Construct: ORF ccsbBroadEn_06846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011125.1_s300c1, BRDN0000385463
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RB1 (5925)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5925 | RB1 | RB transcriptional corepres... | NM_000321.2 | 100% | 100% | |
| 2 | human | 5925 | RB1 | RB transcriptional corepres... | XM_011535171.2 | 90.5% | 90.5% | 1_1delAins262 |
| 3 | mouse | 19645 | Rb1 | RB transcriptional corepres... | NM_009029.2 | 88.1% | 90.6% | (many diffs) |
| 4 | mouse | 19645 | Rb1 | RB transcriptional corepres... | XR_383150.3 | 78.9% | (many diffs) | |
| 5 | mouse | 19645 | Rb1 | RB transcriptional corepres... | XM_006518731.3 | 55.5% | 53.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2850
- ORF length:
- 2784
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC GCCCAAAACC CCCCGAAAAA CGGCCGCCac cgccgcCGCT GCCGCCGCGG 121 AACCCCCGGC ACCGCCGCCG CCGCCCCCTC CTGAGGAGGA CCCAGAGCAG GACAGCGGCC 181 CGGAGGACCT GCCTCTCGTC AGGCTTGAGT TTGAAGAAAC AGAAGAACCT GATTTTACTG 241 CATTATGTCA GAAATTAAAG ATACCAGATC ATGTCAGAGA GAGAGCTTGG TTAACTTGGG 301 AGAAAGTTTC ATCTGTGGAT GGAGTATTGG GAGGTTATAT TCAAAAGAAA AAGGAACTGT 361 GGGGAATCTG TATCTTTATT GCAGCAGTTG ACCTAGATGA GATGTCGTTC ACTTTTACTG 421 AGCTACAGAA AAACATAGAA ATCAGTGTCC ATAAATTCTT TAACTTACTA AAAGAAATTG 481 ATACCAGTAC CAAAGTTGAT AATGCTATGT CAAGACTGTT GAAGAAGTAT GATGTATTGT 541 TTGCACTCTT CAGCAAATTG GAAAGGACAT GTGAACTTAT ATATTTGACA CAACCCAGCA 601 GTTCGATATC TACTGAAATA AATTCTGCAT TGGTGCTAAA AGTTTCTTGG ATCACATTTT 661 TATTAGCTAA AGGGGAAGTA TTACAAATGG AAGATGATCT GGTGATTTCA TTTCAGTTAA 721 TGCTATGTGT CCTTGACTAT TTTATTAAAC TCTCACCTCC CATGTTGCTC AAAGAACCAT 781 ATAAAACAGC TGTTATACCC ATTAATGGTT CACCTCGAAC ACCCAGGCGA GGTCAGAACA 841 GGAGTGCACG GATAGCAAAA CAACTAGAAA ATGATACAAG AATTATTGAA GTTCTCTGTA 901 AAGAACATGA ATGTAATATA GATGAGGTGA AAAATGTTTA TTTCAAAAAT TTTATACCTT 961 TTATGAATTC TCTTGGACTT GTAACATCTA ATGGACTTCC AGAGGTTGAA AATCTTTCTA 1021 AACGATACGA AGAAATTTAT CTTAAAAATA AAGATCTAGA TGCAAGATTA TTTTTGGATC 1081 ATGATAAAAC TCTTCAGACT GATTCTATAG ACAGTTTTGA AACACAGAGA ACACCACGAA 1141 AAAGTAACCT TGATGAAGAG GTGAATGTAA TTCCTCCACA CACTCCAGTT AGGACTGTTA 1201 TGAACACTAT CCAACAATTA ATGATGATTT TAAATTCAGC AAGTGATCAA CCTTCAGAAA 1261 ATCTGATTTC CTATTTTAAC AACTGCACAG TGAATCCAAA AGAAAGTATA CTGAAAAGAG 1321 TGAAGGATAT AGGATACATC TTTAAAGAGA AATTTGCTAA AGCTGTGGGA CAGGGTTGTG 1381 TCGAAATTGG ATCACAGCGA TACAAACTTG GAGTTCGCTT GTATTACCGA GTAATGGAAT 1441 CCATGCTTAA ATCAGAAGAA GAACGATTAT CCATTCAAAA TTTTAGCAAA CTTCTGAATG 1501 ACAACATTTT TCATATGTCT TTATTGGCGT GCGCTCTTGA GGTTGTAATG GCCACATATA 1561 GCAGAAGTAC ATCTCAGAAT CTTGATTCTG GAACAGATTT GTCTTTCCCA TGGATTCTGA 1621 ATGTGCTTAA TTTAAAAGCC TTTGATTTTT ACAAAGTGAT CGAAAGTTTT ATCAAAGCAG 1681 AAGGCAACTT GACAAGAGAA ATGATAAAAC ATTTAGAACG ATGTGAACAT CGAATCATGG 1741 AATCCCTTGC ATGGCTCTCA GATTCACCTT TATTTGATCT TATTAAACAA TCAAAGGACC 1801 GAGAAGGACC AACTGATCAC CTTGAATCTG CTTGTCCTCT TAATCTTCCT CTCCAGAATA 1861 ATCACACTGC AGCAGATATG TATCTTTCTC CTGTAAGATC TCCAAAGAAA AAAGGTTCAA 1921 CTACGCGTGT AAATTCTACT GCAAATGCAG AGACACAAGC AACCTCAGCC TTCCAGACCC 1981 AGAAGCCATT GAAATCTACC TCTCTTTCAC TGTTTTATAA AAAAGTGTAT CGGCTAGCCT 2041 ATCTCCGGCT AAATACACTT TGTGAACGCC TTCTGTCTGA GCACCCAGAA TTAGAACATA 2101 TCATCTGGAC CCTTTTCCAG CACACCCTGC AGAATGAGTA TGAACTCATG AGAGACAGGC 2161 ATTTGGACCA AATTATGATG TGTTCCATGT ATGGCATATG CAAAGTGAAG AATATAGACC 2221 TTAAATTCAA AATCATTGTA ACAGCATACA AGGATCTTCC TCATGCTGTT CAGGAGACAT 2281 TCAAACGTGT TTTGATCAAA GAAGAGGAGT ATGATTCTAT TATAGTATTC TATAACTCGG 2341 TCTTCATGCA GAGACTGAAA ACAAATATTT TGCAGTATGC TTCCACCAGG CCCCCTACCT 2401 TGTCACCAAT ACCTCACATT CCTCGAAGCC CTTACAAGTT TCCTAGTTCA CCCTTACGGA 2461 TTCCTGGAGG GAACATCTAT ATTTCACCCC TGAAGAGTCC ATATAAAATT TCAGAAGGTC 2521 TGCCAACACC AACAAAAATG ACTCCAAGAT CAAGAATCTT AGTATCAATT GGTGAATCAT 2581 TCGGGACTTC TGAGAAGTTC CAGAAAATAA ATCAGATGGT ATGTAACAGC GACCGTGTGC 2641 TCAAAAGAAG TGCTGAAGGA AGCAACCCTC CTAAACCACT GAAAAAACTA CGCTTTGATA 2701 TTGAAGGATC AGATGAAGCA GATGGAAGTA AACATCTCCC AGGAGAGTCC AAATTTCAGC 2761 AGAAACTGGC AGAAATGACT TCTACTCGAA CACGAATGCA AAAGCAGAAA ATGAATGATA 2821 GCATGGATAC CTCAAACAAG GAAGAGAAAT GCCCAACTTT CTTGTACAAA GTtggcatta 2881 taagaaagca ttgcttatca atttgttgca acgaac