Transcript: Human NM_001354663.2

Homo sapiens OPA1 mitochondrial dynamin like GTPase (OPA1), transcript variant 9, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
OPA1 (4976)
Length:
6325
CDS:
601..3114

Additional Resources:

NCBI RefSeq record:
NM_001354663.2
NBCI Gene record:
OPA1 (4976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001354663.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000082846 CCGGACCTTAGTGAATATAAA pLKO.1 607 CDS 100% 15.000 21.000 N OPA1 n/a
2 TRCN0000299221 CCGGACCTTAGTGAATATAAA pLKO_005 607 CDS 100% 15.000 21.000 N OPA1 n/a
3 TRCN0000082845 CGGACCTTAGTGAATATAAAT pLKO.1 608 CDS 100% 15.000 21.000 N OPA1 n/a
4 TRCN0000303506 GATTGCCCAAGCTCGAATATT pLKO_005 1152 CDS 100% 15.000 21.000 N OPA1 n/a
5 TRCN0000303566 TGATTATGATGCCAGTTATAA pLKO_005 1059 CDS 100% 15.000 21.000 N OPA1 n/a
6 TRCN0000082847 CCGCTTTATGACAGAACCGAA pLKO.1 2277 CDS 100% 2.640 3.696 N OPA1 n/a
7 TRCN0000082844 CGGGAGTTTGATCTTACCAAA pLKO.1 1267 CDS 100% 4.950 3.960 N OPA1 n/a
8 TRCN0000303567 CTCACCATGTGGCCCTATTTA pLKO_005 1235 CDS 100% 15.000 10.500 N OPA1 n/a
9 TRCN0000082843 GCCCTCTTTGTATTCCCATTT pLKO.1 5482 3UTR 100% 10.800 6.480 N OPA1 n/a
10 TRCN0000299296 GCCCTCTTTGTATTCCCATTT pLKO_005 5482 3UTR 100% 10.800 6.480 N OPA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001354663.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06671 pDONR223 100% 80.2% 80.2% None (many diffs) n/a
2 ccsbBroad304_06671 pLX_304 0% 80.2% 80.2% V5 (many diffs) n/a
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