Construct: ORF ccsbBroadEn_06685

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001176.1_s300c1, BRDN0000394063
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAH (5053)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5053 PAH phenylalanine hydroxylase NM_000277.3 99.8% 99.7% 108A>G;548A>G
2 human 5053 PAH phenylalanine hydroxylase NM_001354304.2 99.8% 99.7% 108A>G;548A>G
3 human 5053 PAH phenylalanine hydroxylase XM_017019370.2 52.4% 51.7% (many diffs)
4 mouse 18478 Pah phenylalanine hydroxylase NM_008777.3 87% 92% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1422
ORF length:
1356
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC CACTGCGGTC CTGGAAAACC CAGGCTTGGG CAGGAAACTC TCTGACTTTG
121GACAGGAAAC AAGCTATATT GAAGACAACT GCAATCAAAA TGGTGCCATA TCGCTGATCT
181TCTCACTCAA AGAAGAAGTT GGTGCATTGG CCAAAGTATT GCGCTTATTT GAGGAGAATG
241ATGTAAACCT GACCCACATT GAATCTAGAC CTTCTCGTTT AAAGAAAGAT GAGTATGAAT
301TTTTCACCCA TTTGGATAAA CGTAGCCTGC CTGCTCTGAC AAACATCATC AAGATCTTGA
361GGCATGACAT TGGTGCCACT GTCCATGAGC TTTCACGAGA TAAGAAGAAA GACACAGTGC
421CCTGGTTCCC AAGAACCATT CAAGAGCTGG ACAGATTTGC CAATCAGATT CTCAGCTATG
481GAGCGGAACT GGATGCTGAC CACCCTGGTT TTAAAGATCC TGTGTACCGT GCAAGACGGA
541AGCAGTTTGC TGACATTGCC TACAACTACC GCCATGGGCA GCCCATCCCT CGAGTGGAAT
601ACATGGAGGA AGGAAAGAAA ACATGGGGCA CAGTGTTCAA GACTCTGAAG TCCTTGTATA
661AAACCCATGC TTGCTATGAG TACAATCACA TTTTTCCACT TCTTGAAAAG TACTGTGGCT
721TCCATGAAGA TAACATTCCC CAGCTGGAAG ACGTTTCTCA GTTCCTGCAG ACTTGCACTG
781GTTTCCGCCT CCGACCTGTG GCTGGCCTGC TTTCCTCTCG GGATTTCTTG GGTGGCCTGG
841CCTTCCGAGT CTTCCACTGC ACACAGTACA TCAGACATGG ATCCAAGCCC ATGTATACCC
901CCGAACCTGA CATCTGCCAT GAGCTGTTGG GACATGTGCC CTTGTTTTCA GATCGCAGCT
961TTGCCCAGTT TTCCCAGGAA ATTGGCCTTG CCTCTCTGGG TGCACCTGAT GAATACATTG
1021AAAAGCTCGC CACAATTTAC TGGTTTACTG TGGAGTTTGG GCTCTGCAAA CAAGGAGACT
1081CCATAAAGGC ATATGGTGCT GGGCTCCTGT CATCCTTTGG TGAATTACAG TACTGCTTAT
1141CAGAGAAGCC AAAGCTTCTC CCCCTGGAGC TGGAGAAGAC AGCCATCCAA AATTACACTG
1201TCACGGAGTT CCAGCCCCTC TATTACGTGG CAGAGAGTTT TAATGATGCC AAGGAGAAAG
1261TAAGGAACTT TGCTGCCACA ATACCTCGGC CCTTCTCAGT TCGCTACGAC CCATACACCC
1321AAAGGATTGA GGTCTTGGAC AATACCCAGC AGCTTAAGAT TTTGGCTGAT TCCATTAACA
1381GTGAAATTGG AATCCTTTGC AGTGCCCTCC AGAAAATAAA GTACCCAACT TTCTTGTACA
1441AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)