Construct: ORF ccsbBroadEn_06705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014783.1_s300c1, BRDN0000386527
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE9A (5152)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001567.1 | 99.8% | 100% | 9C>G;1311T>C;1317A>C |
| 2 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001574.1 | 94.9% | 95.1% | (many diffs) |
| 3 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001571.1 | 92.1% | 91.9% | (many diffs) |
| 4 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001570.1 | 92% | 93% | (many diffs) |
| 5 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001578.1 | 91.9% | 85.9% | (many diffs) |
| 6 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001583.1 | 90.8% | 85.4% | (many diffs) |
| 7 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_002606.3 | 89.7% | 89.8% | (many diffs) |
| 8 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001315533.1 | 89.2% | 84.3% | (many diffs) |
| 9 | human | 5152 | PDE9A | phosphodiesterase 9A | XM_024452084.1 | 88.3% | 77.4% | (many diffs) |
| 10 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001581.1 | 87.3% | 77.9% | (many diffs) |
| 11 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001568.1 | 87.1% | 86.6% | (many diffs) |
| 12 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001569.1 | 87% | 85.2% | (many diffs) |
| 13 | human | 5152 | PDE9A | phosphodiesterase 9A | XM_011529598.2 | 86.6% | 76% | (many diffs) |
| 14 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001577.1 | 85.9% | 85.7% | (many diffs) |
| 15 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001582.1 | 82.8% | 82.6% | (many diffs) |
| 16 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001575.1 | 80.8% | 80.4% | (many diffs) |
| 17 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001572.1 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 18 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001573.1 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 19 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001579.1 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 20 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001584.2 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 21 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001585.1 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 22 | human | 5152 | PDE9A | phosphodiesterase 9A | XM_017028366.1 | 72.2% | 72.4% | 0_1ins441;870T>C;876A>C |
| 23 | human | 5152 | PDE9A | phosphodiesterase 9A | XM_011529600.2 | 71.6% | 66.9% | (many diffs) |
| 24 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001576.1 | 70.3% | 70.3% | (many diffs) |
| 25 | human | 5152 | PDE9A | phosphodiesterase 9A | NM_001001580.1 | 70.3% | 70.3% | (many diffs) |
| 26 | human | 5152 | PDE9A | phosphodiesterase 9A | XM_017028367.1 | 41.7% | 35.4% | (many diffs) |
| 27 | mouse | 18585 | Pde9a | phosphodiesterase 9A | NM_001163748.1 | 86.7% | 92.1% | (many diffs) |
| 28 | mouse | 18585 | Pde9a | phosphodiesterase 9A | NM_008804.4 | 86.7% | 92.1% | (many diffs) |
| 29 | mouse | 18585 | Pde9a | phosphodiesterase 9A | XR_001782057.1 | 63.6% | (many diffs) | |
| 30 | mouse | 18585 | Pde9a | phosphodiesterase 9A | XR_001782058.1 | 63.5% | (many diffs) | |
| 31 | mouse | 18585 | Pde9a | phosphodiesterase 9A | XM_006523821.2 | 62.6% | 65.6% | (many diffs) |
| 32 | mouse | 18585 | Pde9a | phosphodiesterase 9A | XM_017317319.1 | 62.6% | 65.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG ATCGGGCTCC TCCAGCTACC GGCCCAAGGC CATCTACCTG GACATCGATG 121 GACGCATTCA GAAGGTAATC TTCAGCAAGT ACTGCAACTC CAGCGACATC ATGGACCTGT 181 TCTGCATCGC CACCGGCCTG CCTCGGAACA CGACCATCTC CCTGCTGACC ACCGACGACG 241 CCATGGTCTC CATCGACCCC ACCATGCCCG CGAATTCAGA ACGCACTCCG TACAAAGTGA 301 GACCTGTGGC CATCAAGCAA CTCTCCGAGA GAGAAGAATT AATCCAGAGC GTGCTGGCGC 361 AGGTTGCAGA GCAGTTCTCA AGAGCATTCA AAATCAATGA ACTGAAAGCT GAAGTTGCAA 421 ATCACTTGGC TGTCCTAGAG AAACGCGTGG AATTGGAAGG ACTAAAAGTG GTGGAGATTG 481 AGAAATGCAA GAGTGACATT AAGAAGATGA GGGAGGAGCT GGCGGCCAGA AGCAGCAGGA 541 CCAACTGCCC CTGTAAGTAC AGTTTTTTGG ATAACCACAA GAAGTTGACT CCTCGACGCG 601 ATGTTCCCAC TTACCCCAAG TACCTGCTCT CTCCAGAGAC CATCGAGGCC CTGCGGAAGC 661 CGACCTTTGA CGTCTGGCTT TGGGAGCCCA ATGAGATGCT GAGCTGCCTG GAGCACATGT 721 ACCACGACCT CGGGCTGGTC AGGGACTTCA GCATCAACCC TGTCACCCTC AGGAGGTGGC 781 TGTTCTGCGT CCACGACAAC TACAGAAACA ACCCCTTCCA CAACTTCCGG CACTGCTTCT 841 GCGTGGCCCA GATGATGTAC AGCATGGTCT GGCTCTGCAG TCTCCAGGAG AAGTTCTCAC 901 AAACGGATAT CCTGATCCTA ATGACAGCGG CCATCTGCCA CGATCTGGAC CATCCCGGCT 961 ACAACAACAC GTACCAGATC AATGCCCGCA CAGAGCTGGC GGTCCGCTAC AATGACATCT 1021 CACCGCTGGA GAACCACCAC TGCGCCGTGG CCTTCCAGAT CCTCGCCGAG CCTGAGTGCA 1081 ACATCTTCTC CAACATCCCA CCTGATGGGT TCAAGCAGAT CCGACAGGGA ATGATCACAT 1141 TAATCTTGGC CACTGACATG GCAAGACATG CAGAAATTAT GGATTCTTTC AAAGAGAAAA 1201 TGGAGAATTT TGACTACAGC AACGAGGAGC ACATGACCCT GCTGAAGATG ATTTTGATAA 1261 AATGCTGTGA TATCTCTAAC GAGGTCCGTC CAATGGAAGT CGCAGAGCCT TGGGTGGACT 1321 GTTTATTAGA GGAATATTTT ATGCAGAGCG ACCGTGAGAA GTCAGAAGGC CTTCCCGTGG 1381 CCCCGTTCAT GGACCGAGAC AAAGTGACCA AGGCCACAGC CCAGATTGGG TTCATCAAGT 1441 TTGTCCTGAT CCCAATGTTT GAAACAGTGA CCAAGCTCTT CCCCATGGTT GAGGAGATCA 1501 TGCTGCAGCC ACTTTGGGAA TCCCGAGATC GCTACGAGGA GCTGAAGCGG ATAGATGACG 1561 CCATGAAAGA GTTACAGAAG AAGACTGACA GCTTGACGTC TGGGGCCACC GAGAAGTCCA 1621 GAGAGAGAAG CAGAGATGTG AAAAACAGTG AAGGAGACTG TGCCAGCCCA ACTTTCTTGT 1681 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c