Transcript: Mouse XM_017317392.1

PREDICTED: Mus musculus tuberous sclerosis 2 (Tsc2), transcript variant X11, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tsc2 (22084)
Length:
5460
CDS:
114..5336

Additional Resources:

NCBI RefSeq record:
XM_017317392.1
NBCI Gene record:
Tsc2 (22084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017317392.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042725 CCCTTATATCACTAAGGGTTT pLKO.1 2837 CDS 100% 0.405 0.567 N Tsc2 n/a
2 TRCN0000042724 CCTCTCTCTTAAACTTGATAT pLKO.1 1372 CDS 100% 13.200 10.560 N Tsc2 n/a
3 TRCN0000326277 CCTCTCTCTTAAACTTGATAT pLKO_005 1372 CDS 100% 13.200 10.560 N Tsc2 n/a
4 TRCN0000306245 TCGCAAGACTCCGCCACATTA pLKO_005 5137 CDS 100% 13.200 10.560 N Tsc2 n/a
5 TRCN0000306244 CTGTACTCCTACATGAAATAA pLKO_005 5389 3UTR 100% 15.000 10.500 N Tsc2 n/a
6 TRCN0000042726 GCAGCCTATGTGCCTTTGTTA pLKO.1 3528 CDS 100% 5.625 3.938 N Tsc2 n/a
7 TRCN0000042727 CGAGTGTATGAGAGCCTCATT pLKO.1 1863 CDS 100% 4.950 3.465 N Tsc2 n/a
8 TRCN0000326275 CGAGTGTATGAGAGCCTCATT pLKO_005 1863 CDS 100% 4.950 3.465 N Tsc2 n/a
9 TRCN0000042723 GCCCGATATGTGTTCTCCAAT pLKO.1 3069 CDS 100% 4.950 3.465 N Tsc2 n/a
10 TRCN0000326276 GCCCGATATGTGTTCTCCAAT pLKO_005 3069 CDS 100% 4.950 3.465 N Tsc2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017317392.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07101 pDONR223 100% 82.3% 88.7% None (many diffs) n/a
2 ccsbBroad304_07101 pLX_304 12.7% 82.3% 88.7% V5 (many diffs) n/a
3 TRCN0000476551 CGGAATCACTATGTGAGCATTTTA pLX_317 7.2% 82.3% 88.7% V5 (many diffs) n/a
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