Construct: ORF ccsbBroadEn_07101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004379.1_s300c1, BRDN0000457422
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSC2 (7249)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001114382.2 | 99.9% | 99.7% | (many diffs) |
| 2 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_000548.5 | 98.6% | 98.5% | (many diffs) |
| 3 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_017023615.1 | 98.5% | 98.4% | (many diffs) |
| 4 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_011522636.2 | 97.6% | 97.5% | (many diffs) |
| 5 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_011522637.2 | 97.6% | 97.4% | (many diffs) |
| 6 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001363528.1 | 97.5% | 97.3% | (many diffs) |
| 7 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001077183.2 | 97.4% | 97.3% | (many diffs) |
| 8 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001318832.1 | 96.8% | 96.7% | (many diffs) |
| 9 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_021055.2 | 96.2% | 96.1% | (many diffs) |
| 10 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001370404.1 | 96.2% | 96% | (many diffs) |
| 11 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001370405.1 | 96% | 95.9% | (many diffs) |
| 12 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_024450413.1 | 95.8% | 95.7% | (many diffs) |
| 13 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001318827.1 | 95.4% | 95.2% | (many diffs) |
| 14 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_011522639.2 | 95.3% | 95.1% | (many diffs) |
| 15 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_011522640.2 | 95.2% | 95.1% | (many diffs) |
| 16 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001318829.1 | 94.7% | 94.6% | (many diffs) |
| 17 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_011522638.2 | 91.1% | 90.9% | (many diffs) |
| 18 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_017023617.1 | 90.8% | 90.6% | (many diffs) |
| 19 | human | 7249 | TSC2 | TSC complex subunit 2 | NM_001318831.1 | 85.1% | 85% | (many diffs) |
| 20 | human | 7249 | TSC2 | TSC complex subunit 2 | XM_017023618.1 | 73.1% | 73% | (many diffs) |
| 21 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001286714.1 | 84.6% | 91.1% | (many diffs) |
| 22 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_011647.3 | 84.6% | 91.1% | (many diffs) |
| 23 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_006524040.2 | 84.6% | 91.1% | (many diffs) |
| 24 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317387.1 | 84.5% | 91.1% | (many diffs) |
| 25 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001286713.1 | 83.5% | 90% | (many diffs) |
| 26 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317384.1 | 83.5% | 90% | (many diffs) |
| 27 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317386.1 | 83.5% | 89.9% | (many diffs) |
| 28 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317385.1 | 83.5% | 89.9% | (many diffs) |
| 29 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001286720.1 | 82.5% | 88% | (many diffs) |
| 30 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001039363.2 | 82.3% | 88.8% | (many diffs) |
| 31 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001286716.1 | 82.3% | 88.8% | (many diffs) |
| 32 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | NM_001286718.1 | 82.3% | 88.8% | (many diffs) |
| 33 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_006524044.2 | 82.3% | 88.8% | (many diffs) |
| 34 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317392.1 | 82.3% | 88.7% | (many diffs) |
| 35 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317388.1 | 81.3% | 87.7% | (many diffs) |
| 36 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317389.1 | 81.3% | 87.6% | (many diffs) |
| 37 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317390.1 | 80.6% | 86.9% | (many diffs) |
| 38 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317391.1 | 80.6% | 86.9% | (many diffs) |
| 39 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XR_001782062.1 | 79.8% | (many diffs) | |
| 40 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XM_017317393.1 | 79.4% | 85.8% | (many diffs) |
| 41 | mouse | 22084 | Tsc2 | tuberous sclerosis 2 | XR_001782063.1 | 77.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5421
- ORF length:
- 5352
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCCAAACCA ACAAGCAAAG ATTCAGGCTT GAAGGAGAAG TTTAAGATTC 121 TGTTGGGACT GGGAACACCG AGGCCAAATC CCAGGTCTGC AGAGGGTAAA CAGACGGAGT 181 TTATCATCAC CGCGGAAATA CTGAGAGAAC TGAGCATGGA ATGTGGCCTC AACAATCGCA 241 TCCGGATGAT AGGGCAGATT TGTGAAGTCG CAAAAACCAA GAAATTTGAA GAGCACGCAG 301 TGGAAGCACT CTGGAAGGCG GTCGCGGATC TGTTGCAGCC GGAGCGGCCG CTGGAGGCCC 361 GGCACGCGGT GCTGGCTCTG CTGAAGGCCA TCGTGCAGGG GCAGGGCGAG CGTTTGGGGG 421 TCCTCAGAGC CCTCTTCTTT AAGGTCATCA AGGATTACCC TTCCAACGAA GACCTTCACG 481 AAAGGCTGGA GGTTTTCAAG GCCCTCACAG ACAATGGGAG ACACATCACC TACTTGGAGG 541 AAGAGCTGGC TGACTTTGTC CTGCAGTGGA TGGATGTTGG CTTGTCCTCG GAATTCCTTC 601 TGGTGCTGGT GAACTTGGTC AAATTCAATA GCTGTTACCT CGACGAGTAC ATCGCAAGGA 661 TGGTTCAGAT GATCTGTCTG CTGTGCGTCC GGACCGTGTC CTCTGTGGAC ATAGAGGTCT 721 CCCTGCAGGT GCTGGACGCC GTGGTCTGCT ACAACTGCCT GCCGGCTGAG AGCCTCCCGC 781 TGTTCATCGT TACCCTCTGT CGCACCATCA ACGTCAAGGA GCTCTGCGAG CCTTGCTGGA 841 AGCTGATGCG GAACCTCCTT GGCACCCACC TGGGCCACAG CGCCATCTAC AACATGTGCC 901 ACCTCATGGA GGACAGAGCC TACATGGAGG ACGCGCCCCT GCTGAGAGGA GCCGTGTTTT 961 TTGTGGGCAT GGCTCTCTGG GGAGCCCACC GGCTCTATTC TCTCAGGAAC TCGCCGACAT 1021 CTGTGTTGCC ATCATTTTAC CAGGCCATGG CATGTCCGAA CGAGGTGGTG TCCTATGAGA 1081 TCGTCCTGTC CATCACCAGG CTCATCAAGA AGTATAGGAA GGAGCTCCAG GTGGTGGCGT 1141 GGGACATTCT GCTGAACATC ATCGAACGGC TCCTTCAGCA GCTCCAGACC TTGGACAGCC 1201 CGGAGCTCAG GACCATCGTC CATGACCTGT TGACCACGGT GGAGGAGCTG TGTGACCAGA 1261 ACGAGTTCCA CGGGTCTCAG GAGAGATACT TTGAACTGGT GGAGAGATGT GCGGACCAGA 1321 GGCCTGAGTC CTCCCTCCTG AACCTGATCT CCTATAGAGC GCAGTCCATC CACCCGGCCA 1381 AGGACGGCTG GATTCAGAAC CTGCAGGCGC TGATGGAGAG ATTCTTCAGG AGCGAGTCCC 1441 GAGGCGCCGT GCGCATCAAG GTGCTGGACG TGCTGTCCTT TGTGCTGCTC ATCAACAGGC 1501 AGTTCTATGA GGAGGAGCTG ATTAACTCAG TGGTCATCTC GCAGCTCTCC CACATCCCCG 1561 AGGATAAAGA CCACCAGGTC CGAAAGCTGG CCACCCAGTT GCTGGTGGAC CTGGCAGAGG 1621 GCTGCCACAC ACACCACTTC AACAGCCTGC TGGACATCAT CGAGAAGGTG ATGGCCCGCT 1681 CCCTCTCCCC ACCCCCGGAG CTGGAAGAAA GGGATGTGGC CGCATACTCG GCCTCCTTGG 1741 AGGATGTGAA GACAGCCGTC CTGGGGCTTC TGGTCATCCT TCAGACCAAG CTGTACACCC 1801 TGCCTGCAAG CCACGCCACG CGTGTGTATG AGATGCTGGT CAGCCACATT CAGCTCCACT 1861 ACAAGCACAG CTACACCCTG CCAATCGCGA GCAGCATCCG GCTGCAGGCC TTTGACTTCC 1921 TGTTGCTGCT GCGGGCCGAC TCACTGCACC GCCTGGGCCT GCCCAACAAG GATGGAGTCG 1981 TGCGGTTCAG CCCCTACTGC GTCTGCGACT ACATGGAGCC AGAGAGAGGC TCTGAGAAGA 2041 AGACCAGCGG CCCCCTTTCT CCTCCCACAG GGCCTCCTGG CCCGGCGCCT GCAGGCCCCG 2101 CCGTGCGGCT GGGGTCCGTG CCCTACTCCC TGCTCTTCCG CGTCCTGCTG CAGTGCTTGA 2161 AGCAGGAGTC TGACTGGGAG GTGCTGAAGC TGGTTCTGGG CAGGCTGCCT GAGTCCCTGC 2221 GCTATAAAGT GCTCATCTTT ACTTCCCCTT GCAGTGTGGA CCAGCTGTGC TCTGCTCTCT 2281 GCTCCATGCT TTCAGGCCCA AAGACACTGG AGCGGCTCCG AGGCGCCCCA GAAGGCTTCT 2341 CCAGAACTGA CTTGCACCTG GCCGTGGTTC CAGTGCTGAC AGCATTAATC TCTTACCATA 2401 ACTACCTGGA CAAAACCAAA CAGCGCGAGA TGGTCTACTG CCTGGAGCAG GGCCTCATCC 2461 ACCGCTGTGC CAGCCAGTGC GTCGTGGCCT TGTCCATCTG CAGCGTGGAG ATGCCTGACA 2521 TCATCATCAA GGCGCTGCCT GTTCTGGTGG TGAAGCTCAC GCACATCTCA GCCACAGCCA 2581 GCATGGCCGT CCCACTGCTG GAGTTCCTGT CCACTCTGGC CAGGCTGCCG CACCTCTACA 2641 GGAACTTTGC CGCGGAGCAG TATGCCAGTG TGTTCGCCAT CTCCCTGCCG TACACCAACC 2701 CCTCCAAGTT TAATCAGTAC ATCGTGTGTC TGGCCCATCA CGTCATAGCC ATGTGGTTCA 2761 TCAGGTGCCG CCTGCCCTTC CGGAAGGATT TTGTCCCTTT CATCACTAAG GGCCTGCGGT 2821 CCAATGTCCT CTTGTCTTTT GATGACACCC CCGAGAAGGA CAGCTTCAGG GCCCGGAGTA 2881 CTAGTCTCAA CGAGAGACCC AAGAGTCTGA GGATAGCCAG ACCCCCCAAA CAAGGCTTGA 2941 ATAACTCTCC ACCCGTGAAA GAATTCAAGG AGAGCTCTGC AGCCGAGGCC TTCCGGTGCC 3001 GCAGCATCAG TGTGTCTGAA CATGTGGTCC GCAGCAGGAT ACAGACGTCC CTCACCAGTG 3061 CCAGCTTGGG GTCTGCAGAT GAGAACTCCG TGGCCCAGGC TGACGATAGC ATGAAAAACC 3121 TCCACCTGGA GCTCACGGAA ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT 3181 TCACGGCTGT CCCGAAGAGG TCTCCTGTGG GCGAGTTCCT CCTAGCGGGT GGCAGGACCA 3241 AAACCTGGCT GGTTGGGAAC AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC 3301 GGTCGTTACT AGGCCTGGAC TCGGGGGAGC TGCAGTCCGG CCCGGAGTCG AGCTCCAGCC 3361 CCGGGGTGCA TGTGAGACAG ACCAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC 3421 AGCAGGTGTC CCGTGGGGCC CGGGATCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC 3481 GAGTTGGCGC CCTGGACGTG CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC 3541 CACGGACTGC ACCAGCCGCG AAACCTGAGA AGGCCTCAGC TGGCACCCGG GTTCCTGTGC 3601 AGGAGAAGAC GAACCTGGCG GCCTATGTGC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC 3661 TGGTCCGGAG GCCCACAGGG AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC 3721 CTTTCTCCTC GGACATCAAC AACATGCCCC TGCAGGAGCT GTCTAACGCC CTCATGGCGG 3781 CTGAGCGCTT CAAGGAGCAC CGGGACACAG CCCTGTACAA GTCACTGTCG GTGCCGGCAG 3841 CCAGCACGGC CAAACCCCCT CCTCTGCCTC GCTCCAACAC AGACTCCGCC GTGGTCATGG 3901 AGGAGGGAAG TCCGGGCGAG GTTCCTGTGC TGGTGGAGCC CCCAGGGTTG GAGGACGTTG 3961 AGGCAGCGCT AGGCATGGAC AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA 4021 GCCAGGAGGA GAAGTCGCTC CACGCGGAGG AGCTGGTTGG CAGGGGCATC CCCATCGAGC 4081 GAGTCGTCTC CTCGGAGGGT GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC 4141 CCCTGAGCAA GTCCAGCTCC TCTCCCGAGC TGCAGACTCT GCAGGACATC CTCGGGGACC 4201 CTGGGGACAA GGCCGACGTG GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG 4261 GGACCCTGGA CGGGGAAAGT GCTGCCTGGT CGGCCTCGGG CGAAGACAGT CGGGGCCAGC 4321 CCGAGGGTCC CTTGCCTTCC AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT 4381 ACACCATCTC CGACTCGGCC CCATCACGCA GGGGCAAGAG AGTAGAGAGG GACGCCTTAA 4441 AGAGCAGAGC CACAGCCTCC AATGCAGAGA AAGTGCCAGG CATCAACCCC AGTTTCGTGT 4501 TCCTGCAGCT CTACCATTCC CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC 4561 CCAATGAGTC ACAGTCCTTT GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG 4621 ACACCCACAA GATCGCCGTC CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA 4681 TCCTGTCCAA TGAGCATGGC TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC 4741 TCATCGAGCT GAAGGACTGC CAGCCGGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG 4801 GTGAGGACGG CCAGTTCACC TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA 4861 TCGCCACCCT GATGCCCACC AAGGACGTGG ACAAGCACCG CTGCGACAAG AAGCGCCACC 4921 TGGGCAACGA CTTTGTGTCC ATTGTCTACA ATGACTCCGG TGAGGACTTC AAGCTTGGCA 4981 CCATCAAGGG CCAGTTCAAC TTTGTCCACG TGATGGTCAC CCCGCTGGAC TACGAGTGCA 5041 ACCTGGTGTC CCTGCAGTGC AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA 5101 AGATCGTGTC TGACCGCAAC CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA 5161 TGGCCTCACA GGTGCATCAT AGCCGCTCCA ACCCCACCGA TATCTACCCC TCCAAGTGGA 5221 TTGCCCGGCT CCGCCACATC AAGCGGCTCC GCCAGCGGAT CTGCGAGGAA GCCGCCTACT 5281 CCAACCCCAG CCTACCTCTG GTGCACCCTC CGTCCCATAG CAAAGCCCCT GCACAGACTC 5341 CAGCCGAGCC CACACCTGGC TATGAGGTGG GCCAGCGGAA GCGCCTCATC TCCTCGGTGG 5401 AGGACTTCAC CGAGTTTGTG TTGCCAACTT TCTTGTACAA AGTTGGcatt ataagaaagc 5461 attgcttatc aatttgttgc aacgaac