Construct: ORF ccsbBroadEn_07421

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015275.1_s300c1, BRDN0000456229
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCYT1B (9468)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9468 PCYT1B phosphate cytidylyltransfer... NM_001163264.2 99.9% 99.7% 10C>A
2 human 9468 PCYT1B phosphate cytidylyltransfer... NM_004845.5 93.1% 90.8% (many diffs)
3 human 9468 PCYT1B phosphate cytidylyltransfer... NM_001163265.2 80% 78.3% (many diffs)
4 human 9468 PCYT1B phosphate cytidylyltransfer... XM_017029977.1 77.7% 77.7% 0_1ins234
5 mouse 236899 Pcyt1b phosphate cytidylyltransfer... NM_177546.2 86.8% 93.4% (many diffs)
6 mouse 236899 Pcyt1b phosphate cytidylyltransfer... XM_006527985.3 86.7% 92.3% (many diffs)
7 mouse 236899 Pcyt1b phosphate cytidylyltransfer... NM_211138.1 84.5% 89.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1122
ORF length:
1053
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTAGGCAAT CAGGAGTGCA TCATGGAAGA GGACAATCGC GCTCCCCAAT
121TGTGGCGCAA GACCCTGACT GCACCTGCCC CATTTGCTGA TGAAACCAAC TGCCAGTGTC
181AAGCACCCCA TGAAAAACTG ACCATTGCTC AGGCCCGCTT AGGAACACCA GCTGACAGGC
241CTGTCAGAGT ATACGCCGAT GGAATATTTG ACCTCTTCCA CTCAGGTCAT GCAAGAGCCC
301TTATGCAAGC AAAAACACTG TTTCCCAACA GCTACTTGTT GGTAGGAGTT TGCAGTGATG
361ATCTCACCCA CAAATTCAAA GGTTTCACCG TGATGAATGA AGCCGAGAGA TACGAAGCTC
421TCAGACACTG TCGCTACGTA GACGAAGTTA TCAGAGATGC TCCCTGGACA CTCACGCCAG
481AGTTTCTGGA AAAACACAAG ATTGACTTTG TGGCTCATGA TGACATTCCG TATTCCTCTG
541CTGGCTCTGA TGATGTTTAC AAGCACATAA AGGAAGCAGG GATGTTCGTT CCAACGCAGA
601GAACAGAAGG CATCTCAACA TCGGACATCA TTACCAGAAT TGTTCGTGAC TATGATGTTT
661ATGCCCGACG TAACCTCCAG AGAGGGTATA CAGCCAAGGA ACTGAATGTC AGCTTTATAA
721ATGAGAAGAG GTACCGTTTC CAGAACCAAG TGGACAAAAT GAAGGAAAAA GTCAAGAATG
781TGGAGGAAAG ATCAAAGGAA TTTGTGAACA GAGTGGAAGA AAAGAGCCAT GATCTAATTC
841AAAAGTGGGA AGAGAAGTCA AGGGAATTCA TTGGCAACTT CCTAGAACTG TTTGGACCTG
901ATGGAGCATG GAAGCAGATG TTCCAGGAGA GGAGCAGCCG GATGCTGCAG GCCTTATCCC
961CGAAGCAGAG CCCTGTGAGC AGCCCAACCC GGAGCCGGTC CCCTTCCCGC TCCCCATCGC
1021CCACCTTCTC ATGGCTTCCA CTCAAAACCT CACCCCCTTC CTCACCCAAA GCAGCCTCAG
1081CCTCTATCAG CAGCATGAGC GAGGGGGATG AGGATGAAAA GTTGCCAACT TTCTTGTACA
1141AAGTTGGcat tataagaaag cattgcttat caatttgttg caacgaac

Download FASTA (ORF) (Full)