Construct: ORF ccsbBroadEn_07194

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001222.1_s300c1, BRDN0000389639
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GLRA3 (8001)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8001 GLRA3 glycine receptor alpha 3 NM_006529.4 99.7% 100% 822A>G;876T>C;1080T>C
2 human 8001 GLRA3 glycine receptor alpha 3 NM_001042543.3 96.6% 96.7% 822A>G;876T>C;1070_1071ins45
3 human 8001 GLRA3 glycine receptor alpha 3 XM_017008630.1 91.1% 91.3% (many diffs)
4 human 8001 GLRA3 glycine receptor alpha 3 XM_011532267.2 66.5% 66.5% (many diffs)
5 human 8001 GLRA3 glycine receptor alpha 3 XM_011532268.2 57.7% 57.9% (many diffs)
6 human 8001 GLRA3 glycine receptor alpha 3 XR_001741328.1 52% (many diffs)
7 human 8001 GLRA3 glycine receptor alpha 3 XR_001741329.1 50% (many diffs)
8 mouse 110304 Glra3 glycine receptor, alpha 3 s... NM_080438.2 86.2% 95.6% (many diffs)
9 mouse 110304 Glra3 glycine receptor, alpha 3 s... XM_006509247.2 83.4% 92.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1458
ORF length:
1392
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CCACGTGAGA CACTTTCGGA CATTAGTTTC GGGATTTTAC TTCTGGGAAG
121CAGCACTGTT ACTCAGTTTG GTTGCCACAA AGGAAACAGA CAGTGCAAGA TCTCGAAGTG
181CTCCAATGTC ACCTTCTGAT TTTCTGGATA AATTAATGGG CAGGACATCA GGATATGATG
241CAAGAATCAG ACCCAATTTT AAAGGCCCTC CAGTTAATGT CACATGCAAC ATATTCATCA
301ACAGCTTTGG CTCTATCGCA GAGACGACCA TGGATTACAG AGTGAATATC TTTCTTCGTC
361AGAAATGGAA TGATCCCCGC CTCGCGTACA GTGAATATCC TGACGACTCT TTAGACCTCG
421ACCCCTCCAT GTTGGACTCC ATTTGGAAAC CTGATTTGTT CTTTGCCAAT GAAAAGGGTG
481CCAACTTTCA TGAAGTCACT ACAGACAACA AATTGCTAAG AATTTTCAAA AATGGAAATG
541TTCTTTATTC AATAAGATTA ACATTAACAC TTTCCTGTCC AATGGATCTC AAGAATTTTC
601CCATGGATGT ACAAACATGT ATAATGCAAC TGGAAAGCTT TGGGTACACA ATGAATGATC
661TCATTTTTGA ATGGCAAGAT GAGGCACCCG TACAAGTGGC AGAAGGACTC ACTTTGCCCC
721AGTTTCTGTT GAAAGAAGAA AAAGATTTAC GATACTGCAC TAAACATTAC AATACAGGAA
781AGTTTACGTG TATAGAAGTG CGATTCCATC TGGAGCGACA AATGGGATAC TATCTGATCC
841AGATGTACAT TCCCAGTCTC CTGATTGTTA TTCTATCCTG GGTTTCGTTC TGGATCAACA
901TGGATGCAGC ACCGGCCAGG GTAGCTCTGG GGATAACCAC CGTGCTAACG ATGACTACAC
961AGAGTTCAGG ATCACGAGCT TCCTTGCCAA AAGTTTCATA TGTCAAAGCT ATTGATATTT
1021GGATGGCAGT ATGCCTCCTT TTTGTGTTTT CAGCACTTCT GGAGTATGCA GCTGTAAATT
1081TTGTATCAAG ACAACACAAA GAACTTCTGA GATTTCGACG AAAGAGAAAG AATAAGACAG
1141AAGCCTTTGC ACTGGAGAAG TTTTACCGTT TCTCAGATAT GGATGATGAG GTAAGGGAAA
1201GCCGATTCAG CTTCACAGCC TATGGAATGG GACCATGTCT ACAAGCAAAG GATGGCATGA
1261CTCCAAAGGG CCCCAACCAC CCTGTCCAGG TAATGCCAAA AAGTCCTGAT GAAATGAGGA
1321AGGTCTTTAT CGACCGGGCC AAGAAGATTG ATACCATCTC CCGAGCCTGC TTCCCATTAG
1381CTTTTTTGAT TTTTAATATT TTCTACTGGG TTATCTATAA AATTCTTAGG CATGAGGATA
1441TTCATCAGCA GCAAGATTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
1501gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)