCGC CTGCGGGAAC TTGACCGGAA TGAACTATTT GAAAAAGCTA
1861AAAATGAAAT CCTTGATGAA GTTATCAGTC TGAGCCAGGT TACACCAAAA CATTGGGAGG
1921AAATCCTTCA ACAATCTTTG TGGGAAAGAG TATCAACTCA TGTGATTGAA AACATCTACC
1981TTCCAGCTGC GCAGACCATG AATTCAGGAA CTTTTAACAC CACAGTGGAT ATCAAGCTTA
2041AACAGTGGAC TGATAAACAA CTTCCTAATA AAGCAGTAGA GGTTGCTTGG GAGACCCTAC
2101AAGAAGAATT TTCCCGCTTT ATGACAGAAC CGAAAGGGAA AGAGCATGAT GACATATTTG
2161ATAAACTTAA AGAGGCCGTT AAGGAAGAAA GTATTAAACG ACACAAGTGG AATGACTTTG
2221CGGAGGACAG CTTGAGGGTT ATTCAACACA ATGCTTTGGA AGACCGATCC ATATCTGATA
2281AACAGCAATG GGATGCAGCT ATTTATTTTA TGGAAGAGGC TCTGCAGGCT CGTCTCAAGG
2341ATACTGAAAA TGCAATTGAA AACATGGTGG GTCCAGACTG GAAAAAGAGG TGGTTATACT
2401GGAAGAATCG GACCCAAGAA CAGTGTGTTC ACAATGAAAC CAAGAATGAA TTGGAGAAGA
2461TGTTGAAATG TAATGAGGAG CACCCAGCTT ATCTTGCAAG TGATGAAATA ACCACAGTCC
2521GGAAGAACCT TGAATCCCGA GGAGTAGAAG TAGATCCAAG CTTGATTAAG GATACTTGGC
2581ATCAAGTTTA TAGAAGACAT TTTTTAAAAA CAGCTCTAAA CCATTGTAAC CTTTGTCGAA
2641GAGGTTTTTA TTACTACCAA AGGCATTTTG TAGATTCTGA GTTGGAATGC AATGATGTGG
2701TCTTGTTTTG GCGTATACAG CGCATGCTTG CTATCACCGC AAATACTTTA AGGCAACAAC
2761TTACAAATAC TGAAGTTAGG CGATTAGAGA AAAATGTTAA AGAGGTATTG GAAGATTTTG
2821CTGAAGATGG TGAGAAGAAG ATTAAATTGC TTACTGGTAA ACGCGTTCAA CTGGCGGAAG
2881ACCTCAAGAA AGTTAGAGAA ATTCAAGAAA AACTTGATGC TTTCATTGAA GCTCTTCATC
2941AGGAGAAATT GCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa
3001tttgttgcaa cgaac

Download FASTA (ORF) (Full)