Construct: ORF ccsbBroadEn_07499

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007112.1_s300c1, BRDN0000395487
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MED24 (9862)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9862 MED24 mediator complex subunit 24 NM_014815.4 99.9% 100% 1161G>A;2886C>T
2 human 9862 MED24 mediator complex subunit 24 NM_001079518.2 98.6% 98.6% 211_212ins39;1122G>A;2847C>T
3 human 9862 MED24 mediator complex subunit 24 NM_001267797.2 98.6% 98.6% 211_212ins39;1122G>A;2847C>T
4 human 9862 MED24 mediator complex subunit 24 NM_001330211.2 98% 98.1% 560_616del;1218G>A;2943C>T
5 human 9862 MED24 mediator complex subunit 24 NR_052017.2 83% (many diffs)
6 mouse 23989 Med24 mediator complex subunit 24 NM_011869.2 88.7% 94.2% (many diffs)
7 mouse 23989 Med24 mediator complex subunit 24 XM_006533305.2 88.7% 94.2% (many diffs)
8 mouse 23989 Med24 mediator complex subunit 24 XM_006533302.1 87% 92.4% (many diffs)
9 mouse 23989 Med24 mediator complex subunit 24 XM_006533303.2 87% 92.4% (many diffs)
10 mouse 23989 Med24 mediator complex subunit 24 XM_006533304.2 87% 92.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3033
ORF length:
2967
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GGTGGTCAAC CTGAAGCAAG CCATTTTGCA AGCCTGGAAG GAGCGCTGGA
121GTGACTACCA ATGGGCAATC AACATGAAGA AATTCTTTCC TAAAGGAGCC ACCTGGGATA
181TTCTCAACCT GGCAGATGCG TTACTAGAGC AGGCCATGAT TGGACCATCC CCCAATCCTC
241TCATCTTGTC CTACCTGAAG TATGCCATTA GTTCCCAGAT GGTGTCCTAC TCTTCTGTCC
301TCACAGCCAT CAGTAAGTTT GATGACTTTT CTCGGGACCT GTGTGTCCAG GCATTGCTGG
361ACATCATGGA CATGTTTTGT GACCGTCTGA GCTGTCACGG CAAAGCAGAG GAATGCATCG
421GACTGTGCCG AGCCCTTCTT AGCGCCCTCC ACTGGCTGCT GCGCTGCACG GCAGCCTCTG
481CAGAGCGGCT GCGGGAGGGG CTGGAGGCCG GCACTCCAGC CGCTGGGGAG AAGCAGCTTG
541CCATGTGCCT TCAGCGCCTG GAGAAAACCC TCAGCAGCAC CAAGAACCGG GCCCTGCTGC
601ACATCGCCAA ACTAGAGGAG GCCTCTTCTT GGACTGCCAT CGAGCATTCT CTCTTGAAAC
661TTGGAGAGAT CCTGGCCAAT CTCAGCAACC CGCAGCTCCG GAGTCAGGCC GAGCAGTGTG
721GCACCCTCAT TAGGAGCATC CCCACGATGC TGTCTGTGCA TGCGGAGCAG ATGCACAAGA
781CCGGCTTCCC CACTGTCCAC GCCGTGATCC TGCTCGAGGG CACCATGAAC CTGACAGGCG
841AGACGCAGTC CCTGGTGGAG CAGCTGACGA TGGTGAAGCG CATGCAGCAT ATCCCCACCC
901CACTTTTTGT CCTGGAGATC TGGAAAGCTT GCTTCGTGGG GCTCATTGAG TCTCCCGAGG
961GTACGGAGGA GCTCAAGTGG ACAGCTTTCA CTTTCCTCAA GATTCCACAG GTTTTGGTGA
1021AGTTGAAGAA GTACTCTCAT GGAGACAAGG ACTTCACTGA GGATGTCAAC TGTGCTTTTG
1081AGTTCCTGCT GAAGCTCACC CCCTTGTTGG ACAAAGCTGA CCAGCGCTGC AACTGTGACT
1141GTACAAACTT CCTGCTCCAA GAATGTGGCA AGCAGGGGCT TCTGTCTGAG GCCAGCGTCA
1201ACAACCTTAT GGCTAAGCGC AAAGCAGACC GAGAGCACGC ACCCCAGCAG AAATCGGGAG
1261AGAATGCCAA CATCCAGCCC AACATCCAGC TGATCCTCCG GGCGGAGCCC ACTGTCACAA
1321ACATCCTCAA GACGATGGAT GCAGACCACT CTAAGTCACC GGAGGGACTG CTGGGAGTCC
1381TGGGCCACAT GCTGTCCGGG AAGAGTCTGG ACTTGCTGCT GGCTGCCGCC GCCGCCACTG
1441GAAAGCTGAA ATCCTTCGCC CGGAAATTCA TCAATTTGAA TGAATTCACA ACCTATGGCA
1501GCGAAGAAAG CACCAAACCG GCCTCCGTCC GGGCCCTGCT GTTTGACATC TCCTTCCTCA
1561TGCTGTGCCA TGTGGCCCAG ACCTATGGTT CAGAGGTGAT TCTGTCCGAG TCGCGCACAG
1621GAGCTGAGGT GCCCTTCTTC GAGACCTGGA TGCAGACCTG CATGCCTGAG GAGGGCAAGA
1681TCCTGAACCC TGACCACCCC TGCTTCCGCC CCGACTCCAC CAAAGTGGAG TCCCTGGTGG
1741CCCTGCTCAA CAACTCCTCG GAGATGAAGC TAGTGCAGAT GAAGTGGCAT GAGGCCTGTC
1801TCAGCATCTC AGCCGCCATC TTGGAAATCC TCAATGCCTG GGAGAATGGG GTCCTGGCCT
1861TCGAGTCCAT CCAGAAAATC ACTGATAACA TCAAAGGGAA GGTATGCAGT CTGGCGGTGT
1921GTGCTGTGGC TTGGCTTGTG GCCCACGTCC GGATGCTGGG GCTGGATGAG CGTGAGAAGT
1981CGCTGCAGAT GATCCGCCAG CTGGCAGGGC CACTGTTTAG TGAGAACACC CTGCAGTTCT
2041ACAATGAGAG GGTGGTGATC ATGAACTCGA TCCTGGAGCG CATGTGTGCC GACGTGCTGC
2101AGCAGACAGC CACGCAGATC AAGTTTCCCT CCACCGGGGT GGACACAATG CCCTACTGGA
2161ACCTGCTGCC CCCCAAGCGG CCCATCAAAG AGGTGCTGAC GGACATTTTT GCCAAGGTGC
2221TGGAGAAGGG CTGGGTGGAC AGCCGCTCCA TCCACATCTT TGACACCCTG CTGCACATGG
2281GCGGCGTCTA CTGGTTCTGC AACAACCTGA TTAAGGAGCT GCTGAAGGAG ACGCGGAAGG
2341AGCACACGCT GCGGGCAGTG GAGCTGCTCT ACTCCATCTT CTGCCTGGAC ATGCAGCAAG
2401TGACCCTGGT CCTGCTGGGC CACATCCTAC CTGGCCTGCT CACTGACTCC TCCAAGTGGC
2461ACAGCCTCAT GGACCCCCCG GGCACTGCTC TTGCCAAGCT GGCCGTGTGG TGTGCCCTCA
2521GTTCCTACTC CTCCCACAAG GGACAGGCGT CCACCCGCCA GAAGAAGAGA CACCGCGAAG
2581ACATTGAGGA TTATATCAGC CTCTTCCCCC TGGACGATGT GCAGCCTTCG AAGTTGATGC
2641GACTGCTGAG CTCTAATGAG GACGATGCCA ACATCCTTTC GAGCCCCACA GACCGATCCA
2701TGAGCAGCTC CCTCTCAGCC TCTCAGCTCC ACACGGTCAA CATGCGGGAC CCTCTGAACC
2761GAGTCCTGGC CAACCTGTTC CTGCTCATCT CCTCCATCCT GGGGTCTCGC ACCGCTGGCC
2821CCCACACCCA GTTCGTGCAG TGGTTCATGG AGGAGTGTGT GGACTGCCTG GAGCAGGGTG
2881GCCGTGGCAG CGTCCTGCAG TTCATGCCCT TCACCACCGT GTCGGAACTG GTGAAGGTGT
2941CAGCCATGTC TAGCCCCAAG GTGGTTCTGG CCATCACGGA CCTCAGCCTG CCCCTGGGCC
3001GCCAGGTGGC TGCTAAAGCC ATTGCTGCAC TCTGCCCAAC TTTCTTGTAC AAAGTtggca
3061ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)