Construct: ORF ccsbBroadEn_07321

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002167.1_s300c1, BRDN0000389079
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SQSTM1 (8878)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8878 SQSTM1 sequestosome 1 NM_001142298.2 99.8% 100% 624C>T;684G>A
2 human 8878 SQSTM1 sequestosome 1 NM_001142299.2 99.8% 100% 624C>T;684G>A
3 human 8878 SQSTM1 sequestosome 1 NM_003900.5 80.7% 80.9% 1_252del;876C>T;936G>A
4 mouse 18412 Sqstm1 sequestosome 1 NM_011018.3 69.5% 73.3% (many diffs)
5 mouse 18412 Sqstm1 sequestosome 1 NM_001290769.1 61.5% 64.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1134
ORF length:
1068
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CATGTCCTAC GTGAAGGATG ACATCTTCCG AATCTACATT AAAGAGAAAA
121AAGAGTGCCG GCGGGACCAC CGCCCACCGT GTGCTCAGGA GGCGCCCCGC AACATGGTGC
181ACCCCAATGT GATCTGCGAT GGCTGCAATG GGCCTGTGGT AGGAACCCGC TACAAGTGCA
241GCGTCTGCCC AGACTACGAC TTGTGTAGCG TCTGCGAGGG AAAGGGCTTG CACCGGGGGC
301ACACCAAGCT CGCATTCCCC AGCCCCTTCG GGCACCTGTC TGAGGGCTTC TCGCACAGCC
361GCTGGCTCCG GAAGGTGAAA CACGGACACT TCGGGTGGCC AGGATGGGAA ATGGGTCCAC
421CAGGAAACTG GAGCCCACGT CCTCCTCGTG CAGGGGAGGC CCGCCCTGGC CCCACGGCAG
481AATCAGCTTC TGGTCCATCG GAGGATCCGA GTGTGAATTT CCTGAAGAAC GTTGGGGAGA
541GTGTGGCAGC TGCCCTTAGC CCTCTGGGCA TTGAAGTTGA TATCGATGTG GAGCACGGAG
601GGAAAAGAAG CCGCCTGACC CCCGTCTCTC CAGAGAGTTC CAGCACAGAG GAGAAGAGCA
661GCTCACAGCC AAGCAGCTGC TGCTCTGATC CCAGCAAGCC GGGTGGGAAT GTTGAGGGCG
721CCACGCAGTC TCTGGCGGAG CAGATGAGAA AGATCGCCTT GGAGTCCGAG GGGCGCCCTG
781AGGAACAGAT GGAGTCGGAT AACTGTTCAG GAGGAGATGA TGACTGGACC CATCTGTCTT
841CAAAAGAAGT GGACCCGTCT ACAGGTGAAC TCCAGTCCCT ACAGATGCCA GAATCCGAAG
901GGCCAAGCTC TCTGGACCCC TCCCAGGAGG GACCCACAGG GCTGAAGGAA GCTGCCTTGT
961ACCCACATCT CCCGCCAGAG GCTGACCCGC GGCTGATTGA GTCCCTCTCC CAGATGCTGT
1021CCATGGGCTT CTCTGATGAA GGCGGCTGGC TCACCAGGCT CCTGCAGACC AAGAACTATG
1081ACATCGGAGC GGCTCTGGAC ACCATCCAGT ATTCAAAGCA TCCCCCGCCG TTGTGCCCAA
1141CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)