Transcript: Human NM_001001584.2

Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 19, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
PDE9A (5152)
Length:
1853
CDS:
435..1595

Additional Resources:

NCBI RefSeq record:
NM_001001584.2
NBCI Gene record:
PDE9A (5152)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001001584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048894 CGGATAGATGACGCCATGAAA pLKO.1 1476 CDS 100% 5.625 7.875 N PDE9A n/a
2 TRCN0000048896 CGTCCACGACAACTACAGAAA pLKO.1 716 CDS 100% 4.950 6.930 N PDE9A n/a
3 TRCN0000174079 CGTCCACGACAACTACAGAAA pLKO.1 716 CDS 100% 4.950 6.930 N PDE9A n/a
4 TRCN0000048895 GCGTGGAATTGGAAGGACTAA pLKO.1 373 5UTR 100% 4.950 6.930 N PDE9A n/a
5 TRCN0000048897 CGCCATGAAAGAGTTACAGAA pLKO.1 1487 CDS 100% 4.950 3.960 N PDE9A n/a
6 TRCN0000421675 ACAACACGTACCAGATCAATG pLKO_005 892 CDS 100% 10.800 7.560 N PDE9A n/a
7 TRCN0000048893 TGGCACCACAAGACCATGTTT pLKO.1 1682 3UTR 100% 5.625 3.938 N PDE9A n/a
8 TRCN0000418805 GAAATGCAAGAGTGACATTAA pLKO_005 410 5UTR 100% 13.200 7.920 N PDE9A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001001584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06705 pDONR223 100% 72.2% 72.4% None 0_1ins441;870T>C;876A>C n/a
2 ccsbBroad304_06705 pLX_304 0% 72.2% 72.4% V5 0_1ins441;870T>C;876A>C n/a
3 TRCN0000473983 GAACATACGTAAATTATCTCATAT pLX_317 31.7% 72.2% 72.4% V5 0_1ins441;870T>C;876A>C n/a
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