Transcript: Mouse NM_211138.1

Mus musculus phosphate cytidylyltransferase 1, choline, beta isoform (Pcyt1b), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Pcyt1b (236899)
Length:
4875
CDS:
249..1358

Additional Resources:

NCBI RefSeq record:
NM_211138.1
NBCI Gene record:
Pcyt1b (236899)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_211138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000111345 CCAGGTACAAACAGACACTTA pLKO.1 3565 3UTR 100% 4.950 3.960 N Pcyt1b n/a
2 TRCN0000111349 CGCTTAGGAACACCAGTTGAT pLKO.1 450 CDS 100% 4.950 3.465 N Pcyt1b n/a
3 TRCN0000111347 GCTACTTGTTGGTAGGAGTTT pLKO.1 565 CDS 100% 4.950 3.465 N Pcyt1b n/a
4 TRCN0000111348 CCTGTTAGAGTGTATGCAGAT pLKO.1 474 CDS 100% 4.050 2.835 N Pcyt1b n/a
5 TRCN0000111346 GCATGTTTGTTCCAACACAAA pLKO.1 814 CDS 100% 0.495 0.347 N Pcyt1b n/a
6 TRCN0000035679 CCCAACAGCTACTTGTTGGTA pLKO.1 558 CDS 100% 0.300 0.210 N PCYT1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_211138.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07421 pDONR223 100% 84.5% 89.1% None (many diffs) n/a
2 ccsbBroad304_07421 pLX_304 0% 84.5% 89.1% V5 (many diffs) n/a
3 TRCN0000478025 GTACACCGCTTATTTCTGACAATA pLX_317 21.7% 84.5% 89.1% V5 (many diffs) n/a
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