Construct: ORF ccsbBroadEn_07395

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012327.1_s300c1, BRDN0000385488
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFTUD2 (9343)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9343 EFTUD2 elongation factor Tu GTP bi... NM_001258353.1 99.9% 99.8% 962G>T
2 human 9343 EFTUD2 elongation factor Tu GTP bi... NM_004247.4 99.9% 99.8% 962G>T
3 human 9343 EFTUD2 elongation factor Tu GTP bi... NM_001258354.1 98.9% 98.8% 424_425ins30;932G>T
4 human 9343 EFTUD2 elongation factor Tu GTP bi... NM_001142605.1 96.3% 96.2% 0_1ins105;857G>T
5 human 9343 EFTUD2 elongation factor Tu GTP bi... XR_934602.3 81.9% (many diffs)
6 mouse 20624 Eftud2 elongation factor Tu GTP bi... NM_001109995.1 91.5% 99.2% (many diffs)
7 mouse 20624 Eftud2 elongation factor Tu GTP bi... NM_011431.3 91.4% 99.1% (many diffs)
8 mouse 20624 Eftud2 elongation factor Tu GTP bi... XM_006532665.3 91.2% 99% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2982
ORF length:
2916
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA TACCGACTTA TATGATGAGT TTGGGAATTA TATTGGACCA GAGCTTGATT
121CTGATGAAGA TGATGATGAA TTGGGTAGAG AGACCAAAGA TCTTGATGAG ATGGATGATG
181ATGACGACGA CGATGACGTA GGAGATCATG ACGATGACCA CCCTGGGATG GAGGTGGTGC
241TGCATGAGGA CAAGAAGTAC TACCCAACAG CCGAGGAGGT GTATGGTCCT GAGGTGGAGA
301CCATAGTTCA AGAGGAAGAC ACTCAGCCTC TCACAGAACC CATTATTAAG CCAGTGAAAA
361CCAAGAAATT CACTCTGATG GAGCAGACAT TACCTGTTAC GGTGTATGAG ATGGATTTCT
421TGGCGGATCT GATGGATAAC TCAGAGCTCA TCAGAAATGT GACCCTTTGT GGACATCTCC
481ACCATGGCAA GACATGTTTT GTGGATTGTT TAATTGAACA GACTCACCCG GAAATCAGAA
541AGCGCTATGA CCAAGATCTG TGCTATACTG ACATCCTCTT CACAGAGCAA GAGAGAGGTG
601TAGGCATCAA AAGCACTCCT GTGACAGTGG TCTTGCCAGA CACCAAAGGA AAATCTTATC
661TCTTCAATAT CATGGACACT CCAGGACATG TGAATTTCTC TGATGAGGTC ACAGCTGGCT
721TGCGCATCTC AGATGGAGTG GTCCTTTTCA TTGATGCTGC TGAGGGGGTG ATGCTGAACA
781CAGAGCGGCT GATCAAGCAT GCGGTGCAGG AGAGGCTGGC AGTCACTGTG TGCATCAACA
841AGATTGACCG GCTGATCCTG GAGCTGAAGC TGCCTCCAAC TGATGCTTAT TACAAGCTGC
901GCCACATTGT GGATGAGGTC AATGGATTAA TAAGCATGTA TTCCACTGAT GAGAACCTGA
961TCCTTTCCCC ACTCCTGGGT AACGTCTGCT TCTCCAGCTC CCAGTACAGC ATCTGCTTCA
1021CGCTGGTCTC CTTTGCCAAG ATCTATGCCG ACACCTTTGG TGACATTAAT TACCAAGAAT
1081TTGCTAAAAG ACTCTGGGGT GACATCTACT TCAACCCTAA GACGCGAAAG TTCACCAAAA
1141AGGCCCCAAC TAGCAGCTCC CAGAGAAGTT TCGTGGAGTT TATCTTGGAG CCTCTTTATA
1201AGATCCTCGC CCAGGTTGTA GGTGACGTGG ACACCAGCCT CCCACGGACC CTAGACGAGC
1261TTGGCATCCA CCTGACGAAG GAGGAGCTGA AGCTGAACAT CCGCCCCTTG CTCAGGCTGG
1321TCTGCAAAAA GTTCTTTGGC GAGTTCACAG GCTTTGTGGA CATGTGTGTG CAGCATATCC
1381CTTCTCCAAA GGTGGGCGCC AAGCCCAAGA TTGAGCACAC CTACACCGGT GGTGTGGACT
1441CCGACCTCGG CGAGGCTATG AGTGACTGTG ACCCTGATGG CCCCCTGATG TGCCACACTA
1501CTAAGATGTA CAGCACAGAT GATGGAGTCC AGTTTCACGC CTTTGGCCGG GTGCTGAGTG
1561GCACCATTCA TGCTGGGCAG CCTGTGAAGG TACTGGGGGA GAACTACACC CTGGAGGATG
1621AGGAAGACTC CCAGATATGC ACCGTGGGCC GCCTTTGGAT CTCTGTGGCC AGGTACCACA
1681TCGAGGTGAA CCGTGTTCCT GCTGGCAACT GGGTTCTGAT TGAAGGTGTT GATCAACCAA
1741TTGTGAAGAC AGCAACCATA ACCGAACCCC GAGGCAATGA GGAGGCTCAG ATTTTCCGAC
1801CCTTGAAGTT CAATACCACA TCTGTTATCA AGATTGCTGT GGAGCCAGTC AACCCCTCAG
1861AGCTGCCCAA GATGCTTGAT GGCCTGCGCA AGGTCAACAA GAGCTATCCA TCCCTCACCA
1921CCAAGGTGGA GGAGTCTGGC GAGCATGTGA TCCTGGGCAC TGGGGAGCTC TACCTGGACT
1981GTGTGATGCA TGATTTGCGG AAGATGTACT CAGAGATAGA CATCAAGGTG GCTGACCCAG
2041TTGTCACGTT TTGTGAGACG GTGGTGGAAA CATCCTCCCT CAAGTGCTTT GCTGAAACGC
2101CTAATAAGAA GAACAAGATC ACCATGATTG CTGAGCCTCT TGAGAAGGGC CTGGCAGAGG
2161ACATAGAGAA TGAGGTGGTC CAGATTACGT GGAACAGGAA GAAGCTGGGA GAGTTCTTCC
2221AGACCAAGTA CGATTGGGAT CTGCTGGCTG CCCGTTCCAT CTGGGCTTTT GGCCCTGATG
2281CGACTGGCCC CAACATTCTG GTGGATGATA CTCTGCCCTC TGAGGTGGAC AAGGCTCTTC
2341TTGGTTCAGT GAAGGACAGC ATCGTTCAAG GTTTCCAGTG GGGAACCAGG GAGGGCCCCC
2401TCTGTGATGA ATTGATTCGG AATGTCAAGT TTAAGATCCT GGATGCGGTG GTTGCCCAGG
2461AGCCCCTGCA CCGGGGCGGG GGCCAGATCA TCCCCACAGC CAGGAGAGTC GTCTACTCTG
2521CCTTCCTCAT GGCTACTCCT CGTCTGATGG AGCCTTACTA CTTTGTAGAG GTCCAGGCCC
2581CTGCAGATTG CGTCTCTGCA GTTTATACCG TCCTGGCCAG GCGCAGGGGG CACGTGACTC
2641AGGATGCACC CATCCCAGGC TCCCCTCTGT ACACCATCAA AGCTTTTATC CCGGCCATCG
2701ACTCTTTTGG CTTTGAGACT GATCTCCGGA CTCACACCCA GGGACAAGCC TTTTCTCTGT
2761CTGTCTTCCA CCACTGGCAG ATTGTGCCTG GTGATCCCCT GGACAAGAGC ATTGTCATCC
2821GCCCCTTGGA GCCACAGCCA GCTCCTCACC TGGCCCGGGA ATTCATGATC AAAACCCGCC
2881GTAGGAAGGG CCTCAGTGAA GATGTGAGCA TCAGCAAATT CTTCGATGAT CCTATGTTGC
2941TGGAACTTGC CAAACAGGAT GTTGTGCTCA ATTACCCCAT GTGCCCAACT TTCTTGTACA
3001AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)

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