Construct: ORF ccsbBroadEn_06965

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001364.1_s300c1, BRDN0000393348
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC12A4 (6560)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6560 SLC12A4 solute carrier family 12 me... NM_005072.5 99.9% 100% 2881C>A
2 human 6560 SLC12A4 solute carrier family 12 me... NM_001145961.2 99.4% 99.4% 2720_2721insGGTGGAGGTGGTGGAGAT;2863C>A
3 human 6560 SLC12A4 solute carrier family 12 me... NM_001145963.2 97.8% 96.6% (many diffs)
4 human 6560 SLC12A4 solute carrier family 12 me... NM_001145964.2 96.9% 96.5% (many diffs)
5 human 6560 SLC12A4 solute carrier family 12 me... NM_001145962.1 94.1% 93.4% 1_101del;215_216ins95;2887C>A
6 mouse 20498 Slc12a4 solute carrier family 12, m... NM_009195.3 89.2% 96.2% (many diffs)
7 mouse 20498 Slc12a4 solute carrier family 12, m... NM_001253804.1 89% 96% (many diffs)
8 mouse 20498 Slc12a4 solute carrier family 12, m... XM_006530792.2 84.6% 90% (many diffs)
9 mouse 20498 Slc12a4 solute carrier family 12, m... NR_045594.1 75.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3321
ORF length:
3255
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TCACTTCACC GTGGTGCCAG TGGACGGGCC GAGGCGCGGC GACTATGACA
121ACCTCGAGGG GCTCAGTTGG GTGGACTACG GGGAGCGCGC CGAGCTGGAT GACTCGGACG
181GACATGGCAA CCACAGAGAG AGCAGCCCTT TTCTTTCCCC CTTGGAGGCT TCCAGAGGAA
241TTGACTACTA TGACAGGAAC CTGGCACTGT TTGAGGAAGA GCTGGACATC CGCCCAAAGG
301TATCGTCTCT TCTGGGAAAG CTCGTCAGCT ACACCAACCT CACCCAGGGC GCCAAAGAGC
361ATGAGGAGGC CGAGAGTGGG GAGGGCACCC GCCGGAGGGC AGCCGAGGCA CCCAGCATGG
421GCACCCTCAT GGGGGTGTAC CTGCCCTGCC TGCAGAATAT CTTTGGGGTT ATCCTCTTCC
481TGCGGCTGAC CTGGATGGTG GGCACAGCAG GTGTGCTACA GGCCCTCCTC ATCGTGCTTA
541TCTGCTGCTG TTGTACCCTG CTGACGGCCA TCTCCATGAG TGCCATCGCC ACCAACGGTG
601TGGTTCCAGC TGGGGGCTCC TATTTCATGA TCTCTCGTTC ACTGGGGCCA GAATTTGGAG
661GTGCTGTGGG CCTGTGCTTC TACCTGGGAA CAACATTCGC AGCAGCCATG TACATCCTGG
721GGGCCATCGA GATCTTGCTG ACCTACATTG CCCCACCAGC TGCCATTTTT TACCCATCGG
781GTGCTCATGA CACGTCGAAT GCCACTTTGA ACAATATGCG TGTGTATGGG ACCATTTTCC
841TGACCTTCAT GACCCTGGTG GTGTTTGTGG GGGTCAAGTA TGTGAACAAA TTTGCCTCGC
901TCTTCCTGGC CTGTGTGATC ATCTCCATCC TCTCCATCTA TGCTGGGGGC ATAAAGTCTA
961TATTTGACCC TCCCGTGTTT CCGGTATGCA TGCTGGGCAA CAGGACCCTG TCCCGGGACC
1021AGTTTGACAT CTGTGCCAAG ACAGCTGTAG TGGACAATGA GACAGTGGCC ACCCAGCTAT
1081GGAGTTTCTT CTGCCACAGC CCCAACCTTA CGACCGACTC CTGTGACCCC TACTTCATGC
1141TCAACAATGT GACCGAGATC CCTGGCATCC CCGGGGCAGC TGCTGGTGTG CTCCAGGAAA
1201ACCTGTGGAG CGCCTACCTG GAGAAGGGTG ACATCGTGGA GAAGCATGGG CTGCCCTCCG
1261CAGATGCCCC GAGCCTGAAG GAGAGCCTGC CTCTGTACGT GGTCGCTGAC ATCGCCACAT
1321CCTTCACCGT GCTGGTCGGC ATCTTCTTCC CTTCTGTAAC AGGCATCATG GCTGGCTCAA
1381ACCGCTCTGG GGACCTTCGT GACGCCCAGA AGTCTATCCC TGTGGGGACC ATTCTGGCCA
1441TCATTACAAC TTCCCTCGTG TACTTCAGCA GTGTGGTTCT CTTTGGTGCC TGCATTGAGG
1501GTGTGGTTCT CCGGGACAAG TATGGCGATG GTGTCAGCAG GAACTTGGTG GTGGGCACAC
1561TGGCCTGGCC TTCACCCTGG GTCATCGTCA TCGGCTCCTT CTTTTCAACG TGTGGCGCTG
1621GCCTCCAGAG CCTCACAGGG GCACCACGCC TATTGCAGGC CATTGCCAAG GACAACATCA
1681TCCCCTTCCT CCGGGTGTTT GGCCACGGGA AGGTGAATGG TGAACCCACA TGGGCACTCC
1741TCCTGACGGC ACTCATCGCC GAGCTGGGCA TCCTCATCGC CTCCCTCGAC ATGGTGGCCC
1801CCATCTTATC CATGTTCTTT CTGATGTGCT ACCTGTTCGT GAACCTCGCC TGTGCGGTGC
1861AGACACTCCT GAGGACCCCC AACTGGCGGC CCCGGTTCAA GTACTATCAC TGGGCGCTGT
1921CCTTCCTGGG CATGAGTCTC TGCCTGGCCC TTATGTTTGT CTCCTCCTGG TACTATGCCC
1981TGGTGGCCAT GCTCATCGCC GGCATGATCT ACAAATACAT CGAGTACCAA GGGGCTGAGA
2041AGGAGTGGGG TGACGGGATC CGAGGCCTGT CCCTGAGCGC TGCCCGCTAC GCGCTGTTGC
2101GGCTGGAGGA GGGGCCTCCT CACACCAAGA ACTGGCGGCC GCAGCTGCTG GTGCTGCTGA
2161AGCTGGACGA GGACCTCCAC GTGAAGTACC CGCGGCTCCT CACCTTCGCC TCCCAGCTCA
2221AGGCTGGCAA GGGCCTGACC ATTGTTGGTT CTGTCATCCA GGGGAGCTTC TTGGAGAGCT
2281ATGGCGAGGC TCAGGCCGCC GAGCAGACCA TCAAGAACAT GATGGAAATT GAGAAGGTGA
2341AGGGCTTCTG CCAGGTGGTG GTGGCCAGCA AGGTGCGGGA GGGGCTGGCC CACCTCATCC
2401AGTCCTGTGG CCTGGGAGGC ATGCGGCATA ACTCCGTGGT GCTGGGCTGG CCCTACGGCT
2461GGCGACAGAG CGAGGACCCC CGTGCCTGGA AGACCTTCAT TGACACCGTG CGCTGCACTA
2521CGGCTGCCCA CCTGGCCCTG CTCGTGCCCA AGAACATCGC CTTCTACCCC AGCAACCACG
2581AGCGCTACCT GGAGGGCCAC ATAGACGTGT GGTGGATCGT GCACGATGGT GGCATGCTCA
2641TGCTTCTGCC CTTCCTGCTG CGCCAGCATA AGGTCTGGAG GAAGTGCCGG ATGCGCATCT
2701TCACAGTGGC CCAGATGGAT GACAACAGCA TCCAGATGAA GAAGGACCTG GCTGTCTTTC
2761TGTACCATCT GCGCCTTGAG GCCGAGGTGG AGGTGGTGGA GATGCATAAC AGTGACATCT
2821CTGCATACAC CTACGAGCGG ACGCTGATGA TGGAGCAGCG GTCGCAGATG CTGCGGCAGA
2881TGAGACTGAC CAAGACTGAG CGGGAGCGAG AAGCCCAGCT GGTCAAGGAT CGGCACTCGG
2941CCCTGAGGCT GGAGAGCCTG TACTCGGACG AGGAAGATGA GTCTGCAGTG GGGGCTGACA
3001AGATCCAGAT GACGTGGACC AGGGACAAGT ACATGACTGA GACCTGGGAC CCCAGCCATG
3061CCCCTGACAA TTTCCGGGAG CTGGTGCACA TTAAGCCGGA CCAATCCAAT GTGCGGCGCA
3121TGCACACTGC TGTGAAGCTC AATGAAGTCA TTGTCACGCG CTCCCACGAC GCCCGCCTGG
3181TTCTCCTAAA CATGCCTGGC CCACCCAGGA ACAGTGAGGG CGACGAGAAC TACATGGAGT
3241TCCTCGAGGT GCTGACCGAG GGCCTTGAGC GGGTGCTGTT GGTGCGCGGT GGTGGCCGTG
3301AAGTCATCAC CATCTACTCC TGCCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
3361attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)