Construct: ORF ccsbBroadEn_06999

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010285.1_s300c1, BRDN0000390696
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRPK2 (6733)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6733 SRPK2 SRSF protein kinase 2 NM_001278273.1 99.8% 99.7% 410C>G;1599T>C;1918A>G
2 human 6733 SRPK2 SRSF protein kinase 2 NM_001350738.1 99.8% 99.7% 410C>G;1599T>C;1918A>G
3 human 6733 SRPK2 SRSF protein kinase 2 NM_182691.2 99.8% 99.7% 410C>G;1599T>C;1918A>G
4 human 6733 SRPK2 SRSF protein kinase 2 NM_001350739.1 97.8% 98.2% (many diffs)
5 human 6733 SRPK2 SRSF protein kinase 2 NM_182692.3 97.3% 96.4% (many diffs)
6 human 6733 SRPK2 SRSF protein kinase 2 NM_001350743.1 95.5% 95.4% (many diffs)
7 human 6733 SRPK2 SRSF protein kinase 2 NM_001350744.1 95.5% 95.4% (many diffs)
8 human 6733 SRPK2 SRSF protein kinase 2 XM_006716098.3 95.5% 95.4% (many diffs)
9 human 6733 SRPK2 SRSF protein kinase 2 XM_011516538.3 95.5% 95.4% (many diffs)
10 human 6733 SRPK2 SRSF protein kinase 2 NM_001350742.1 94.7% 94.6% (many diffs)
11 human 6733 SRPK2 SRSF protein kinase 2 NM_001350741.1 93.2% 92.3% (many diffs)
12 human 6733 SRPK2 SRSF protein kinase 2 NM_001350745.1 93.1% 92.8% (many diffs)
13 human 6733 SRPK2 SRSF protein kinase 2 XM_011516536.3 92.9% 92.4% (many diffs)
14 human 6733 SRPK2 SRSF protein kinase 2 XM_017012561.2 92.8% 93.2% (many diffs)
15 human 6733 SRPK2 SRSF protein kinase 2 NM_001350740.1 92.3% 91.5% (many diffs)
16 human 6733 SRPK2 SRSF protein kinase 2 XM_024446893.1 90.8% 90.7% (many diffs)
17 human 6733 SRPK2 SRSF protein kinase 2 XM_024446895.1 90.5% 90.2% (many diffs)
18 human 6733 SRPK2 SRSF protein kinase 2 XM_024446894.1 88.6% 87.8% (many diffs)
19 human 6733 SRPK2 SRSF protein kinase 2 NM_001350746.1 80% 78.1% (many diffs)
20 human 6733 SRPK2 SRSF protein kinase 2 XR_001744860.2 60.4% (many diffs)
21 human 6733 SRPK2 SRSF protein kinase 2 XR_001744863.2 49.6% (many diffs)
22 human 6733 SRPK2 SRSF protein kinase 2 XR_001744861.2 48.4% (many diffs)
23 human 6733 SRPK2 SRSF protein kinase 2 XR_001744862.2 45.6% (many diffs)
24 human 6733 SRPK2 SRSF protein kinase 2 XR_001744859.2 43.7% (many diffs)
25 mouse 20817 Srpk2 serine/arginine-rich protei... NM_009274.2 89.7% 93.9% (many diffs)
26 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320769.1 89.7% 93.9% (many diffs)
27 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535661.3 87.2% 90.7% (many diffs)
28 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249755.2 85.8% 89.8% (many diffs)
29 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249756.2 85.8% 89.8% (many diffs)
30 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249757.2 85.8% 89.8% (many diffs)
31 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535660.3 85.1% 89.1% (many diffs)
32 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320767.1 84% 87.8% (many diffs)
33 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249753.2 83.5% 86.8% (many diffs)
34 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249754.2 83.4% 87.7% (many diffs)
35 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320768.1 82.3% 86.5% (many diffs)
36 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249751.2 81.6% 85.4% (many diffs)
37 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249752.2 80.5% 84.2% (many diffs)
38 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320771.1 76.4% 79.8% (many diffs)
39 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249758.2 73.1% 76.3% (many diffs)
40 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320770.1 73.1% 76.3% (many diffs)
41 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320774.1 69.2% 70.2% (many diffs)
42 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320776.1 68% 68.9% (many diffs)
43 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320775.1 67.9% 70.8% (many diffs)
44 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320777.1 66.7% 69.5% (many diffs)
45 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320773.1 66.2% 67.2% (many diffs)
46 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320772.1 65.1% 66% (many diffs)
47 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249759.2 65% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2130
ORF length:
2064
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC AGTTAACTCT GAGAAGTCGT CCTCTTCAGA AAGGCCGGAG CCTCAACAGA
121AAGCTCCTTT AGTTCCTCCT CCTCCACCGC CACCACCACC ACCACCGCCA CCTTTGCCAG
181ACCCCACACC CCCGGAGCCA GAGGAGGAGA TCCTGGGATC AGATGATGAG GAGCAAGAGG
241ACCCTGCGGA CTACTGCAAA GGTGGATATC ATCCAGTGAA AATTGGAGAC CTCTTCAATG
301GCCGGTATCA TGTTATTAGA AAGCTTGGAT GGGGGCACTT CTCTACTGTC TGGCTGTGCT
361GGGATATGCA GGGGAAAAGA TTTGTTGCAA TGAAAGTTGT AAAAAGTGCC CAGCATTATA
421CGGAGACAGC CTTGGATGAA ATAAAATTGC TCAAATGTGT TCGAGAAAGT GATCGCAGTG
481ACCCAAACAA AGACATGGTG GTCCAGCTCA TTGACGACTT CAAGATTTCA GGCATGAATG
541GGATACATGT CTGCATGGTC TTCGAAGTAC TTGGCCACCA TCTCCTCAAG TGGATCATCA
601AATCCAACTA TCAAGGCCTC CCAGTACGTT GTGTGAAGAG TATCATTCGA CAGGTCCTTC
661AAGGGTTAGA TTACTTACAC AGTAAGTGCA AGATCATTCA TACTGACATA AAGCCGGAAA
721ATATCTTGAT GTGTGTGGAT GATGCATATG TGAGAAGAAT GGCAGCTGAG GCCACTGAGT
781GGCAGAAAGC AGGTGCTCCT CCTCCTTCAG GGTCTGCAGT GAGTACGGCT CCACAGCAGA
841AACCTATAGG AAAAATATCT AAAAACAAAA AGAAAAAACT GAAAAAGAAA CAGAAGAGGC
901AGGCTGAGTT ATTGGAGAAG CGCCTGCAGG AGATAGAAGA ATTGGAGCGA GAAGCTGAAA
961GGAAAATAAT AGAAGAAAAC ATCACCTCAG CTGCACCTTC CAATGACCAG GATGGCGAAT
1021ACTGCCCAGA GGTGAAACTA AAAACAACAG GATTAGAGGA GGCGGCTGAG GCAGAGACTG
1081CAAAGGACAA TGGTGAAGCT GAGGACCAGG AAGAGAAAGA AGATGCTGAG AAAGAAAACA
1141TTGAAAAAGA TGAAGATGAT GTAGATCAGG AACTTGCGAA CATAGACCCT ACGTGGATAG
1201AATCACCTAA AACCAATGGC CATATTGAGA ATGGCCCATT CTCACTGGAG CAGCAACTGG
1261ACGATGAAGA TGATGATGAA GAAGACTGCC CAAATCCTGA GGAATATAAT CTTGATGAGC
1321CAAATGCAGA AAGTGATTAC ACATATAGCA GCTCCTATGA ACAATTCAAT GGTGAATTGC
1381CAAATGGACG ACATAAAATT CCCGAGTCAC AGTTCCCAGA GTTTTCCACC TCGTTGTTCT
1441CTGGATCCTT AGAACCTGTG GCCTGCGGCT CTGTGCTTTC TGAGGGATCA CCACTTACTG
1501AGCAAGAGGA GAGCAGTCCA TCCCATGACA GAAGCAGAAC GGTTTCAGCC TCCAGTACTG
1561GGGATTTGCC AAAAGCAAAA ACCCGGGCAG CTGACTTGTT GGTGAATCCC CTGGATCCGC
1621GGAATGCAGA TAAAATTAGA GTAAAAATTG CTGACCTGGG AAACGCTTGT TGGGTGCATA
1681AACACTTCAC GGAAGACATC CAGACGCGTC AGTACCGCTC CATAGAGGTT TTAATAGGAG
1741CGGGGTACAG CACCCCTGCG GACATCTGGA GCACGGCGTG TATGGCATTT GAGCTGGCAA
1801CGGGAGATTA TTTGTTTGAA CCACATTCTG GGGAAGACTA TTCCAGAGAC GAAGACCACA
1861TAGCCCACAT CATAGAGCTG CTAGGCAGTA TTCCAAGGCA CTTTGCTCTA TCTGGAAAAT
1921ATTCTCGGGA ATTCTTCAAT CGCAGAGGAG AACTGCGACA CATCACCAAG CTGAAGCCCT
1981GGGGCCTCTT TGATGTACTT GTGGAAAAGT ATGGCTGGCC CCATGAAGAT GCTGCACAGT
2041TTACAGATTT CCTGATCCCG ATGTTAGAAA TGGTTCCAGA AAAACGAGCC TCAGCTGGCG
2101AATGCCTTCG GCATCCTTGG TTGAATTCTT ACCCAACTTT CTTGTACAAA GTtggcatta
2161taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)