Construct: ORF ccsbBroadEn_07474

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001221.1_s300c1, BRDN0000395215
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ECE2 (9718)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9718 ECE2 endothelin converting enzyme 2 NM_001037324.3 99.9% 99.8% 630C>T;959T>C
2 human 9718 ECE2 endothelin converting enzyme 2 NM_001100121.2 96.1% 96% 40_126del;717C>T;1046T>C
3 human 9718 ECE2 endothelin converting enzyme 2 NM_001100120.2 90.6% 90.6% 40_264del;855C>T;1184T>C
4 human 110599583 EEF1AKMT4-ECE2 EEF1AKMT4-ECE2 readthrough NM_014693.4 82.7% 81.8% (many diffs)
5 mouse 107522 Ece2 endothelin converting enzyme 2 NM_177942.1 88.7% 92.7% (many diffs)
6 mouse 107522 Ece2 endothelin converting enzyme 2 NM_139293.2 85.6% 89.2% (many diffs)
7 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521693.3 85.1% 79.7% (many diffs)
8 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521691.3 82.2% 76.8% (many diffs)
9 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521696.3 80.3% 82.3% (many diffs)
10 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521692.3 80.2% 80.1% (many diffs)
11 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521697.3 78.5% 81.8% (many diffs)
12 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521694.3 75.9% 78.2% (many diffs)
13 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521695.3 75.8% 78% (many diffs)
14 mouse 107522 Ece2 endothelin converting enzyme 2 NM_177940.1 73.4% 76.4% (many diffs)
15 mouse 107522 Ece2 endothelin converting enzyme 2 NM_177941.1 71.6% 74% (many diffs)
16 mouse 107522 Ece2 endothelin converting enzyme 2 XM_017316848.1 64% 59% (many diffs)
17 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521698.3 60.3% 62.2% (many diffs)
18 mouse 107522 Ece2 endothelin converting enzyme 2 XM_006521700.3 56.4% 51.1% (many diffs)
19 mouse 107522 Ece2 endothelin converting enzyme 2 XM_011245802.2 56.4% 51.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2277
ORF length:
2208
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACTAT GAACGTCGCG CTGCAGGAGC TGGGAGCTGG CAGCAACGTG GGATTCCAGA
121AGGGGACAAG ACAGCTGTTA GGCTCACGCA CGCAGCTGGA GCTGGTCTTA GCAGGTGCCT
181CTCTACTGCT GGCTGCACTG CTTCTGGGCT GCCTTGTGGC CCTAGGGGTC CAGTACCACA
241GAGACCCATC CCACAGCACC TGCCTTACAG AGGCCTGCAT TCGAGTGGCT GGAAAAATCC
301TGGAGTCCCT GGACCGAGGG GTGAGCCCCT GTGAGGACTT TTACCAGTTC TCCTGTGGGG
361GCTGGATTCG GAGGAACCCC CTGCCCGATG GGCGTTCTCG CTGGAACACC TTCAACAGCC
421TCTGGGACCA AAACCAGGCC ATACTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCCA
481GCAGTGAAGC TGAGCAGAAG ACACAGCGCT TCTACCTATC TTGCCTACAG GTGGAGCGCA
541TTGAGGAGCT GGGAGCCCAG CCACTGAGAG ACCTCATTGA GAAGATTGGT GGTTGGAACA
601TTACGGGGCC CTGGGACCAG GACAACTTTA TGGAGGTGTT GAAGGCAGTA GCAGGGACCT
661ACAGGGCCAC CCCATTCTTC ACCGTCTACA TCAGTGCTGA CTCTAAGAGT TCCAACAGCA
721ATGTTATCCA GGTGGACCAG TCTGGGCTCT TTCTGCCCTC TCGGGATTAC TACTTAAACA
781GAACTGCCAA TGAGAAAGTG CTCACTGCCT ATCTGGATTA CATGGAGGAA CTGGGGATGC
841TGCTGGGTGG GCGGCCCACC TCCACGAGGG AGCAGATGCA GCAGGTGCTG GAGTTGGAGA
901TACAGCTGGC CAACATCACA GTGCCCCAGG ACCAGCGGCG CGACGAGGAG AAGATCTACC
961ACAAGATGAG CATTTCGGAG CTGCAGGCTC TGGCGCCCTC CATGGACTGG CTTGAGTTCC
1021TGTCTTCCTT GCTGTCACCA TTGGAGTTGA GTGACTCTGA GCCTGTGGTG GTGTATGGGA
1081TGGATTATTT GCAGCAGGTG TCAGAGCTCA TCAACCGCAC GGAACCAAGC ATCCTGAACA
1141ATTACCTGAT CTGGAACCTG GTGCAAAAGA CAACCTCAAG CCTGGACCGA CGCTTTGAGT
1201CTGCACAAGA GAAGCTGCTG GAGACCCTCT ATGGCACTAA GAAGTCCTGT GTGCCGAGGT
1261GGCAGACCTG CATCTCCAAC ACGGATGACG CCCTTGGCTT TGCTTTGGGG TCCCTCTTCG
1321TGAAGGCCAC GTTTGACCGG CAAAGCAAAG AAATTGCAGA GGGGATGATC AGCGAAATCC
1381GGACCGCATT TGAGGAGGCC CTGGGACAGC TGGTTTGGAT GGATGAGAAG ACCCGCCAGG
1441CAGCCAAGGA GAAAGCAGAT GCCATCTATG ATATGATTGG TTTCCCAGAC TTTATCCTGG
1501AGCCCAAAGA GCTGGATGAT GTTTATGACG GGTACGAAAT TTCTGAAGAT TCTTTCTTCC
1561AAAACATGTT GAATTTGTAC AACTTCTCTG CCAAGGTTAT GGCTGACCAG CTCCGCAAGC
1621CTCCCAGCCG AGACCAGTGG AGCATGACCC CCCAGACAGT GAATGCCTAC TACCTTCCAA
1681CTAAGAATGA GATCGTCTTC CCCGCTGGCA TCCTGCAGGC CCCCTTCTAT GCCCGCAACC
1741ACCCCAAGGC CCTGAACTTC GGTGGCATCG GTGTGGTCAT GGGCCATGAG TTGACGCATG
1801CCTTTGATGA CCAAGGGCGC GAGTATGACA AAGAAGGGAA CCTGCGGCCC TGGTGGCAGA
1861ATGAGTCCCT GGCAGCCTTC CGGAACCACA CGGCCTGCAT GGAGGAACAG TACAATCAAT
1921ACCAGGTCAA TGGGGAGAGG CTCAACGGCC GCCAGACGCT GGGGGAGAAC ATTGCTGACA
1981ACGGGGGGCT GAAGGCTGCC TACAATGCTT ACAAAGCATG GCTGAGAAAG CATGGGGAGG
2041AGCAGCAACT GCCAGCCGTG GGGCTCACCA ACCACCAGCT CTTCTTCGTG GGATTTGCCC
2101AGGTGTGGTG CTCGGTCCGC ACACCAGAGA GCTCTCACGA GGGGCTGGTG ACCGACCCCC
2161ACAGCCCTGC CCGCTTCCGC GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC TTCCTGCGGC
2221ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACCCAGGGCA GCTGTGTGAG GTGTGGTTGC
2281CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
2341aac

Download FASTA (ORF) (Full)